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@#Objective In this study,the clinical data of single-center acute posterior circulation stroke patients were analyzed to compare the efficacy of TOAST classification for large artery atherosclerosis (LAA) and cardioembolism (CE) stroke patients after intravascular treatment difference.Methods Patient data comes from the advanced stroke center of the First Affiliated Hospital of Shihezi University School of Medicine,Xinjiang,China.According to the TOAST classification,the 98 people included were divided into LAA group and CE group.The mTICI score was used to evaluate vascular recanalization,the 90 day mRS score was used to evaluate the prognosis,the incidence of intracranial hemorrhage conversion within 72 hours and 90 day mortality Assess the safety of intravascular treatment.Results Among the 98 patients included in the study,79 were LAA stroke patients and 19 were CE patients.Compared with CE patients,LAA stroke patients are younger (60.08±12.64 vs 71.05±10.42,P=0.001),have a higher prevalence of hyperlipidemia (55.7% vs 22.1%,P=0.014),and preoperative systolic blood pressure higher (149.25±21.24 vs 134.79±22.83,P=0.010),lower NIHSS score (17.3 vs 22.2,P=0.025),better collateral circulation (ASITN/SIR score of 2~4 ratio,63.3% vs 21.1 %,P=0.002),the angioplasty rate is higher (31.6% vs 5.3%,P=0.040).However,the EVT recanalization rate (87.5% vs 84.2%,P=1.000) of the two groups of patients,the 90 day neurological function recovery rate (mRS score 0~2) (58.2% vs 36.8%,P=0.093),The incidence of postoperative intracranial hemorrhage transformation (1.3% vs 0%,P=1.000) and the 90 day stroke-related mortality (20.3% vs 42.1%,P=0.091) were not statistically significant.Conclusion s Although there were differences in baseline clinical data and clinical characteristics of EVT treatment between LAA and CE in posterior circulation,there was no significant difference in prognosis.
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Objective Recent studies have indicated that early brain injury is the leading cause of death in patients with subarachnoid hemorrhage ( SAH) .Our study investigated the role of aminoguanidine ( AG) in early brain injury after SAH . Methods Sixty-eight male SD rats were equally randomized into four groups of equal number :control, sham, SAH, and AG.The animals in the sham group were injected with isotonic saline solution , while those of the latter two groups with femoral artery blood ( FAB) and FAB+AG, respectively, into the pre-chiasmatic cistern to induce SAH. At 24 hours after modeling , all the rats were killed for HE staining , obtainment of behavioral neurological assessment ( BNA ) scores by Garcia, measurement of the apoptosis of neurons by TUNEL , and de-termination of the expressions of the iNOS and NSE proteins by West-ern blot. Results The results of HE staining showed the presence of more red blood cells in the subarachnoid cavity of the rats in the SAH group, with a significantly decreased BNA score ( 14.47 ± 0.62) as compared with those in the control (17.94 ±0.24), sham (17.59 ±0.51), and AG group (15.71 ±0.47) (P<0.05). The rate of positive cells was remarkably higher in the SAH group ([42.38 ±2.38]%) than in the control ([6.35 ±0.94]%), sham ([6.85 ±0.69]%), and AG group ([30.48 ±2.89]%) ( P<0.01), with significant differences among the latter three groups (P<0.05).The expressions of iNOS and NSE were markedly higher in the SAH group ([3.86 ±0.07] and [1.59 ±0.06]) than in the control (0 and[0.35 ±0.09]), sham ([2.96 ±0.34] and [0.38 ±0.08]), and AG group ([3.41 ±0.04] and [0.70 ±0.12]) ( P<0.05).Both the expression levels of iNOS and NSE were positively correlated with the rate of positive cells (r=0 .879 and 0.935, P<0.01). Conclusion AG can alleviate early brain injury after SAH in SD rats by improving the neuro-ethologic function , suppressing the apoptosis of neurons , and reducing the expressions of iNOS and NSE .
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Objective To detect time-dependent change of neuritin expression in brain tissues after traumatic brain injury and discuss the effect of neuritin after brain damage occurred. Methods Forty-two rats were divided into normal group, control and experimental group. According to the postoperative time divided into 6 subgroups, including 6 hours group, 12 hours group, 24 hours group, 3 days group, 7 days group and 14 days group. Immunohistochemical and western-blot were used to detected the protein expression levels of neuritin. Results The immunohistochemical staining indicated that the positive expression of neuritin was strong in the cytomembrane and cytoplasms of the neurons, with a higher intensity, 6 hours after the operation. 12 hours after the operation last to the seventh day, the neurons with the strongest positive expression, is significantly higher than control group and normal group, significant decrease on the fourteenth day. The result of western-blot indicated that the level of neuritin protein sharply increased at 6 hours, reached the peak on 24 hours and after lasted to the seventh day, significantly higher than control group and normal group (P < 0.01), significant decrease on the fourteenth day (P < 0.05). Conclusion Up-regulation of neuritin in cerebral contusion tissues may play an important role after traumatic brain injury.