ABSTRACT
In a survey of sarcocysts in muscle tissues obtained from 142 water buffaloes, 65% of the carcasses had sarcocysts. Macroscopic and two forms of microscopic sarcocysts, the spindle-shaped or fusiform sarcocysts commonly occurring in the muscles of the esophagus, throat and limbs, and the globular to oval-shaped sarcocysts which were the dominant form in the diaphragm and cervical muscle tissues were noted. Ultrastructural analysis of macroscopic and microscopic sarcocysts and their cyst wall revealed two distinct species of Sarcocystis: the macroscopic species, Sarcocystis fusiformis which has been previously reported in Philippine carabaos possessing highly dendritic cauliflower-like projections emanating from the primary cyst wall, with annulated microfilaments and numerous electron dense granules: and the Sarcocystis levinei (Dissanaike and Kan, 1978) Huong, Dubey and Uggla. 1997 exhibiting a cyst wall with undulating and hair-like villar protrusions with expanded or dome-shaped base, intermediate finger-like, and distal tapering segments which at some points join to form conical tufts. Our findings represent the first report of S. levinei in the country supported with ultrastructural analysis of the sarcocysts and cyst wall, and likewise refute earlier published reports that all microscopic sarcocysts in Philippine carabaos are developing forms of the macroscopic species, S. fusiformis. Histopathological changes such as displacement and necrosis of the surrounding host muscle tissue were observed with macroscopic sarcocysts and histologically processed tissue samples containing microscopic fusiform sarcocysts. Necrotic myofibrils and mitochondria were evident in ultrathin sections.
Subject(s)
Animals , Buffaloes , Philippines , Sarcocystis/isolation & purification , Sarcocystosis/pathologyABSTRACT
Biological assay of the crude petroleum ether extract of the annato showed that it contains 1300 I.U. of vitamin A activity per gram. As one gram of the oil extract is equivalent to about 50 grams of annato seeds it is obvious that annato is not a rich source of vitamin ATests for the presence of carotene in the crude oily extract by isolating this pigment by the phase separation method proved to be negativeThe water extract concentrate of the annato when assayed on rats for vitamin A activity by ther therapeutic and preventive methods of assay did not give any response, showing that coloring food preparations with this extract will not increase their vitamin A content. (Summary and Conclusions)
ABSTRACT
Se evaluó la aceptabilidad de tres antibióticos conocidos por su resistencia a beta-lactamasas en una muestra integrada por niños. Los resultados fueron comparados con los obtenidos en adultos. La aceptabilidad al sabor de los antibióticos en suspensión fue calificada con base en una escala analógica de 10 cm. Tanto en niños como en adultos, se asignó la calificación más alta a la azitromicina. Las significativas diferencias entre los sabores sugieren que este factor debe ser tomado en cuenta en todo estudio de aceptabilidad