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Objective:To study the effect of ligustrazine on the proliferation and collagen production of human embryonic lung fibroblasts MRC-5.Methods:MRC-5 was cultured and divided into control group, low-dose group, medium-dose group and high-dose group. The control group was treated with DMEM without drugs, while the low-dose group, medium-dose group and high-dose group were treated with DMEM with different doses of ligustrazine. After 24, 36 and 48 h treatment, the cell proliferation activity was detected by MTS assay; the cell cycle was detect by flow cytometry; the apoptosis was measured by TUNEL staining; Western blot was used to detect the expression of Bcl-2, Bax, CyclinD1, P27 protein, and ELISA was used to detect the levels of TGF-β1, Col-Ⅰ and Col-Ⅲ.Results:After 24, 36 and 48 h treatment, compared to the control group, the proliferation inhibitory rate of low-, medium-, and high-dose group increased significantly ( P<0.05). After 48 h treatment, compared to the control group, the G0/G1 phase proportion of cell cycle in different doses of ligustrazine group significantly increased, and the S phase proportion of cell cycle in different doses of ligustrazine group significantly decreased ( P<0.05). Compared to the control group, the apoptosis rate (6.59% ± 0.95%, 10.92% ± 2.25%, 16.58% ± 3.25% vs. 1.38% ± 0.34%) in different doses of ligustrazine group significantly increased ( P<0.05). Compared to the control group, the expression of Bcl-2 (0.79 ± 0.13, 0.52 ± 0.06, 0.31 ± 0.05 vs. 0.91 ± 0.15) and CyclinD1 (0.62 ± 0.08, 0.50 ± 0.06, 0.27 ± 0.04 vs. 0.83 ± 0.14) in different doses of ligustrazine group significantly decreased, while the expression of Bax (0.45 ± 0.07, 0.50 ± 0.06, 0.79 ± 0.13 vs. 0.32 ± 0.06) and p27 (0.39 ± 0.07, 0.75 ± 0.13, 0.96 ± 0.16 vs. 0.20 ± 0.05) significantly increased ( P<0.05). The content of TGF-β1, Col-Ⅰ and Col-Ⅲ in different doses of ligustrazine group were significantly decreased ( P<0.05). Conclusions:Ligustrazine can inhibit the proliferation and collagen production of human embryonic lung fibroblasts, which may be related to the induction of cell cycle arrest, regulation of proliferation and cell cycle related proteins expression.
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This study was aimed to analyze and summarize the intervention rule on traditional Chinese medicine (TCM) external treatment of primary dysmenorrhea,in order to provide theoretical evidences for clinical treatment.Articles on TCM external treatment of primary dysmenorrhea published both at home and abroad were search in the CNKI from January Ft,2014 to January Ft,2017.After standard screening,the frequency analysis,cluster analysis and association analysis were conducted.The results showed that acupuncture was the mostly used TCM external treatment of primary dysmenorrhea,which was far more than other types of TCM external treatments.Theoretical studies on single usie of acupuncture or single use of moxibustion were far more than clinical trials.The acupoint used with the highest frequency in primary dysmenorrhea treatment was SP-6 Sanyinjiao.RN-4 Guanyuan was the second and SP-8 Diji was the third.A total of 22 classic acupoint combinations were identified.It was concluded that the main external intervention method of primary dysmenorrhea was acupuncture with SP-6,RN-4 and SP-8 as its main acupoints.There are a total of 22 classic acupoint combinations
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This study was aimed to explore the medication rules of Chinese herbal medicine compound in the treatment of primary dysmenorrheal based on data mining.Relevant literatures on Chinese herbal medicine compound in the treatment of primary dysmenorrhea were retrieved from June 2006 to June 2016 in the Chinese Journal Full-text Database (CNKI) to establish a literature database.The Excel 2010,SPSS 22.0 and SPSS Modeler 14.2 software were used in the frequency analysis,cluster analysis,association analysis,and etc.The results showed that literatures related to Chinese herbal medicine compound in the treatment of primary dysmenorrhea,there were 74 compound formula and 76 kinds of Chinese herbal medicine involved.Angelica was the core single Chinese herbal medicine.Chinese herbal medicine with high-frequency usage was the type of removing blood stasis,invigorating qi and blood.Cluster analysis can form 5 clustering combination.Primary dysmenorrhea was mainly clustered to two syndromes,which were qi and blood deficiency with blood stasis,liver and kidney deficiency with qi stagnation and blood stasis.The association rules analysis showed that there were a total of 25 rules,with the most common combination of two or multiple combination from four kinds of Chinese herbal medicine,including Angelica,Radix Paeoniae Alba,Rhizoma Chuanxiong,and Rhizoma Corydalis.It was concluded that blood stasis was the direct cause of primary dysmenorrhea,many of which belonged to the deficiency of root (i.e.,deficiency of qi,blood and cold deficiency) and excess of branch.Chinese herbal medicine treatment should be focused on the regulation of both qi and blood,invigoration of both liver and kidney,as well as harmonization of the blood.
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This study was aimed to summarize and analyze acupoint selection rule of acupuncture in the treatment of primary dysmenorrhea in recent years,and to guide acupoint selection and compatibility in clinical practice.Relevant literatures in recent years were retrieved from the China National Knowledge Infrastructure (CNKI) full-text database.And then,literatures involving acupuncture prescriptions were summarized.Data were integrated and input.Finally,the SPSS software was combined to analyze the data of acupoint selection in the acupuncture prescription to achieve the conclusion.The results showed that in the acupuncture treatment of primary dysmenorrhea,the main meridian selections were the Ren Meridian and the Spleen Meridian of Foot Taiyin.The generally used acupoints were SP6-Sanyinjiao,RN4-Guanyuan,SPS-Diji,and etc.The multi-point compatibility therapy was used.It was concluded that although the advantages of acupuncture in the treatment of primary dysmenorrhea are prominent,the pathogenesis is mainly related to the liver,spleen and kidney,which are recognized by most physicians.However,there are still large differences in acupoint compatibility and acupoint selection.Hence,there are still no unified,scientific and standardized diagnosis and treatment standards.Further exploration should be made to guide clinical practice.
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Through consulting related literatures on modern medicine pathogenesis and treatment status of chronic pain,new clinical treatment methods were revealed and summarized.Literatures on modern medicine pathogenesis and treatment of chronic pain were retrieved from the CNKI,Wanfang Data,VIP,and Pubmed database from January 2000 to December 2016.Literatures with big influence,strong representation and new treatment method were screened.All methods were classified to summarize the pathogenesis and clinical application situation.This paper discussed the pathogenesis of peripheral sensitization,central sensitization,psychological mechanism and clinical medication.Through nearly 15 years of literature analysis,the pathogenesis of modern medicine and treatment status of chronic pain were summarized.
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<p><b>OBJECTIVE</b>To study retrospectively the clinical efficacy on pediatric recurrent pneumonia treated with point application in summer for the prevention in winter, as well as the relationship of age, sick duration, attack frequency and skin reaction with the clinical efficacy.</p><p><b>METHODS</b>One hundred and thirty-five cases of pediatric recurrent pneumonia were divided into a one-year group, a two-year group and a three-year group, 45 cases in each one according to the duration of treatment. The acupoints for the application were Dingchuan (EX-B1), Feishu (BL 13), Gaohuang (BL 43) and Danzhong (CV 17) with the same herbal plaster (prepared with rhizome corydalis, semen brassicae, euphorbia kansui and asarum sieboldii at the ratio of 2:2:1:1) on the first day of each of the three periods of the hot season, 2 to 4 h in each treatment. The attack frequency and change rate were observed before and after treatment in the three groups. The clinical efficacy was assessed in the three groups.</p><p><b>RESULTS</b>(1) After treatment, the attack frequency of pediatric pneumonia was reduced apparently in the three groups (all P < 0.01). The result in the three-year group was less than that in the one-year group and the two-year group and the change rate was the highest (all P < 0.01). (2) After treatment, the sick duration was shortened apparently in the three groups (P < 0.05, P < 0.01). The result in the three-year group was the most remarkable, statistically and significantly different as compared with the other two groups (both P < 0.01). (3) The total effective rate in the three-year group was better than that in either of the other two groups [84.4% (35/45) vs 51.1% (23/45, P < 0.01), 84.4% (35/45) vs 71.1% (32/45, P < 0.05)]. (4) The total effective rate in the children aged from 4 to 7 years was better than that in the group aged from 8 to 10 years and the group aged from 11 to 14 years [79. 7% (47/59) vs 71.7% (33/46, P < 0.05); 79.7% (47/59) vs 43.3% (13/30, P < 0.01)]. (5) The total effective rate in the children with the sick duration ≥ 4 year was lower than that in the group with the sick duration <2 years and that 2 to 4 years (both P < 0. 01). (6) The total effective rate in the children with the annual attack frequency of 2 to 4 times was better than that with the frequency ≥ 4 times (P < 0.01). (7) For the cases with skin reaction after treatment, the total effective rate was better than that in the cases without reaction (P < 0.05).</p><p><b>CONCLUSION</b>The point application in summer for the prevention in winter reduces the attack frequency of pediatric pneumonia, shortens the sick duration and has achieved the better significant efficacy in the cases of lower age, shorter sick duration, less attack frequency and moderate skin reaction.</p>
Subject(s)
Adolescent , Child , Child, Preschool , Female , Humans , Male , Acupuncture Points , Drugs, Chinese Herbal , Pneumonia , Drug Therapy , Seasons , Treatment OutcomeABSTRACT
This study was aimed to discuss the anti-inflammatory and immunization properties ofRe-Du-Ning (RDN) injection in the treatment of acute exacerbation of chronic obstructive pulmonary disease (AECOPD) with phlegm-heat stagnated in the lung syndrome. A total of 110 in-patients were collected from January 2012 to December 2013. Cases were randomly divided into the treatment group (basic treatment plan + 20 ml RDN injection + 250 mL of 5% GS injection, once a day, intravenous injection) and the control group (basic treatment plan + 20 ml of 0.9% NS injection + 250 mL of 5% GS injection, once a day, intravenous injection), with 55 cases in each group. The treatment course was 14 days. Observations were made on traditional Chinese medicine (TCM) symptom score, clinical effect, blood routine examination, blood gas analysis and T lymphocyte subgroups before and after the treatment in both groups. The results showed that in the aspects of TCM symptom score and clinical effect, the posttreatment TCM symptom score and total integration in the treatment group were obviously improved compared to the control group except for shortness of breath, dry mouth and thirsty, flushing (P < 0.05). The total effective rate was obviously better than that of the control group. In the aspect of anti-inflammation, the total white blood cell (WBC) count and the normal neutrophil percentage of both pretreatment and posttreatment in the treatment group were obviously decreased compared to pretreatment (P < 0.05). However, there were no statistical differences on WBC count and normal neutrophil percentage compared to posttreatment in the control group. In the aspect of blood gas analysis, the posttreatment PaCO2 and PaO2 of the treatment group were obviously better than the control group (P < 0.05). In the aspect of immune regulation, the posttreatment T lymphocyte subgroups CD3+ and CD4+ of the treatment group were higher than the control group. The expression inhibition / cytotoxic lymphocyte (CD8+) was obviously lower than the control group (P < 0.05). The CD4+ / CD8+ was back to the normal reference level. It was concluded that in the treatment of AECOPD with phlegm-heat stagnated in the lung syndrome, on the basis of western medicine symptomatic treatment plan, RDN injection was assisted to clear heat, relieve toxin and remove phlegm. It can obviously improve patient’s clinical symptoms and increase the clinical therapeutic effects. The treatment was especially targeted to infection-induced respiratory failure patients combined low immunity with possible identified therapeutic effects.
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This article elaborated the theory of chronic and complex disease treatment based on collaterals. It put forward that complex virtual evil stasis was the common pathogenesis of chronic and complex disease treatment. Combined with articles published in recent 5 years, from the perspective of cytokines and genes, it summarized traditional Chinese medicine (TCM) compound targets in chronic and complex disease treatment. It put forward the transforming growth factor-β1 (TGF-β1), tumor necrosis factor-α(TNF-α), interleukin-6 (IL-6), vascular endothelial growth factors (VEGFs), matrix metalloproteinases/tissue inhibitor of metalloproteinase (MMPs/TIMPs) were its high-frequency targets. It provided a basis for future experiment design on chronic and complex disease treatment based on collaterals as well as new drug development.
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Idiopathic pulmonary fibrosis (IPF) is defined as a specific form of chronic and progressive fibrosing inter-stitial pneumonia. Till now, it is lack of effective and safe treatment. In recent years, more and more clinical trials of traditional Chinese medicine (TCM) have been conducted with different evaluation criteria and indexes. The knowl-edge is disunity and unsystematic. From the perspective of combination of disease and TCM syndrome model, this ar-ticle summarized and organized the current data and medical standards, and then come up with the new evaluation system of clinical effect and describe important indexes and methods. This article was aimed to improve the clinical trial design and provide high quality evidences for evidence-based medicine.
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Idiopathic pulmonary fibrosis(IPF), as one of the chronic and complex diseases, is with high incidence and mortality rate. It is also a difficult to treat disease in modern medicine. Traditional Chinese medicine (TCM) syndrome differentiation and treatment embodies characteristics and advantages. In recent years, the collateral disease theory guiding the prevention and treatment of the chronic and complex diseases has become a hot point and frontier in medical research, and achieved certain effects. This article is to summary the structures and functions of pulmonary collaterals based on the collateral disease theory. Combined with clinical manifestations of IPF, we put forward Fei-Re Luo-Y u as the fundamental role in the pathogenesis of IPF in its acute phase to lay the theoretical foundation to the further exploration of syndrome prevention and differentiation on IPF in its acute exacerbation.
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To observe the expressions of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in rats with pulmonary fibrosis (PF) induced by bleomycin, and to explore the mechanisms of Shenlong Decoction in preventing and treating PF.