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Objective:To evaluate the efficacy and safety of Xuebijing injection for treating sepsis by systematic review.Methods:Randomized controlled trials (RCT) of relevant Xuebijing injection for sepsis were searched in PubMed, Cochrane Library, CNKI, Wanfang database, VIP database from January 2000 to July 2019. Patients in control group were given conventional treatment, while patients in experimental group were treated with Xuebijing injection combined with conventional treatment. The qualities of the included trials were evaluated according to the Cochrane bias risk assessment tool. Meta-analysis of 28-day mortality, acute physiology and chronic health evaluation Ⅱ (APACHEⅡ), white blood cell count (WBC), C-reactive protein (CRP) and safety for sepsis patients with Xuebijing injection were conducted by RevMan 5.3 software. And the funnel chart was used to evaluate the publication bias of 28-day mortality.Results:Fourteen clinical studies involving 938 patients in total were enrolled, among whom 475 cases in experimental group and 463 cases in control group. Meta-analysis showed that compared with the control group, the 28-day mortality, APACHEⅡ score, WBC and CRP of the experimental group were significantly decreased [28-day mortality: relative risk ( RR) = 0.52, 95% confidence interval (95% CI) was 0.40 to 0.67, P < 0.000 01, I2 = 0%; APACHEⅡ score: mean difference ( MD) = -5.48, 95% CI was -7.52 to -3.43, P < 0.000 01, I2 = 86%; WBC: MD = -2.26, 95% CI was -3.35 to -1.17, P < 0.000 1, I2 = 87%; CRP: MD = -37.43, 95% CI was -56.70 to -18.16, P = 0.000 1, I2 = 99%]. None of the 14 literatures reported the safety of Xuebijing injection, and there were no reports of participants' withdrawnness in halfway. Funnel chart analysis showed that there was a publication bias among studies with 28-day mortality. Conclusion:Xuebijing injection can improve the clinical symptoms, significantly reduce the mortality, and has high clinical application value.
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Objective To establish the quality standards system of A system to multiple evaluation on Yinxing huonao capusle,and discuss the feasibility of the method whether A system to multiple evaluation to be used for traditional Chinese medicine quality standard.Methods Identification of the Yinxing huonao capusle by TLC method was established by one thin layer chromatography system.HPLC was used to detect 6 types of the primary active components in Yinxing huonao capusle.Results 5 types of the primary active components were synchronize identified by one thin layer system.The color of spots was clear and had a good separation effect.6 kinds of the primary active components were synchronize detected by one system with high accuracy.Conclusion The established method A system to multiple evaluation can be used for the study of the quality standards system of Yinxing huonao capusle.
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Objective To explore the extraction process of Yinxing Huonao capusle.Methods Central composite design-response surface method was used to optimize the extraction process with quantity of water and boiling time as main factors and with syringin transfer rate,emodin transfer rate and solids retention rate as evaluation index.Results The conditions of the optimized extraction process were as follows:10-fold water,extracting time of 160 min,extracting 2 times.Conclusion It is feasible that central composite design-response surface method is used to optimize the extraction process of Yinxing Huonao capusle,and the optimized extraction process is simple and reliable.
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OBJECTIVE:To establish the quality standard of Compound duzheng tablets. METHODS:TLC method was used for qualitative identification of Vitis davidii,Aralia chinensis,Acanthopanax senticosus and Panax notoginseng in the preparation. The contents of syringoside,oleanolic acid,ginsenoside Rg1,ginsenoside Rb1 and notoginsenoside R1 were determined by HPLC. The determination was performed on Inertsil ODS-SP C18 column with mobile phase consisted of acetonitrile-water(gradient elu-tion)at the flow rate of 1.0 mL/min. The detection wavelength was set at 215 nm,and the column temperature was 25 ℃. The sam-ple size was 10 μL. RESULTS:TLC spots of V. davidii,A. chinensis,A. senticosus and P. notoginseng were clear and well-separat-ed without interference from negative control. The linear ranges of syringoside,oleanolic acid,ginsenoside Rg1,ginsenoside Rb1 and notoginsenoside R1 were 0.056-0.337 μg(r=0.9998),0.022-0.130 μg(r=0.9999),0.030-0.182 μg(r=0.9995),0.010-0.061 μg(r=0.9998),0.038-0.226 μg(r=0.9999),respectively. RSDs of precision,stability and reproducibility tests were all lower than 3.0%. The recoveries were 100.55%-103.80%(RSD=2.0%,n=9),100.00%-104.56%(RSD=3.0%,n=9),97.06%-102.94%(RSD=2.1%,n=9)、97.06%-102.94%(RSD=2.0%,n=9),96.67%-100.00%(RSD=1.5%,n=9). CONCLUSIONS:Established standard can be used for the quality control of Compound duzheng tablets.