ABSTRACT
<p><b>OBJECTIVE</b>To study the influence of saikosaponins on function of rats' liver mitochondria, its liver damage mechanism was discussed.</p><p><b>METHOD</b>Administrating alcohol eluent of saikosaponins of different dose for 15 days to rats, and the high, middle and low lose-group are separately 300, 150, 50 mg x kg(-1) caculated by total saikosaponins. The liver index in serum, the respiratory function of liver mitochondria,the content of ATP and the activity of ATP enzyme were detected. The weight of heart, liver, spleen, lung, renal of rats were precisionly weighed, and the ratio of organ to body were calculated. The histopathologic examination of hepatic tissue were examined.</p><p><b>RESULT</b>Alcohol eluent of saikosaponins of different dose can induce apparent decrease of PCR, P/O value, respiratory oxygen consumption and the activity of ATP enzyme; the level of ALT, AST and ALB in serum increased; the liver weight and the ratio of liver to body increaseed, and the hepatic tissue damage is obvious in the histopathologic examination of hepatic tissue. The above-mentioned changes gradually aggravates with dose increasing, and it is obviously discrepancy compared with control group.</p><p><b>CONCLUSION</b>Alcohol eluent of saikosaponins can induce liver damage by restraining the respiratory function of mitochondria and effecting liver's energy metabolism. Other hepatoxicity mechanism still need to be discussed.</p>
Subject(s)
Animals , Female , Male , Rats , Antimetabolites , Toxicity , Chemical and Drug Induced Liver Injury , Metabolism , Pathology , Disease Models, Animal , Dose-Response Relationship, Drug , Electron Transport , Energy Metabolism , Liver , Pathology , Mitochondria, Liver , Metabolism , Oleanolic Acid , Toxicity , Organ Size , Saponins , ToxicityABSTRACT
<p><b>OBJECTIVE</b>To explore the mechanism of glutamate (Glu)-induced PC12 cell apoptosis and the protective effect of muscone.</p><p><b>METHOD</b>PC12 cells were randomly divided into six groups: normal group (Normal), model group injured by glutamate (Glu), nimodipine group (Nim) and muskone groups (Mus) of high, middle and low doses. The PC12 cells were pretreated with or without different concentrations of muskone for 30 min and then exposed to glutamate. MTT assay for cell survival, flow cytometric detection of apoptotic cells, DCF assay for reactive oxygen species (ROS) and flow cytometric assay was performed to determine the mitochondrial membrane potential in PC12 cell.</p><p><b>RESULT</b>PC12 cell damage, the concentrations of [Ca2+], and apoptosis induced by Glu were decreased after being administrated with paeoniflorin.</p><p><b>CONCLUSION</b>Muskone inhibited Glu-induced apoptosis in PC12 cells. The mechanism is related to inhibiting intracellular Ca2+ overload and maintaining mitochondral membrance potential.</p>
Subject(s)
Animals , Rats , Apoptosis , Calcium , Metabolism , Cell Survival , Cycloparaffins , Pharmacology , Glutamic Acid , Pharmacology , Membrane Potential, Mitochondrial , PC12 Cells , Pheochromocytoma , Metabolism , Protective Agents , PharmacologyABSTRACT
<p><b>OBJECTIVE</b>To compare the influence of different Bupleurun chinense composition to the degree of hepatotoxicity damage to rats and oxidative damage mechanism.</p><p><b>METHOD</b>To successively lavage alcohol extracted and water extracted B. chinense composition to rats for 30 days, the general conditions were observed and the related index of liver function, the content of total-SH in serum, the content of MDA, the activity of SOD and the content and activity of GSH and GSH-Px in serum and liver tissue were detected.</p><p><b>RESULT</b>Alcohol and water extracted B. chinense composition all could induce the increases of the activity of ALT and AST in serum, liver weight and the ratio of liver to body, and the content of MDA and induce the decreses of the content of total -SH in serum, the content of GSH, and the activity of SOD and GSH-Px in serum and liver tissue. The above-mentioned changes gradually aggravated with dose increasing, and there was significant difference compared with control group with distilled water.</p><p><b>CONCLUSION</b>The different B. chinense composition all can induce hepatotoxicity damage, and the channel of hepatic damage is related with the peroxidative damage mechanism. The degree of hepatotoxicity damage caused by the alcohol extracted composition is more serious than that by the water extracted composition.</p>
Subject(s)
Animals , Female , Male , Rats , Bupleurum , Chemistry , Dehydroascorbic Acid , Metabolism , Liver , Metabolism , Oxidative Stress , Plant Extracts , Toxicity , Random Allocation , Rats, Wistar , Superoxide Dismutase , MetabolismABSTRACT
Objective To observe the effect of muscone on the blood-brain barrier and neuropathological changes after complete cerebral ischemia/reperfusion of rats.Methods The global cerebral ischemia/reperfusion injury model was established by the four-vessel occlusion(4VO) method,and then the model animals were given high-,middle-and low-dose muscone.Meanwhile,sham-operation group,model group,and positive control group were also set up.The rats neuroethology behavior,and the contents of superoxide dismutase(SOD),malondialdehyde(MDA) and excitatory amino acid(EAA) in ischemic brain tissue were examined.Results Muscone increased SOD content,lowered MDA content,reduced the increase of EAA content caused by ischemia and hypoxia,and inhibited the excitatory neurotoxicity caused by EAA.Conclusion Muscone has obvious protective effect on rats with complete cerebral ischemia/reperfusion injury.