ABSTRACT
ABSTRACT Objectives: We aimed to investigate the prevalence of the BRAF (V600E) mutation in consecutive cases of papillary thyroid carcinoma (PTC) in patients diagnosed and treated at the Hospital Sao Rafael (Salvador, BA, Brazil) and evaluate its association with clinical and pathological characteristics of PTC. Subjects and methods: We retrospectively enrolled in the study a total of 43 consecutive PTC patients who underwent total thyroidectomy. We performed DNA extraction from formalin-fixed paraffin-embedded (FFPE) tumour tissue samples. Polymerase chain reaction (PCR) and direct sequencing were used to determine BRAF (V600E) mutation status. Univariate and multivariate logistic regression analyses were employed to identify independent associations. Results: The prevalence of BRAF (V600E) mutation was 65.1% (28/43). A high frequency of older patients (p value: 0.004) was observed among the BRAF-mutated PTC group and, in contrast, a low frequency of concurrent Hashimoto's thyroiditis (HT) (p value: 0.011) was noted. Multivariate analysis confirmed that older age (OR: 1.15; 95% CI: 1.00 - 1.33; p value: 0.047) and HT (OR: 0.05; 95% CI: 0.006-0.40; p value: 0.005) were independent factors associated with BRAF (V600E) mutation. Conclusion: We found a high prevalence of BRAF (V600E) mutation in PTC cases. Older age and no concurrent HT were independently associated with BRAF (V600E) mutation.
Subject(s)
Humans , Male , Female , Child , Adolescent , Adult , Middle Aged , Aged , Young Adult , Thyroid Neoplasms/genetics , Proto-Oncogene Proteins B-raf/genetics , Thyroid Cancer, Papillary/genetics , Mutation/genetics , Prognosis , Brazil/epidemiology , Thyroid Neoplasms/epidemiology , DNA Mutational Analysis , Prevalence , Cross-Sectional Studies , Retrospective Studies , Age Factors , Hashimoto Disease/complications , Hashimoto Disease/genetics , Thyroid Cancer, Papillary/complications , Thyroid Cancer, Papillary/epidemiologyABSTRACT
Num estudo caso-controle conduzido em Salvador, Bahia - Brasil, foi entrevistado um total de 404 indivíduos. Informaçöes sobre a situaçäo sócio-econômica, condiçöes de saúde oral, uso de anti-séptico bucal, consumo de fumo e álcool foram comparadas com controle de sexo, raça e idade. Os resultados indicam o consumo de fumo e álcool como fatores de risco significantes. A presença de dentes fraturados na cavidade oral e o uso de anti-séptico bucal e de prótese mal adaptada foram identificados como fatores de risco adicionais