ABSTRACT
Objective:To observe the efficacy of acitretin combined with traditional Chinese medicine in the treatment of pustular psoriasis.Methods:From July 2011 to October 2017, 91 patients with pustular psoriasis admitted to the Second People's Hospital of Changzhi were randomly divided into treatment group( n=31), control group Ⅰ ( n=30) and control group Ⅱ(n=30) according to the digital table.The patients in the three groups were treated with acitretin combined with traditional Chinese medicine, oral acitretin and traditional Chinese medicine, respectively.The therapeutic effects, the time required for hypothermia, the time required for the complete disappearance of pustules and the average length of hospitalization were compared among the three groups. Results:The total effective rate was 93.55%(29/31) in the treatment group, 70.00%(21/30) in the control group Ⅰ and 73.33%(22/30) in the control group Ⅱ.The total effective rate in the treatment group was significantly higher than that in the two control groups(χ 2=5.720, 4.546, all P<0.05). There was no statistically significant difference in total effective rate between the control group Ⅰ and the control group Ⅱ( P>0.05). The fever allaying time, completely disappearance time of pustules, average hospital stay in the treatment group were (4.24±0.86)d, (7.38±0.72)d, (23.46±0.61)d, respectively, which in the control group Ⅰ were (7.45±0.92)d, (10.45±0.52)d, (30.01±0.82)d, respectively, which in the control group Ⅱ were (8.56±0.78)d, (11.56±0.35)d, (31.53±0.68)d, respectively, which in the control group Ⅰ were significantly shorter than those in the control group Ⅱ( t=14.083, 19.037, 35.476, all P<0.05), which in the treatment group were significantly lower than those in the control group Ⅰ and the control group Ⅱ( t=20.529, 28.682, 48.826, 5.041, 9.699, 7.816, all P<0.05). Conclusion:Acitretin capsule combined with traditional Chinese medicine is effective in the treatment of pustular psoriasis, which can control body temperature in a short time, shorten the time of disappearance of pustule, relieve the pain of patients, and shorten the time of hospitalization at the same time.
ABSTRACT
Objective To get a new approach for conserving the germplasm of Siraitia grosvenorii Methods Shoots, 0.8—1 cm in length, excised from test-tube plantlets which were subcultured 15 d, were precultured. Then its shoot-tips about 2—3 mm in length were dissected and loaded with 60% PVS2 at 25 ℃, and dehydrated with 100% PVS2 at 0 ℃, changed for fresh 100% PVS2 prior to directly plunging into liquid nitrogen. After cryopreservation for 24 h, the shoot tips were thawed, rinsed in MS+1.2 mol/L sucrose medium, blotted with filter papers, then plated on the MS + 1.0 mg/L 6-BA+0.05 mg/L NAA + 0.1 mg/LGA3 + 0.8% agar + 45 g/L sucrose medium at 25 ℃ for 7 d in dark prior to exposure to the light. The root medium for regeneration plantlets was 1/2 MS + 0.2 mg/L NAA + 30 g/L sucrose. Results Shoots were precultured for 3 d on MS + 0.7 mol/L sucrose medium, then its shoot-tips loaded for 40 min, dehydrated for 50 min. After cryopreservation, the shoot tips were rapidly thawed in water at 40 ℃, then rinsed for 40 min. The survival rate of shoot-tips plated on the recovering medium in one week was 100%, and regeneration rate after 30 d was 78.33%, which was the highest. The regeneration plantlets inoculated on root medium were reconstructed integrating plantlets. Conclusion The method of vitrification to cryopreserve the germplasm of S. grosvenorii is a simple way with high survival rate and normal regeneration plants.