ABSTRACT
In recent years， neurotoxicity caused by traditional Chinese medicine （TCM） has frequently occurred and has become one of the important factors restricting the development and application of TCM. TCM contains active components and its dosage-effect relationship is the key to determine its pharmacological activity and toxic effects. Among them， the endogenous toxic components include alkaloids， glycosides， diterpenoids， animal and plant toxic proteins and heavy metals， and so on； exogenous toxic components mainly refer to some harmful elements and pesticide residues during the cultivation， processing， transportation and storage of medicinal materials that are not synthesized by themselves. Effect on the processes such as oxidative stress， inflammation， ion exchange， and energy metabolism may be important mechanisms of TCM-induced neurotoxicity. Neural cells， myelin cells， axons and neurotransmitter systems are common targets of TCM-induced neurotoxicity. In the future， we can use modern research methods and big data mining means to establish a safety evaluation mode of “toxic symptoms-poisoning dose-toxic original agent-detoxification scheme” with the basic component group of toxic substances as the core， so as to provide support for development and clinical intervention of neurotoxic traditional Chinese medicine.
ABSTRACT
We studid the research status and network analysis of health food containing Angelica sinensis in the past 20 years with the methods of data mining. The key information of health food containing Angelica sinensis was obtained in State Food and Drug Administration (SFDA) webpage, using Bibexcel analysis software generated co-occurrence matrix, and using Ucinet 6.0 Net Draw software drawed the social network graph according to the centrality. The data was calculated by SPSS19.0 software analysis. The results showed that 539 health food containing Angelica sinensis were successfully registered in 1997-2016 years, and the annual number of registration was the overall growth trend, there is a certain gap in R & D ability and level of health food containing Angelica sinensis with Angelica health food, brand advantage had not been established in health food containing Angelica sinensis; Health food containing Angelica sinensis form tend to preparation was very obvious such as capsule and oral liquid. The function of health food containing Angelica sinensis mainly concentrated in immune regulation and immune enhancement, removing chloasma, anemia improvment, anti-fatigue, relaxing bowel, improving sleep and anti-aging; and the compatibility of herbs with Angelica sinensis most often were Radix astragali membranacei, Radix rehmanniae, Poria cocos, Radix panacis ginseng, Radix codonopsitis pilosulae, Fructus zizyphi jujubae, Fructus lycii chinesis, Radix rehmanniae, Radix dioscoreae oppositae, Radix albus paeoniae lactiflorae, Colla Corii Asini, Saffron crocus, although compatibility is different, but the quality indexes are similar, the main detection of total polysaccharide and total saponin and total flavonoids. Research and development of health food containing Angelica sinensis should be in the form of regular food and adopt a multi index quality control model in future.
ABSTRACT
Objective The aim of this study was to establish a rat model of irritable bowel syndrome.Methods Thirty healthy adult SD rats ( mele:female=1:1 ) were divided into normal control group, model group, and positive control group ( pinaverium bromide tablets 15.0 mg/kg) for 31 days.Body weight, appetite, defecation, voluntary move-ment of all the rats were determined.The rates of gastric emptying and small intestinal propulsion rate were measured.The serum 5-HT and plasma SP and VIP or 5-HT, SP, VIP in colon homogenates were assessed by radioimmunoassay.Blood biochemical parameters were measured with an automatic biochemical analyzer.The gastric and intestinal morphology was evaluated by histological examination.Results After modeling, the rat weight and food intake were decreased, ad stool quantity was increased.The voluntary movement and gastric emptying rates were decreased, intestinal propulsion rates were increased, and the contents of SP and VIP in blood were decreased, but increased in the colonic homogenate (P＜0.05, P＜0.01).After treatment, the food intake was increased and stool quantity was decreased, the rat body weight was signifi-cantly increased, the amount of voluntary movement and stool returned near to normal, the 5-HT levels in serum or in co-lonic homogenate were significantly decreased, but plasma VIP levels were markedly increased, and the SP and VIP con-tents were significantly decreased in colonic homogenate in the positive control group (P＜0.05, P＜0.01).Hematology indexes had no obvious changes.The gastric and colonic tissue morphology showed no distinct damages caused by the di-verse stimulating factors.Conclusions The stimulation of composite factors can be used to successfully generate the rat model of irritable bowel syndrome, showing similar clinical manifestation of this disease in humans.
ABSTRACT
Objective To determine the content of ferulic acid, water and extract of Angelica formula particles, provide a reliable method for evaluation and quality control, and compare the quality of Angelica formula particles and Angelica pieces. Methods HPLC method was used with Kromasil C18 column (4.6 mm×250 mm, 5μm), flow rate of 1.0 mL/min, detection wavelength of 316 nm, column temperature at 28 ℃. The mobile phase was acetonitrile-0.05%phosphoric acid in water gradient elution system. Results Ferulic acid showed good linear relationship in the range of 0.033-0.165 μg (r=0.999 8). The average recovery was 98.78% and RSD=0.99%. Angelica formula particles met the quality requirement in the content indicators, the content of ferulic acid was higher than Angelica pieces. Conclusion The HPLC method for determination of ferulic acid in Angelica formula particles is simple and reliable, with good reproducibility, and can be used as the quality control method of Angelica formula particles.