ABSTRACT
Objective To summarize the experience of improved nonmyeloablative hematopoietic stem cell transplantation(NSCT) in the treatment of severe aplastie anemia. Methods Seventeen patients with Severe Aplastic Anemia received NSCT after a nonmyeloablative conditioning.The patients were conditioned with decreased dosage of immunosuppressive agents of CTX and anti-lymphoid cell globulin or anti-thymus gland cell globulins;CsA and MMF were used to prevent the graft-versus-host disease (GVHD) after transplantation. Results 75 % of severe aplastic anemia in patients with type Ⅰ and 60 % of severe aplastic anemia in patients with type Ⅱ achieved rapid hematopoietic reconstitution, with good prognosis and implantation rate with fewer complieations and light symptoms were observed. Conclusion NSCT is an effective treatment of severe aplastic anemia.
ABSTRACT
Objective To study the therapeutic effect of TK/GCV in the treatment of acute graft-versus-host disease in H-2 haploidentical mice induced by CD34 + cells mixed with TK + T lymphocytes transplantation.Methods CD34 + cells were separated from male parental donors,bone marrows,meanwhile T lymphocytes were gained from their spleens and transduced by retroviral vectors comprising TK gene. Both were mixed and transfused into female F1 recipient mice,CD34 + cells and TK + T lymphocytes each F1 recipient mouse received were 1?10 5 and 1?10 7,respectively. GCV was administered at the dosage of 50 mg ?kg -1 ?day -1 for 7 by intraperitoneal injections on the day 0 or 7 after transplantation.Results Acute GVHDs appeared in all mice. Survival time of the mice treated with GCV on the day 0 or 7 after transplantation was ( 26.6 ? 4.8 ) days and ( 40.5 ? 8.4 ) days,respectively. Therapeutic effect of GCV on the day 7 posttransplantation was better than that on the day 0 posttransplantation ( P