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Objective To investigate the effect and the potential mechanisms of low molecular polysaccharide from agaricus blazei (LMPAB) on H2O2-induced oxidative injury in hippocampal neuronal cells of rats.Methods Hippocampal neuronal cells were isolated from SD rats (24 h) and grew in culture.Cultured cells were divided into normal control group (added the same amount of nutrient solution), model control group (added 500 μmol·L-1H2O2 solution) and LMPAB high, medium, low dose groups (added 20,10,5 mg·L-1 LMPAB solution, respectively, then added 500 μmol·L-1 H2O2 solution each).The hippocampal neuron cell activity was detected with MTT method.The hippocampus neuron mitochondrial membrane potential (MMP) was detected by flow cytometry.According to the reagent instruction methods, malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) activities were detected.Results The activities of cell, CAT, SOD, GSH-PX and MMP in normal control group and the LMPAB high dose group were significantly higher than those of model control group (P<0.01);The content of MDA in normal control group and LMPAB high dose group was significantly lower than that of model control group (P<0.01).Conclusion The protective effect of LMPAB on hippocampal neurons with H2O2-induced injury may be related with the mechanism of enhancing the neuronal antioxidative capacity.
ABSTRACT
Contents of laboratory diagnosis is complex.Traditional teaching method cannot arouse students' interest in study and the teaching effect is poor. Teachers should choose different teaching emphasis for students in different specialty,increase and decrease theory teaching contents according to the demands of students' future actual work,introduce case analysis,train students' capability of clinical logical thinking,enhance students' perceptual knowledge of theory through the training of experiment skills and using PBL teaching method to make students truly participate in teaching course so as to arouse their enthusiasm,These reforming measures will benefit the improvement of teaching quality.
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Objective This study was designed to investigate the expression and clinical significance of transcription factor E2F-1 and apoptosis-off gene Bcl-2 in human colorectal carcinoma.Methods Immunohistochemistry is used to detect the expression of E2F-1、Bcl-2 proteins in 60 cases of human colorectal carcinoma tissues and their corresponding normal mucosa.Results The positive expression rate of E2F in cancer tissues is 56.7%,while the positive expression rate of E2F in corresponding normal mucosa is 6.7%,so the comparison between two tissues is significant (P
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Objective To investigate the role of glutaredoxin1(Grx1)on high glucose-induced apoptosis in cultured umbilical vein endothelial cells.Methods Human umbilical vein endothelial cells was cultured and induced with different dose of glucose and Grx1.We divided the cells into three groups:cell control group,damage group(high glucose group),pretreatment with Grx1 group(Grx1+ high glucose group).The morphological changes of the cells were observed by light microscope.The proliferation of cell was measured by MTT assay.The morphon of cell nucleolus of endothelial cell was observed in a fluorescence microscope by Hoechst 33258 stain and the influences of Grx1 on the apoptosis were determined by the immunofluorescent of Annexin V-FITC/PI with flow cytometer.Results Under the light microscope Grx1 ameliorated cells condition and restore the structure of organelle compared with damage group.Grx1 prevented the inhibitory effect on cell viability induced by high glucose;Hoechst33258 stains suggested Grx1 protect the cells nucleolus against high glucose-induced apoptosis.The analysis of Grx1 can restrain apoptosis rate of endothelial cell significantly.Conclusion Grx1 can obviously protect human umbilicus vein endothelial cells from apoptosis damages induced by high glucose.
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To improve the quality of teaching,stimulate students'interest in studying medical cytobiology,and nurture the medical students with ability of practice,solid theory and innovation,we have carried on discussions about different kinds of teaching methods and discovered such results:If teachers can integrate the most recent development of medical cytobiology with teaching,strengthen the relationship between the real life and the clinical practice,and update the teaching contents with multi-teaching methods,we will receive a satisfactory teaching effect.