ABSTRACT
Objective To investigate the effect of Mongolian medicine naru-3 on the apoptosis of mouse hepatic cells induced by radiation. Methods Kunming mice were radiated with 60Coγ-irradiation, the 30-day survival rate of mice and the average life span of dead mice post-irradiation were observed. The apoptosis of hepatic cells from irradiatied mice was detected by FACS and TUNEA expression level of p53 was examined. Results Administration of the medicine resulted in increase of 30-day survival rate and prolongation of average life span of the dead mice. The apoptosis rate of spleen cells from mice treated with Mongolian medicine naru-3 decreased significantly and the ex pression level of p53 was also inhibited significantly. Conclusion The results indicated that Mongolian medicine naru-3 had radio protective function and its mechanism might be related to the suppression of apoptosis of radiationsensitive cells.
ABSTRACT
BACKGROUND: Researches indicate that interleukin-6 (IL-6) in blood has changed during the process of the edema and infection of spinal cord.It is found that IL-6 participates in the course of spinal cord lesion according to the further researches. It is also presumed that the IL-6 is correlated with the edema of spinal cord accompanied nervous system diseases.OBJECTIVE: To observe the effect of the receptor antagonist of the IL-6 on the traumatic edema of spinal cord, and explore the effect of IL-6 in the process of the edema of spinal cord.DESIGN: Randomized control experiment with animals as subjects.SETTING: Department of Orthopedic, Hospital Affiliated to Guangdong Medical College.MATERIALS: The experiment was completed from January to December 2004 in the Animal Experimental Center of Guangdong Medical College.Forty male Wistar rats were selected and randomly divided into the normal group, the wound group, the saline control group and the receptor antagonist of the IL-6 treated group with 10 rats in each group.METHODS: ① The receptor antagonist of the IL-6 treated group: Blast injury of the thoracic spinal cord was made by the sap pressure, and the injection with the receptor antagonist of the IL-6 was given at the injured region with microinjector. ② The wound group: There was not the procedure of administration and placement of the tube. ③ The saline control group: The IL-6 was replaced with the sterile-physiological saline solution (SPSS), and the other procedures were the same as those of the receptor antagonist of the IL-6 treated group. ④ The normal group: There was not any treatment. All rats in each group were detected with magnetic resonance. The water content in the spinal cord was determined with the dry and humid weight method, and the status of the edema of spinal cord was observed with the electron microscope and the pathological image analysis after dyeing with hematine-eosine.MAIN OUTCOME MEASURES: ① The results of the examination of the rats in each group with the magnetic resonance. ② The water content in the spinal cord of rats in each group. ③ The status of the edema of spinal cord was observed with the electron microscope and the pathological image analysis.RESULTS: All the 40 rats entered the result analysis. ① The edema of the T2 w eighting image in the receptor antagonist of the IL-6 treated group was relieved as compared with that in the wound group and the saline control group. ② The water content of the injured region in the receptor antagonist of the IL-6 treated group, the wound group and the saline control group was higher than that in the normal group [ (81.68±1.39)%, (79.72±1.49)%, (82.59±1.12)%, (77.19±0.64)% ,P ＜ 0.01].The water content of the injured region in the wound group was higher than that in the normal group(P ＜ 0.01), and the receptor antagonist of the IL-6 treated group was lower than that of the wound group (P ＜ 0.01). ③The cell body of the gray substance was contracted, the inter-space around the cell was enlarged and the nerve cells were lost in the wound group;The saline control group was not significantly different as compared with the wound group; The cellular edema, degeneration and the necrosis in the receptor antagonist of the IL-6 treated group was milder than that in the wound group.CONCLUSION: The receptor antagonist of the IL-6 has the therapeutical effect on the traumatic edema of spinal cord. The endogenous IL-6 participates in the development of the traumatic edema of spinal cord.
ABSTRACT
[Objective]To study NGF,BDNF gene modified bone marrowe stem cells transplantation with vein on spinal cord injury.[Method]Spinal cord injured rats were divided in to 3 groups.Group C consisted of the rats treated with the transplantation of modified NGF,BDNF genes BMSCs;group B of the rats with transplantation of BMSCs without genetic modification;group A of the rats without treatment and group D was the normal contral.At 2,6,10 weeks the rats were examined with GBS score and neurological function were tested.[Result]The vein with tintracord transplantation of modified BMSCs group was improved by the GBS score and neurological outcome of injured spinal cord was achieved well.[Conclusion]The vein with transplantation of modified NGF,BDNF genes BMSCs can recover of spinal cord injury.