ABSTRACT
O estudo farmacognóstico de Indigofera microcarpa Desv. (Fabaceae) foi realizado enfocando aspectos farmacobotânicos e fitoquímicos para fins diagnósticos. A anatomia das folhas, caule e raízes foi descrita e a presença de características típicas da família e do gênero Indigofera foram confirmadas. A triagem fitoquímica revelou a presença de fenóis, taninos, flavanonas, esteróides, triterpenóides, saponinas e bases quaternárias
Subject(s)
Pharmacognosy , Plant Extracts , Plants, Medicinal , Plant Leaves , Plant Stems , Plant RootsABSTRACT
Arrabidaea harleyi A.H. Gentry (Bignoniaceae) is an ornamental plant found in some regions of the Atlanticforest in Brazil. From its bark a mixture of verbascoside and isoverbascoside was isolated. This mixture was shown to be active against Staphylococcus aureus, Micrococcus luteus, Bacillus subtilis, Bacillus mycoides, Enterecoccus faecalis, Escherichia coli, Serratia marcensis and Candida albicans. The minimal inhibitory concentration (MIC) was established by diffusion method
Subject(s)
Pharmacognosy , Plants, Medicinal , Plant Extracts , Plant StemsABSTRACT
Streptoverticillium sp, bactéria pertencente a família Streptomycetaceae, foi isolada de amostras de solo do câmpus da Universidade Federal do Pernambuco, e destacou-se pela atividade antibacteriana e antifúngica de seus matabólitos secundários. O extrato etanóico bruto foi submetido ao fracionamento cromatográfigo. Das substâncias isoladas, duas apresentaram atividade antimicrobiana em relação a sete espécies de Candida. Em testes biológicos realizados em camundongos, determinou-se que a maior dose do extrato bruto na qual não houve óbitos foi de 150 mg/kg e todos os animais morreram com a administração de 600 mg/kg. Para o extrato etanóico de Streptoverticillium sp observou-se IC50 de 13 ug/mL e taxa de inibição tumoral igual a 61,5 por cento para o carcinoma de Ehrlich e 75 por cento em animais com sarcoma 180.
Subject(s)
Rats , Antifungal Agents , Antifungal Agents , Candida albicans , Cytotoxicity, Immunologic , Streptomycetaceae , Anti-Bacterial Agents/toxicityABSTRACT
The Barbatimão is a tree which bark is rich in tannin. It is used on popular medicine as a wound healing agent, in the treatment of gastric lesions, as anti-leishmanial agent and as anti-inflammatory. Red blood cells (RBC) are labeled with technetium-99m (Tc-99m) and are utilized in many procedures in nuclear medicine. Some authors have reported that drugs (natural and synthetic) can alter the labeling of RBC with Tc-99m. This study evaluates the effect of barbatimão infusion on the labeling of red blood cells (RBC) and plasma (P) proteins with Tc-99m. Heparinized blood from Wistar rats was incubated with NaCl 0.9 percent as control and different concentrations of barbatimão infusion. Following the addition of stannous chloride (SnCl2), as reducing agent, and Tc-99m, as sodium pertechnetate, the blood samples were centrifuged. P and RBC were separated and were also precipitated with trichloroacetic acid 5 percent. Insoluble (IF-P and IF-RBC) fractions were isolated. The percentage of radioactivity in all the samples was determined. All the barbatimão infusion concentrations decreased the labeling of RBC, IF-P and IF-RBC. We can speculate that the barbatimão infusion interfered on the labeling of RBC due to the redox properties and/or it can also act as a chelator of the stannous ion.