ABSTRACT
Objective:To investigate the effects of troglitazone on cell proliferation and expression of intercellular adhesion molecule-1(ICAM-1) induced by tumor necrosis factor-?(TNF-?) in human mesangial cells.Methods:Human mesangial cells were cultured in RPMI-1640 media containing TNF-? with or without troglitazone.Cell proliferation was assessed by MTT;the mRNA and protein expression of ICAM-1 were measured by RT-PCR and ELISA;the protein expression of NF-?B was measured by immunohistochemistry.Results:Troglitazone inhibited the proliferation of mesangial cell.The mRNA and protein expression of ICAM-1 of the cell induced by TNF-? increased significantly(P
ABSTRACT
Objective To summarize clinical characteristics of primary nephrotic syndrome (PNS) in children. Methods The clinical and laboratory data of 964 children with PNS (including 747 new cases) were retrospectively analyzed,who were admitted between 1 August,1996 to 31 July,2006. Results Of 747 cases,568 were males and 179 females,with a median age of 4.7 years (0.3-17.3). The total number of children with PNS aged from 1 to 5 years was 385 (51.5%). Compared with children with nephritic type nephrotic syndrome (NNS),children with simple type nephrotic syndrome (SNS) had lower albumin (P0.05); Compared with normal values,children with PNS had lower IgG (P0.05). Main motivation of PNS relapse in children was upper respiratory infection,followed by irregular administration of glucocorticoids. Conclusion Children aged from 1 to 5 years are susceptible to PNS. There is humoral immunological dysfunction in PNS children. In the treatment of PNS,to relieve hyperlipidemia and to prevent and control infections is the key point. To strengthen propaganda and guidance about PNS is important as well.