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Objective To evaluate the effect of anti-Mueller hormone on ovarian reserve in female patients with systemic lupus erythematosus(SLE).Methods The clinical data of 80 women with SLE in the Department of Rheumatism of Zaozhuang Municipal Hospital from June 2015 to June 2018 were collected.The patients used cyclophosphamide before entering the group were selected as A group (35 cases),and patients who didn't use cyclophosphamide were selected as B group(45 cases),and 30 healthy female volunteers were selected as the control group at the same time.All of them were normal menstrual cycle.At the third day of the menstrual cycle,the levels of anti-Mueller hormones were detected by enzyme linked immunosorbent assay.The serum levels of estrogen and alveolar hormone were detected by microparticle enzyme immunoassay,and the number of ovarian sinus follicles in each group was recorded by transvaginal ultrasound.Results The incidence rate of thrombocytopenia,nephritis,hemolytic anemia and neuropsychiatric manifestations in A group were 54.29％ (19/35),85.71％ (30/35),37.14％ (13/35) and 22.86％ (8/35),respectively,which were significantly higher than those in B group [48.89％ (22/45),53.33％ (24/45),13.33％ (6/45),0.00％ (0/45)] (x2 =6.33,9.41,6.16,9.03,all P ＜0.05).The number of anti-Mueller hormone [(1.09 ± 0.33) g/L] and numbers of ovarian sinus follicle [(8 ± 2)] in A group were significantly lower than those in B group [(1.98 ± 0.62) g/L,(12 ± 4)] and control group [(3.37 ± 1.11) g/L,(15 ± 5)],which showed significant differences (F =46.07,25.90,all P ＜ 0.05),but there were no statistically significant differences in serum estrogen and follicular stimulating hormone levels among the three groups(F =1.16,0.97,all P ＞ 0.05).The level of anti-Mueller hormone in patients with SLE aged ＞ 30 years [(0.98 ± 0.31) g/L]was significantly lower than patients ≤30 years old[(1.86 ±0.60) g/L],and the level of anti-Mueller hormone in patients with cyclophosphamide accumulation ＞ 10 g[(0.36 ± 0.11) g/L] was significantly lower than that of 10 g [(1.76 ± 0.52) g/L] (t =7.54,11.30,both P ＜ 0.01).The results of Pearson correlation analysis showed that the anti-Mueller hormone showed negative correlation with the age of SLE and the accumulation of cyclophosphamide (r =-0.56,-0.45,both P ＜ 0.01).Conclusion The use of anti-Mueller hormone has good evaluation value for ovarian reserve function in female patients with SLE,and the level of anti-Mueller hormone is closely related to the age and the accumulation of cyclophosphamide.Therefore,cyclophosphamide should be used carefully in patients who are over 30 years old and the cumulative amount of cyclophosphamide over 10 g.
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Objective To investigate the anatomical changes and dose distribution deviations of locally advanced nasopharyngeal carcinoma ( NPC ) patients during intensity-modulated radiation therapy ( IMRT) . Methods Weekly cone beam CT ( CBCT) was applied to investigate the anatomical changes of enrolled 18 patients with locally advanced NPC. Dosimetric deviations were investigated with new IMRT plans after 20 fractions. Results The volumes of gross tumor volume ( GTVnx ) and GTVnd were decreased by 3. 15% and 5. 67% weekly, and decreased by a total of 22. 03% and 39. 68%, respectiely. The volumes of left and right parotids were decreased by 4. 93% and 5. 26% weekly, and decreased by a total of 29. 60% and 31. 56%, respectively. The volumes of GTVnd and parotids showed significant retraction in the first four weeks of radiation, then the retraction slowed down. The D95 of PGTVnd in Plan 2 decreased by 2. 20% (t=2. 382, P <0. 05) compared that in the initial Plan 1, while there was no significant difference on the D95 of PGTVnx , PTV1 and PTV2 . The Dmean , D50 and V30 of the left and right parotids in Plan 2 increased by 7. 34%, 12. 68%, 10. 90% (t = -3. 376, -3. 738, -3. 679,P<0. 05), and 6. 13%, 11. 17%, 9. 72% (t= -2. 550, -2. 446, -2. 673, P<0. 05), respectively, compared with Plan 1. The Dmean of larynx increased by 8. 69% in Plan 2 compared with Plan 1 ( t = -3. 099, P <0. 05). The D95 of PGTVnd in Plan(1+2) increased by 1. 37% (t= -3. 555, P<0. 05) compared with Plan 2. The Dmean, D50 and V30 of the left and right parotids in Plan(1+2) were decreased by 2. 90%, 2. 73%, 4. 62% (t=3. 089, 2. 718,2. 705, P < 0. 05), and decreased by 3. 49%, 3. 44%, 3. 80%(t=2. 781, 2. 958,4. 275, P<0. 05), respectively, compared with Plan 1. The Dmean of larynx decreased by 3. 29% (t=2. 747, P<0. 05) in Plan(1+2) compared with Plan 1. Conclusions The volumes of GTV and parotids of locally advanced NPC patients showed significant shrinks during IMRT. Replanning is necessary during the middle of IMRT to improve the target coverage and to spare the organ at risks ( OARs) .
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Objective To investigate the pollution condition of Salmonella in food with phage. Methods Salmonella in 413 samples of food were detected by the diagnosis of Salmonella phage with biochemical and serological identification. Results 119 Salmonella were detected in 99 positive samples and the isolating rate was 24%. Conclusion The disservice of Salmonella is mainly from the meat food and eggs. The detection method of phage is fast,convenient and reliable.
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Objective To study the relationship between levels of hyaluronic acid(HA),type Ⅳ collagen(ⅣC),Laminin (LN) in sera and hepatic fibrosis in patients with chronic hepatitis B.Methods 252 sera from patients with chronic hepatitis B were collected to measure hyaluronate,type Ⅳ collagen,laminin by radioimmunoassay,and sera HBV-DNA were also tested by quantitative fluorescence PCR respectively.Results Serum concentrations of HA,ⅣC and LN were remarkably increased with the progression of the disease,and reached the highest levels in chronic severe hepatitis and liver cirrhosis(P