ABSTRACT
Objective To investigate the effects of extract of Ginkgo biloba leaves EGb761 on 1-methy-l 4-phenylpyridium (MPP+)-induced injury in human neuroblastoma SH-SY5Y cells; To discuss its mechanism of action.Methods Cell culture method was used and SH-SY5Y cell damage model was induced with different concentrations of MPP+ to build Parkinson’s disease model in vitro. The experiment was divided into control group, MPP+ model group, low-, medium-, and high-dose EGb761 groups. The survival rate was determined by MTT assay, and the apoptotic rate was detected by flow cytometry according to AnnexinV apoptosis detection kit. The cell morphology was observed by inverted microscope. NO content in SH-SY5Y cells was detected by Nitric acid reduction method.Results Compared with the control group, the survival rate of SH-SY5Y cells decreased and the apoptotic rate and NO content increased in the model group (P<0.05); Compared with the model group, the survival rate of SH-SY5Y cells increased and the apoptotic rate and NO content decreased in the low-, medium- and high-dose EGb761 groups (P<0.05).Conclusion EGb761 can protect MPP+-induced SH-SY5Y cell from damage by the inhibition of the content of NO free radical.
ABSTRACT
OBJECTIVE To study the dynamics of nosocomial infections(NI) in our hospital in 2005 and to offer basis for reducing NI by surveillance.METHODS Data of internet report in 2005 were investigated.RESULTS The infection rate of NI in 2005 was 4.27%.The respiratory tract ranked the first place(75.2%);NI occurred mainly in Department of Medicine No.2,cadre ward and infection department;totally 85 isolates of bacteria were found,of which Gram-negative ones accounted for 51.76%.CONCLUSIONS The NI rate is connected with following factors such as age,underlying diseases,days in hospital,and the abuse of antibotics.