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Objective:To investigate the serum levels, diagnosis and prognosis value of endothelin-1(ET-1) in children suffering from lung metastasis of osteosarcoma.Methods:A total of 84 children with osteosarcoma, 67 children with lung metastasis of osteosarcoma and 35 healthy people from January 1, 2013 to January 1, 2018 in Second People′s Hospital of Nanyang were retrospectively included.The serum level of ET-1 was measured by performing enzyme linked immunosorbent assay(ELISA) methods and the influencing factors of serum ET-1 levels in children with lung metastasis of osteosarcoma were conducted by Logistic regression analysis.The clinical value of ET-1 in the prediction of the incidence of lung metastasis in children with osteosarcoma was analyzed by receiver operating characteristic (ROC) curve.Forty-five children with lung metastasis of osteosarcoma were followed up for 18 months and the prognosis value of serum ET-1 levels in children with lung metastasis of osteosarcoma was evaluated by Kaplan-Meier survival analysis. Results:The serum ET-1 level in children with lung metastasis of osteosarcoma was 97.23 (65.13, 134.98) ng/L and significantly higher than osteosarcoma group 60.21 (43.12, 74.63) ng/L and healthy control group 34.45 (12.01, 63.03) ng/L, respectively ( Z=-5.671, -4.92, all P<0.05), with significant differences. Logistic regression analysis proved that lung bilateral involvement ( OR=3.449), numbers of lung metastases (more than 3)( OR=3.449), average diameter of lung metastases (more than 5 cm) ( OR=6.501) and extrapulmonary metastasis ( OR=4.369) were independent risk factors for elevated serum ET-1 levels in children developing lung metastasis of osteosarcoma.The predictive value of ET-1 in the incidence of lung metastasis in children with osteosarcoma was significant (area under ROC curve: 0.841). When the cut-off value was 94.27 ng/L, the sensitivity and specificity were 88.5% and 92.6%, respectively.Survival analysis revealed that higher levels of ET-1 was correlated with poor prognosis ( OR=3.287, 95% CI: 1.119-9.547). Conclusions:The serum levels of ET-1 in children with lung metastasis of osteosarcoma are significantly elevated.ET-1 is a serological marker for the differential diagnosis of lung metastasis of osteosarcoma.Moreover, the higher levels of ET-1 are correlated with poor prognosis in children with lung metastasis of osteosarcoma.
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Objective:To systematically evaluate the effects of body mass index (BMI) on the prognosis of patients with diffuse large B-cell lymphoma (DLBCL).Methods:The databases (PubMed, Medline,Web of Science) were searched to identify clinical literatures about the relationship between BMI and the prognosis of DLBCL according to the inclusion and exclusion criteria. RevMan 5.3 software was used to analyze hazard ratio ( HR) and 95% confidence interval (95% CI) of overall survival (OS) and progression-free survival (PFS); the quality of included literatures, publication bias risk and heterogeneity were also evaluated. Results:There were 12 articles included in our research. Meta-analysis result showed that compared with normal weight patients (BMI 18.5-24.9 kg/m 2), the overweight patients (BMI 25.0-29.9 kg/m 2) had longer OS and PFS time, while the differences were not statistically significant (OS: HR=0.93, 95% CI 0.78-1.11, P=0.42; PFS: HR=0.89, 95% CI 0.67-1.20, P=0.45). The under-weight (BMI < 18.5 kg/m 2) (OS: HR=1.97, 95% CI 1.41-2.74, P < 0.01; PFS: HR=1.89, 95% CI 1.19-3.03, P < 0.01) and the obesity (BMI≥30.0 kg/m 2) patients both had shorter OS and PFS time, but the latter had no statistically significant difference (OS: HR=1.15, 95% CI 0.88-1.51, P=0.31; PFS: HR=1.32, 95% CI 0.90-1.94, P=0.15). No publication bias was observed in the symmetric funnel plot analysis. Conclusion:The increased BMI within a certain range is a protective factor for the prognosis of DLBCL patients.
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Objective:To explore the clinical characteristics, treatment and prognosis of neonatal septicemia caused by Streptococcus agalactiae.Methods:There were 29 cases of neonatal septicemia caused by Streptococcus agalactiae from January 2017 to December 2019 treated at Qingdao Women and Children′s Hospital.These cases were divided into the early-onset group (onset day of the patients ≤7 d) and late-onset group (onset day of the patients>7 d). Clinical data were collected for analysis.Results:The cases included in the current study accounted for 10.8% of all neonatal septicaemia during the same period.There were 23 cases born via vaginal delivery (79.3%) and 6 cases born by cesarean section (20.7%). Eighteen cases (62.1%) had early-onset (≤7 d) sepsis, and eleven cases (37.9%) had late-onset (>7 d) sepsis.The incidence of pneumonia was higher in the early-onset group compared with the late-onset group (83.3% vs.36.4%), while the incidence of purulent meningitis in early-onset group was lower than that in the late-onset group (33.3% vs.81.8%), and the length of hospital stay was shorter in early-onset group[(16.17±9.33)d vs.(36.73±27.43)d]. The differences between two groups were statistically significant (all P<0.05). Peripheral white blood cell counts elevated in 9 (31.0%) cases, and reduced in 15 (51.7%) cases.The levels of C-reactive protein increased in 18 (62.1%) cases, while the early value of procalcitonin increased in all cases.Drug sensitivity test showed that the sensitivity rates to erythromycin, clindamycin and tetracycline were 30.0%, 31.0%, and 24.1%, respectively.All strains were sensitive to penicillin G, ampicillin, vancomycin, linezolid and cefotaxime.A total of 28 cases were treated with two antibiotics.Twenty-two cases (75.9%) were cured, three cases (10.3%) were discharged after improvement, four cases (13.8%) died after abandoning treatment, and one case was left with neurological sequelae. Conclusion:Streptococcus agalactiae is one of the common pathogens of neonatal septicemia.Children with different onset time may have different complications.Decrease in white blood cell counts and elevation of procalcitonin are highly sensitive to neonatal septicaemia caused by Streptococcus agalactiae.Penicillin and/or cephalosporin antibiotics are the first choice in treatment, and the critically ill patients are treated with combination of two antibiotics.
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Objective To investigate the effect and application value of HC visual laryngoscope used for the emergency tracheal intubation on obese patients in the Emergency Department. Methods Totally 80 obese patients enrolled from January 2014 to December 2016 from Emergency Department, Second affiliated hospital of Xi'an Jiaotong University who needed the emergency tracheal intubation were randomly (random number) divided into two groups. Patients in group T were operated with traditional laryngoscope, and patients in group HC with HC visual laryngoscope. Then the success rate of glottis exposure, the trial times, operative time, success rate and complication rate of tracheal intubation were compared between the two groups. Results The success rate of glottis exposure in group HC was significantly higher than that in group T (95％ vs 77.5％, P<0.05). The one-time success rate of tracheal intubation and the total success rate of tracheal intubation in group HC were significantly higher than those in group T (72.5％ vs 37.5％, and 95％ vs 62.5％, respectively, P<0.05). However, the trial times of successful intubation cases and the operative time of successful intubation cases were significantly less than those in group T (1.26±0.40) vs (1.64±0.82), and (30.74±6.17) s vs (44.2±7.68) s, respectively, P<0.05. The complication rate of tracheal intubation in group HC was significantly less than that in group T (12.5％ vs 35％, P<0.05). Conclusions HC visual laryngoscope used for the obese patients in Emergency Department can not only increase the success rate of glottis exposure, decrease trial times and shorten operative time of intubation, but also improve the success rate and decrease the complication rate of emergency tracheal intubation, thus having a certain application value.
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Objective To understand the distribution condition of resources of medicinal plant of Euodiae Fructus;To provide references for resource conservation and standardized plantation of Euodiae Fructus. Methods Literature search, interview survey and field survey were conducted to investigate the resource conditions of the main producing areas of Euodiae Fructus. Samples were collected and TKW of the medicinal plant was weighted. Results Currently, the main producing areas of Euodiae Fructus are provinces of Jiangxi, Guizhou, Hubei, Hunan and part of provinces and places of Guangxi, Anhui, Zhejiang, Shanxi, and Chongqing. Commercial Euodiae Fructus mainly comes from private investors. The wild resources of Euodiae Fructus are few. The TKW of the medicinal plant is within 5.34-45.62 g, with great differences. The national annual output of cultivated Euodiae Fructus is around 3500 t. The production and marketing is in balance. The price for Euodiae Fructus is relatively stable. Conclusion The plantation of Euodiae Fructus is dispersing, with small scale, low degree of standardization and intensification, and great quality differences. Standardized, large-scale, and industrialized plantation base should be established, and the assessment system for medicine quality should be perfected, with a purpose to ensure the qualified and stable production of Euodiae Fructus. Meanwhile, wild resource production should be enhanced to maintain the biological diversity and promote the sustainable development of Euodiae Fructus.
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Objective To investigate the effects of extended nursing based on relationship, information and management on the self-care behaviors of patients with heart valve replacement. Methods According to admission time, 37 patients with heart valve replacement were set as the control group, which was given routine nursing intervention and another 42 patients as the study group, which received extended nursing based on the trinity of relationship, information and management 2~8 weeks after discharge. Before and after intervention, patients were evaluated by using the improved version of self-care behavior evaluation scale of heart disease (SNBAOHD). Result There were significant differences between the two groups in other items of SNBAOHD, except two items of low salt diet and medication (P<0.05). Conclusions Extended nursing after discharge based on the trinity of relationship, information and management can help the patients to be continuously cared. In this case, it is effective for the enhancement of off-hospital self-care ability and prevention of disease relapses.
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Objective To investigate the clinical and laboratory feature of neonatal hemolytic disease (HDN)occurred in Qingdao caused by mother-baby ABO blood type disagreement.Methods Serum bilirubin (TBIL)test and micro column gel technology were used on 422 cases neonatal hemolytic disease children blood samples (collected from Jun.2013 to Feb.2015).Results There were 388 cases first-born children among 422 cases including 206 cases of type A and 216 of type B and the difference between them was not statistically significant (χ2 =0.24,P >0.05).The difference between male (218 cases)and female (204 cases)was not statistically significant (χ2 =0.24,P >0.05)too.The indirect bilirubin (IBI)increas-ing was more obviously.The peak level of serum bilirubin was 116~465 μmol/L and 256.5~342.0 μmol/L was 38.9% (χ2=0.24,P >0.05).162 cases reticulocyte count was increased nearly 38.5% (χ2 =75.62,P <0.05).RBC antibody release test and serum free antibody test were often positive and the percentage was 80.1% (χ2 =146.98,P <0.05).Conclusion The neonatal hemolytic disease may turn up in first-born children.The child with three positive test was more sensitive to neonatal hyperbilirubinemia.RBC antibody release test and serum free antibody test are often positive.It is important to make the early diagnosis and treatment as soon as possible for reducing the bilirubin encephalopathy.
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Objective To evaluate the effect of pituitrin and nursing for patients with vasoplegic syndrome after cardiopulmonary bypass. Methods 38 patients with cardiopulmonary bypass after open heart operation were divided into two groups with 19 cases in each group according to odd-nunbered and even-numbered days, the PT group was given pituitrin treatment, the NE group was given norepinephrine treatment. Heart rate, MAP, urine volume were recorded at 0.5, 1, 2, 6, 12 and 24 hours after drug therapy.Mechanical ventilation time, ICU stay time,hepatic and renal function and clinical symptom were compared between the two groups. Results MAP and urine volume increased significantly at 0.5 hour after pituitrin therapy. MAP increased from (55.33±4.10)mmHg to (67.34±4.13)mmHg. Urine volume increased from (0.08±0.02)ml·kg-1·h-1 to (1.84±1.21)ml·kg-1 ·h-1 MAP and urine volume also increased at other time points. In the NE group, MAP increased at 1h after treatment. Both MAP and urine volume increased at 2h and later after norepinephrine treatment. One death happened in the NE group. Compared with the NE group, the PT group was associated with lower ICU stay, mechanical ventilation time and 24h serum LAC.Liver and kidney function, myocardial infarction, nausea and vomiting, and other side effects were not different between the two groups. Conclusions Application of low-dosage pituitrin is rapid, efficient and safe treatment for patients with vasoplegic syndrome after open heart operation.
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To explore the protective effect of adenovirus mediated IGF-Ⅰgene(Ad-IGF-Ⅰ)transfer on non-obese diabetic(NOD)mice. The results showed that the incidence of diabetes and degree of insulitis were significantly reduced in mice receiving Ad-IGF- Ⅰ . Treatment with Ad-IGF- Ⅰ significantly decreased apoptosis rate,expression of Fas and caspase-3, and increased expression of Bcl-xl. This study indicates the potential of IGF- Ⅰ gene therapy in protecting NOD mice from insulitis and apoptosis.
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<p><b>OBJECTIVE</b>To study HPLC characteristic fingerprint of Moghania philippinensis and provide a reliable method for scientific evaluation and quality control.</p><p><b>METHOD</b>The chromatographic separation was performed on a Kromasil C15 column (4.6 mm x 250 mm, 5 microm). The mobile phase consisted of acetonitrile and water containing 0.3% acetic acid in gradient elution. The column temperature was set at 40 degrees C and the detection wavelength was at 254 nm.</p><p><b>RESULT</b>The developed HPLC fingerprint method above was applied to analyze 23 batches of crude herbs of Moghania philippinensis. The total 22 peaks were selected as the characteristic peaks. And five of them were identified as genistein, genistin, 5,7,3',4'-tetrahydroxy-6,8-diprenylisoflavone, 5,7,4'-trihydroxy-6,8-diprenylisoflavone and flemiphilippinin E by matching their retention time, UV spectra data with those of the authentic chemical reference substances.</p><p><b>CONCLUSION</b>The method is simple, accurate and reproducible. It is suitable for the quality control of M. philippinensis.</p>
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Chromatography, High Pressure Liquid , Methods , Drugs, Chinese Herbal , Chemistry , Fabaceae , Chemistry , Plant Roots , Chemistry , Plants, Medicinal , Chemistry , Reproducibility of ResultsABSTRACT
To study the chemical constituents of the stems of Clematis parviloba, six compounds were isolated from a 95% ethanol extract by using a combination of various chromatographic techniques including column chromatography over silica gel, ODS, Sephadex LH-20, and semi-preparative HPLC. Two new phenolic glycosides, 2-((E)-3-carboxybut-2-en-yl)-4-hydroxy-3-methyl-phenyl-O-beta-D-glucopyranoside (1) and 4'-hydroxy-phenol-beta-D-[6-O-(4"-hydroxy-3", 5"-dimethoxy-benzoate)] glucopyranoside (2) were isolated, together with a known phenolic glycoside, 4'-hydroxy-3'-methoxy-phenol-beta-D-[6-O-(4"-hydroxy-3", 5"-dimethoxy-benzoate)] glucopyranoside (3) as well as three known megastigmane glycosides, linarionoside A (4), linarionoside C (5), and staphylionoside K (6). Their structures were determined on the basis of spectroscopic analysis and chemical evidence. Among them, compounds 1 and 2 were named as clemaparvilosides A (1) and B (2), respectively, and compounds 3-6 were obtained from Clematis genus for the first time.
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<p><b>OBJECTIVE</b>To develop a Quantitative Assay of Multi-components by Single - marker (QAMS) for simultaneous determination of three components in Fructus Evodiae, and examine the feasibility of using the relative correction factors between the different types of compounds.</p><p><b>METHOD</b>Rutaecarpine was selected as the internal reference substance; the relative correction factors of evodin and evodiamine were calculated. The contents of three components in 11 batches of samples were determined by both external standard method and QAMS. The validity of the QAMS method was evaluated by comparison of their quantitative results.</p><p><b>RESULT</b>No obvious differences (RSD < 5%) were found in the quantitative results of evodin and evodiamine in 11 batches of Fructus Evodiae determined by the two methods.</p><p><b>CONCLUSION</b>It is feasible and suitable to determine evodin and evodiamine in Fructus Evodiae by QAMS, and this method can be used for a certain different types of compounds.</p>
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Chromatography, High Pressure Liquid , Methods , Drugs, Chinese Herbal , Evodia , Chemistry , Furans , Heterocyclic Compounds, 4 or More Rings , Indole Alkaloids , Plant Extracts , QuinazolinesABSTRACT
<p><b>OBJECTIVE</b>The aim of this study was to investigate the features of mast cell subtypes and relationship between mast cells and pathogenesis of oral lichen planus.</p><p><b>METHODS</b>Mast cells in the tissue of oral lichen planus were stained with Alcian blue/safranine in order to demonstrate mature and immature mast cells, and the constituent ratio of these two subtypes was calculated. Afterwards, LUZEX-F image pattern analysis system was applied to demonstrate the size of these two subtypes and their morphological features.</p><p><b>RESULTS</b>The constituent ratio of mature and immature types of mast cells in the tissue of oral lichen planus was significantly different from that in the normal tissue (P < 0.01), and such difference was also demonstrated among different types of this malady, including papule-, net- and erosion-type of oral lichen planus (P < 0.05). There was significant difference between the sizes of mature and immature mast cells (P < 0.01) and also the sizes, amount and distribution of the plasma granules within these two mast cells.</p><p><b>CONCLUSION</b>It could be implied that mast cells might play a role in the pathogenesis of oral lichen planus.</p>