ABSTRACT
Puba or carimã is a Brazilian staple food obtained by spontaneous submerged fermentation of cassava roots. A total of 116 lactobacilli and three cocci isolates from 20 commercial puba samples were recovered on de Man, Rogosa and Sharpe agar (MRS); they were characterized for their antagonistic activity against foodborne pathogens and identified taxonomically by classical and molecular methods. In all samples, lactic acid bacteria were recovered as the dominant microbiota (7.86 ± 0.41 log10 CFU/g). 16S-23S rRNA ARDRA pattern assigned 116 isolates to the Lactobacillus genus, represented by the species Lactobacillus fermentum (59 isolates), Lactobacillus delbrueckii (18 isolates), Lactobacillus casei (9 isolates), Lactobacillus reuteri (6 isolates), Lactobacillus brevis (3 isolates), Lactobacillus gasseri (2 isolates), Lactobacillus nagelii (1 isolate), and Lactobacillus plantarum group (18 isolates). recA gene-multiplex PCR analysis revealed that L. plantarum group isolates belonged to Lactobacillus plantarum (15 isolates) and Lactobacillus paraplantarum (3 isolates). Genomic diversity was investigated by molecular typing with rep (repetitive sequence)-based PCR using the primer ERIC2 (enterobacterial repetitive intergenic consensus). The Lactobacillus isolates exhibited genetic heterogeneity and species-specific fingerprint patterns. All the isolates showed antagonistic activity against the foodborne pathogenic bacteria tested. This antibacterial effect was attributed to acid production, except in the cases of three isolates that apparently produced bacteriocin-like inhibitory substances. This study provides the first insight into the genetic diversity of Lactobacillus spp. of puba.
Subject(s)
Humans , Fermentation , Genetic Variation , In Vitro Techniques , Lactobacillus plantarum/genetics , Lactobacillus plantarum/isolation & purification , Manihot/genetics , Polymerase Chain Reaction/methods , Food Samples , MethodsABSTRACT
Os relatos da esquistossomose mansônica acometendo o sistema nervoso central (SNC) são pouco frequentes, sendo raramente descrito no encéfalo, onde os ovos do Schistosoma mansoni usualmente provocam reação granulomatosa comportando-se como lesão expansiva. Um paciente do sexo masculino, procedente de São Vicente de Ferrer, Baixada Ocidental Maranhense, área endêmica da doença, 27 anos de idade, apresentou tumor no cerebelo, o qual foi tratado cirurgicamente. O estudo histopatológico da lesão cerebelar, demonstrou extensa reação granulomatosa ao redor de ovos em desintegração de Schistosoma mansoni. Os granulomas exibiam diferentes estágios de evolução, desde necrótico exsudativo até produtivo. Este caso representa o primeiro de neuroesquistossomose relatado no estado do Maranhão. A evolução pós-operatória do paciente caracterizou-se por déficit e incoordenação motora dos membros superior e inferior direito associado a tremores.
The involvement of the central nervous system in the Schistosomiasis mansoni is uncommon and in the literature exist few reports in relation this occurrence in the brain, in that the schistosoma eggs, usually promote a granulomatous reaction that remember an expansive tumour. A patient, male sex, from São Vicente de Ferrer (MA), 27 years old, had a tumour in the cerebellum, that had surgical therapy. Later histological studies of the cerebellar material of the patient, demonstrated an extensive granulomatous reaction around schistosoma eggs. The granulomatous reaction present different stages of evolution, since degenerative necrosis to organization granulomatous reaction. This is the first report from Maranhão (Brazil). The evolution of the patient was characterized for incoordination of members and trembling.
Subject(s)
Adult , Animals , Humans , Male , Brain Diseases/parasitology , Schistosomiasis/complications , Schistosoma mansoni , Brain Diseases/pathology , Schistosomiasis/pathologySubject(s)
Child , Adolescent , Adult , Middle Aged , Humans , Male , Female , Pulmonary Heart Disease , SchistosomiasisABSTRACT
Defeito do septo interventricular por traumatismo toracico fechado foi diagnosticado em um paciente de 11 anos de idade, do sexo masculino, apos acidente automobilistico. A correlacao cirurgica foi realizada cinco meses apos o trauma. Nao houve intercorrencias no pos-operatorio e o paciente, apos um ano, encontra-se assintomatico. Alem do relato deste caso, os autores fazem uma revisao da literatura, abordando a incidencia, mecanismo da rotura septal, suas caracteristicas clinicas, indicacoes cirurgicas e resultados. Chamam a atencao para a importancia do reconhecimento precoce do defeito septal traumatico e avaliacao do grau de lesao e da presenca de lesoes associadas, tendo em vista a evolucao e possivel indicacao cirurgica, enfantizando que a ausencia de fraturas toracicas nao exclue severa lesao cardiaca