Expression of NKG2D and NKG2A with their ligands MHC-I A/B and HLA-E in acute leukemia patients and its significance / 中国实验血液学杂志

This study was aimed to explore the difference of NK cell receptor NKG2D and NKG2A expression on NK cells and CD3(+) T cells and their ligand MHC-I A/B (major histocompatibility complex class I-related chains A/B) and HLA-E expression in leukemia cells, as well as its immunological significance. Flow cytometry was used to detect the killing rate of NK92 cells to 8 leukemia cell lines, and the expression of NKG2D and NKG2A on NK cells and CD3(+) T cells as well as their ligand MHC-I A/B and HLA-E expression on leukemia cells. The results indicated that the NK92 showed different killing activity to different leukemia cell lines. The positive expression rate of NKG2D and NKG2A on NK cells and CD3(+) T cells in ALL patients was no significantly different from that in AML patients (p > 0.05), but positive expression rate of MHC-I A/B and HLA-E in ALL patients was obviously higher than that in AML patients (p < 0.05). It is concluded that there is difference of immune cell function between ALL and AML patients, this difference may be associated with the expression difference of NKG2D and NKG2A ligands on leukemia cells while does not associated with the killing and inhibiting receptors expressed on NK cells and CD3(+) T cells.

Meta-analysis of the risk factors for coronary artery lesion secondary to Kawasaki disease in Chinese children / 中华儿科杂志

<p><b>OBJECTIVE</b>Coronary artery lesion (CAL) is a serious complication of Kawasaki disease (KD). Whether there is CAL and the severity are the most critical factors of the prognosis of KD. The incidence of KD is currently increasing year by year. KD has replaced rheumatic fever as the main entity of acquired heart disease of children. This study aimed to identify risk factors of CAL secondary to KD and take early interventions to prevent CAL or reduce its incidence.</p><p><b>METHOD</b>Literature search was performed at Chinese Academic Literature Main Database, Chinese Science and Technology Periodical Database, Wanfang Periodicals and Dissertation Database, and the Chinese Biomedical Literature Database comprehensively, besides, retrospective retrieval and manual retrieval were also performed from the domestic public actions and the dissertations dating from January, 2000 to December, 2009. RavMan 4.2 provided by Cochrane was used for meta analysis. Fixed or random model was selected according to the results of heterogeneity test. Sensitivity analysis was done according to the different results. The publication bias was evaluated by funnel plots. Odds ratio (OR) and 95% confidence interval (CI) were estimated in the dissertation.</p><p><b>RESULT</b>Twenty studies were confirmed to be eligible. All the 20 studies were retrospective. OR and 95%CI of the risk factors were as follows: age ≤ 1 year, OR = 1.58, and 95%CI (1.23, 2.04), P = 0.0004; male gender, OR = 1.48, 95%CI (1.29, 1.71), P < 0.000 01; WBC > 20 × 10(9)/L, OR = 1.73, 95%CI (1.32, 2.26), P < 0.0001; C-reactive protein (CRP) > 100 mg/L, OR = 2.37, 95%CI (1.49, 3.77), P = 0.0003; fever duration > 10 d, OR = 3.23, 95%CI (2.08, 5.02), P < 0.000 01; use of intravenous gamma globulin (IVIG) > 10 d, OR = 2.50, 95%CI (1.98, 3.16), P < 0.000 01.</p><p><b>CONCLUSION</b>The high risk factors for coronary artery lesion secondary to Kawasaki disease are age ≤ 1 year, male, WBC > 20 × 10(9)/L, CRP > 100 mg/L, fever duration>10 d, and use of intravenous gamma globulin (IVIG) > 10 d.</p>

Conditioned mediums of different rat cerebral microvascular endothelial cells against damage of ischemia and ischemia/reperfusion neurons / 中西医结合学报

OBJECTIVE: Using the method of lactate dehydrogenase (LDH) assay, to observe the activities of rat cerebral microvascular endothelial cells (CMECs) intervened by Tongluo Jiunao Injection (TLJNI), a traditional Chinese compound drug removing toxin to dredge brain collaterals, and then further study the effects of different kinds of conditioned mediums (CMECs-CM) of cerebral microvascular endothelial cells on ischemia and ischemia/reperfusion cerebral cortex cells, and to probe into the drug pharmacological mechanisms of CMECs in modulating the neurons. METHODS: Three kinds of CMECs (normal, ischemic and ischemic/reperfusional) were all treated by TLJNI previously, and then the three pairs of CMECs-CM without serum were collected respectively for LDH assay. Rat cerebral cortex neurons were also primarily cultured and then divided into similar three groups (normal, ischemic and ischemic/reperfusional). The neuron responses caused by CMECs-CM at different concentrations were observed by using LDH transudation rate assay. RESULTS: The LDH release values of ischemic and ischemic/reperfusional CMECs with TLJNI treatment were obviously reduced (P<0.01) compared with the same kinds of CMECs untreated. For ischemic neurons, both conditioned medium of ischemic CMECs (Is-CM) and conditioned medium of ischemic CMECs with drug treatment (IsT-CM) in high concentration of 100% increased the LDH transudation rate (P<0.01), while in low concentration of 10%, IsT-CM reduced the transudation rate (P<0.05). For ischemia/reperfusion neurons, all kinds of CMECs-CM reduced the transudation rate respectively (P<0.05 or P<0.01). As far as each group concentration was concerned, 10% or 50% showed relatively stronger effects, and both conditioned medium of normal CMECs (N-CM) group and conditioned medium of ischemic/reperfusional CMECs (Rp-CM) group had statistical significance (P<0.05 or P<0.01). For normal neurons, all kinds of CMECs-CM increased the transudation rate respectively (P<0.05 or P<0.01). As far as each group concentration was concerned, only conditioned medium of normal CMECs (N-CM) had statistical significance (P<0.05 or P<0.01). CONCLUSION: The study shows that TLJNI is capable of preventing the damage of CMECs from both ischemia and ischemia/reperfusion states. Chinese drug can restrain the brain ischemia and ischemia/reperfusion damage by the media that CMECs modulate the neurons, demonstrating the pharmacological mechanisms of TLJNI. This work also indicates that there exist some active substances against ischemia/reperfusion injury secreted from CMECs-CM with TLJNI treatment.

The comparison of efficacy of different dosage regimen of recombinant human tumor necrosis factor receptor-Fc fusion protein in Chinese ankylosing spondylitis patients / 中华风湿病学杂志

0.05).In addition,in different medication intervals and the same total dosage(200 mg),there was no difference in the number of patients who reached ASAS20,ASAS50 anti BASDAI50 in both groups.The changes of other parameters were not observed.Conclusion Two dosages and different medication interval of rhTNFR-Fc have similiar efficacy onset time and maintenee period.Mean- while,at the same total dosage,there is no signifieant difference in therapeutic effect in the two dosage groups. However,50 mg(1/7 d)regimen has better compliance than 25 mg(1/3 d).