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Purpose@#Inflammatory cytokines are thought to be involved in the pathogenesis of intracranial aneurysm (IA), although resultsamong studies in the literature are inconsistent. This article sought to review studies on the associations among polymorphismsin inflammatory cytokine genes and IA risk and to provide recommendations for future research. @*Materials and Methods@#A systematic search of PubMed, Embase, and Web of Science was conducted up to August 4, 2019. Theassociations between polymorphisms of inflammatory cytokine genes and IA risk were estimated by pooled odds ratios (ORs) and95% confidence intervals (CIs). Subgroup analyses were performed according to race. Qualitative systematic review was conductedfor variants that were studied in only one study. All analyses were performed using STATA 12.0. @*Results@#13 studies investigating the associations between polymorphisms in five inflammatory cytokine genes (TNF-α, IL-1α, IL-1β, IL6, and IL-12B) and IA were reviewed. Combined results showed that the A allele of TNF-α rs1800629 polymorphism has aprotective effect against IA (dominant model: OR=0.65, 95% CI=0.47–0.89, p=0.007). No associations were identified between polymorphismsin IL-1α rs1800587, IL-1β rs16944, IL6 rs1800795 and rs1800796, or IL-12B rs3212227 and IA risk. @*Conclusion@#This review demonstrated an association between TNF-α rs1800629 polymorphism and IA in Caucasians, illustratingthe potentially important role of genes involved in inflammation in IA.
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A method for quantitative detection of florfenicol by colloidal gold lateral flow immunoassay was developed.The experimental conditions including pH value, concentrations of antibody in the process of conjugation between the colloidal gold and antibody, amount of gold-labeled antibody, concentration of the antigen sprayed on test lines (T line), and detection time were optimized.With a colloidal gold strip reader, the signal intensity of T lines and control lines (C line) on lateral flow strips was recorded.The T/C ratio of negative control and positive samples was defined as B0 and Bx, and the standard curve was established by plotting the Bx/B0 ratio against the concentration of florfenicol.This assay showed a good linear range from 0.1 to 1.5 ng/mL with the limit of detection of 0.08 ng/mL, while the result could be obtained within 15 min.The result showed that this quantitative method was convenient and rapid, and could be used in screening a large amount of samples on site.
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Objective To observe the infecion status and drug resistant rate of mycoplasma in infertility patients in Puyang city and to guide useing drug rationally.Methods A total of 500 women in reproductive period in Puyang city were recruited in this study,collected their vaginal discharge specimen,then cultured and detected ureaplasma urealyticum(Uu) and mycoplasma hominis(Mh) by liquid culture method and solid culture method.The women with positive results were divided as the pregnancy group and infertility group.The sensitivity rate of mycoplasma to 12 kinds of antibiotic were tested.Results The positive rate of mycoplasma in all the 500 women was 44.0%(220/500),the constituent ratio of pregnancy group(60 cases) was 27.3%,and the constituent ratio of infertility group (160 cases) was 72.7%.The difference was statistically significant between infertility group and pregnancy group(P<0.05).The Uu infection rates in pregnancy group and infertility group were 71.6% and 76.2% respectively,and the Mh infection rates were 11.6%,11.2% respectively.The rates of infecting Uu and Mh at the same time were 16.6% and 12.5%.The mycoplasma in and infertility group the pregnancy group were sensitive to spectinomycin,doxycycline and capminocim,which were 91.8%,92.1%,88.3%;90.9%,90.9%,87.3%.Conclusion The infection rate of Uu is higher than Mh in patients with infertility,which is resistant to quinolone classes and large ring lactone class antibiotic.
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Objective To design and synthesze novel pyrrolopyridine as integrase inhibitors. Method The launched anti-HIV drug raltegravir was selected as template compounds. According to the concept of bioisosterism and molecular docking ,a series of pyrrolopyridine compounds(6a-6t)were designed,synthesized and their anti-integrase 3′-processing activity were analyzed. Re?sults The designed compounds were successfully synthesized and the structures of these compounds were confirmed by 1H NMR,13C NMR and ESI-HRMS. The anti-IN 3′-P activity of these compounds were also measured. The binding mode and docking energy of rep?resentative compounds were analyzed. Conclusion The structure-activity relationships of these compounds were also analyzed by the results of docking. These results lay the foundation for the further optimization of these compounds.
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Objective To study a method of microwave-assistant PEGylation of polystyrene resin. Methods Modify the commercial Merrifield resin with PEG-200, and study the influence of the yield under different conditions. The self-prepared PEG resin was further derived to PEG-wang resin; kinds of amino acids were anchored, and the loading rates were compared with commercial wang resin. Result A general series react conditions were preferred, which was performed with 5 g resin, 50 ml PEG-200, irradiating for 15 min ×2 at 600 W, preset temperature was 170 ℃. The resin was then transformed to PEGylated Wang resin and anchored with amino acids, the yields were satisfied. Conclusion The multi-mode microwave assistant PEGylation of Merrifield resin method was first reported in this paper. This method has the advantages of simple operation,fast preparation, mild reaction conditions and good yield, which was much better than the traditional method.
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Objective Preparation of the Phakellistatin 13 by using of solid-phase peptide synthesis method and photolabile protecting group. Methods By using of the DHP resin as the solid-phase supporter, a threonine derivative which was protected by the self-prepared photolabile protecting group was anchored to. The linear peptide was assembled via standard procedure with the threonine as the starting amino acid. After that, the photolabile protecting group was removed by UV irradiation, and the cyclization was completed while the peptide was still attached on the beads. The free cyclic peptide was then cut off by acid. Results The photolabile 2-(2-nitrophenyl)-propanol (Npp-OH) was synthesized in one step from o-nitroethylbenzene. With the Npp group, the carboxyl group of threonine was blocked and Phakellistatin 13 was synthesized. The Phakellistatin 13 was identified by high resolution mass spectrometry, indicated that the strategy is quite available, which offer an efficient method for cyclic peptide synthesis. Conclusion The Npp group had the advantages of easy prepared, high yields for protection and deprotection, be stable to the acidic and alkaline conditions, therefore it is an excellent third division protecting group. With this group, the cyclic peptide can be synthesized efficiently.
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Objective:To describe the status of subjective well-being among junior high school students in a city of Jiangxi Province and to explore the relationship between family functioning and subjective well-being.To explore the relationship between growth environment and subjective well-being among ju-nior high school students in the perspective of family functioning and to provide basis and data for the mental health education among junior high school students.Methods:630 students in a junior high school in Jiangxi Province were sampled by stratified cluster sampling,and 103 junior high school students from 18 villages were sampled by household survey.They were investigated by subjective well-being scale for adolescents and Family Assessment Device.Results:(1)The overall subjective well-being of junior high school students was in the lower-middle level.Regarding all the dimensions of subjective well-being,they had the highest satisfaction on family and friendship,lowest academic satisfaction,low positive as well as passive emotion.(2)Girls had higher satisfaction on friendship and freedom than that of boys;children from families with more than one kids had more negative emotion than that of single child;boarding students had higher satisfaction on family,academics and freedom than that of non-boarding students;family residence and structure affected the satisfaction on family;grade and learning achievement affected some dimensions of subjective well-being among junior high school students.(3)Pearson correlation analysis and multiple linear regression analysis showed that some dimensions of family functioning were related to certain dimensions of subjective well-being among junior school students,and family functioning could predict the level of subjective well-being.Conclusion:The overall subjective well-being of junior high school students is in the lower-middle level,and further improving is needed.Family functioning has a great influence on junior school students' subjective well-being.Junior high school students who have healthy family functioning are more likely to have higher life satisfaction,and tend to experience higher subjective well-being.