ABSTRACT
Objective To evaluate the clinical significance of antinuclear antibody (ANA) and ANA repertoire in the diagnosis of systemic sclerosis (SSc). Methods ANAs were tested in 64 pa tients with SSc and 30 healthy controls by applying indirect immunofluorescence (IIF) and immunoblotting (IB). Results IIF-ANA assay showed that the positive rate of ANAs was 98. 4% (63/64) in SSc, and ANA staining was mainly shown as homogeneous-nucleolar pattern; IB-ENA test indicated that the antinuclear antibodies were mainly anti-Scl-70, anti-SS-A and anti-nRNP in SSc, and the positive rate was respectively 65.1% (41/64), 29.7% (19/64) and 6.3% (4/64). Conclusion Existence of multiple antibodies in SSc patients may hint that the patients might have other autoimmune diseases or increased risk for other autoimmune diseases. Besides, IB assay significantly increases the sensitivity and specificity of ANAs measurement, and contributes to the diagnosis of SSc.
ABSTRACT
Objective To study the clinical significance of detection of infectivity index in patients before transfusion. Methods A total of 12 863 patients in hospital were examined for hepatitis B virus surface antigen(HBsAg),anti-hepatitis C virus(anti-HCV),anti-AIDS virus(anti-HIV)and treponemiasis test(TRUST)before operation and transfusion. Results The positive rates of HBsAg,anti-HCV,anti-HIV and TRUST were 12.59%,1.90%,0.02%and 0.80%respectively.Conclusion The detection of infectivity index before transfusion has a very,important role in the prevention of blood transmissible disease and reduction or medical treatment dispute caused by infection after transfusion.
ABSTRACT
Objective To evaluate ANA and ANAs as a indacation of systemic sclerosis diagnostic.Method ANAs were tested in 58 patients with SSc and 20 healthy controls by indirect immunofluorescence (ⅡF)and immunoblot(IB).Results ⅡF-ANA results indicated that the positive of ANAs were 98.3%(57/58)in SSc,and ANA pattern were mainly homogeneous-nucleolar,IB-ENA results indicated that the antinuclear antibodies were mainly anti-Scl-70.anti-SSA and anti-nRNP in SSc.The positive of them were 63.8%、31.O%and 5.2%.Conclusions It suggested that the patients might have other autoimmune diseases or the dangerous of other autoimmune diseases,and the IB kits of euroimmun got better positives and specificities to ANAs,the method could provide more helps to diagnosis of SSc.