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OBJECTIVE:To investigate therapeutic efficacy and safety of leosimendan in the treatment of acute left heart failure (ALHF) and its effects on left ventricular function indexes and serum markers.METHODS:A total of 110 patients with acute left ventricular function selected as research objects from No.251 Hospital of PLA during Jan.2014-Dec.2015,and then were divided into control group (53 cases) and observation group (57 cases) according to random number table.Both groups received routine therapy.Control group was additionally given Dopamine hydrochloride injection or Dobutamine hydrochloride injection as cardiotonic on the basis of routine therapy.Observation group was additionally given Levosimendan injection with initial dose of 10 μg/kg+0.9％ Sodium chloride injection 50 mL,ivgtt (10 min),and then with micro pump infusion at the rate of 0.1 g/(kg· min) for continuous 24 h.Both groups were treated for continuous 7 d.Clinical efficacies of 2 groups were observed,and the levels of left ventricular function indexes (PER,PFR,LVEF,LVSF) level,serum marker (NT-proBNP) and galectin-3 (Gal-3) before and after treatment,the occurrence of ADR was recorded.RESULTS:Two cases were withdrawn from the study due to death (one case in each group).Finally,a total of 108 cases were included,involving 52 cases in control group and 56 cases in observation group.Clinical total response rate of observation group (94.64％) was higher than that of control group (86.54％),but without statistical significance (P＞0.05).Before treatment,there was no statistical significance in left ventricular function indexes or serum markers levels between 2 groups (P＞ 0.05).After treatment,the levels of left ventricular function indexes were improved significantly in 2 groups,and LVEF and LVSF of observation group were significantly higher than those of control group,with statistical significance (P＜0.05).NT-proBNP and Gal-3 of 2 groups were decreased significantly,and the observation group was significantly lower than the control group,with statistical significance (P＜0.05).No obvious ADR was found in 2 groups during treatment.CONCLUSIONS:Leosimendan in the treatment of ALHF have the similor clinical efficacy with dopamine,but helps to strengthen the left heart pump function,reduce heart failure markers levels with good safety.
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Objective:To evaluate the clinical effect of the revaseularization procedure for immature teeth with necrotic pulp and apical periodontitis.Methods:12 immature teeth with necrotic pulp and apical periodontitis in 12 patients(9-13 years old) were treated with revascularization protocol.The patients were followed up for 21-24 months.Results:All teeth were cured.4 types of responses were observed:①Increased thickening of the canal walls and continued root maturation;②Severe calcification of the canal space beneath MTA;③Partial pulp canal obliteration;④Continued root development with an open apex.Conclusion:Revascularization procedure may the formation of the root apex but not in all the cases.Excessive deposition of hard tissue might caused calcification in canal space.
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Objective To investigate the effects of amino acid combined with Salvia miltiorrhiza injection on fetal growth restriction.Methods 122 pregnant women diagnosed with fetal growth restriction were chosen,and they were divided into study group(amino acid+Salvia miltiorrhiza injection) and control group(amino acid).The course of treatment was 7d,and the patients were treated for 3 treatment courses.The clinical efficacy,weight increment,uterine height increment,abdominal circumference increment,fetal biparietal diameter (BPD) increment,femur length (FL) increment,umbilical cord blood flow S/D value and neonatal weight between the two groups were compared.Results The total effective rate in the study group was 84.13%,which was higher than 67.79% in the control group (χ2=4.484,P<0.05).In the study group,the body weight gain,the uterine height increment,abdominal circumference increment were (523.21±103.72)g,(2.88±0.52)cm and (3.19±0.75)cm,respectively,which were significantly higher than those in the control group(t=2.215,4.402,2.328,all P<0.05).The uterine height increment and abdominal circumference increment in the intermediate stage of the study group were (2.14±0.27)cm and (2.89±0.52)cm,respectively,which were significantly higher than those of the control group,and the differences were significant(t=1.503,2.097,all P<0.05).In the study group,the BPD increment and FL increment at prophase and intermediate stage were (2.69±1.23)mm and (2.24±0.97)mm,(2.61±1.28)mm and (2.19±1.28)mm,respectively,which were significantly higher than those in the control group (t=2.053 and 2.244,2.038 and 2.128,all P<0.05).The S/D values of umbilical cord blood flow in early stage,middle stage and anaphase of the study group were (2.33±0.19),(2.46±0.17) and (2.12±0.14),which were significantly lower than those of the control group (t=4.040,2.9817 and 4.418,all P<0.05),the differences were statistically significant.The neonatal weight of the study group was (2 576.18±621.04)g,which of the control group was (2 384.09±428.76)g,and there was significant difference between the two groups(t=1.999,P<0.05).Conclusion Amino acid combined with Salvia miltiorrhiza injection can effectively improve the clinical indicators of pregnant woman and fetal growth restriction,with good clinical effects.
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Objective To explore the effect of telomerase reverse transcriptase(TERT) gene transfected bone marrow stem cell(BMSC)on the memory function and hippocampal CA1 region synaptic plasticity in vascular dementia rat.Methods A total of 60 rats were randomly divided into the negative control group(group A),model group(group B),conventional BMSC group(group C) and transfected BMSC group(group D).The related indicators in each group were detected by using the Morris maze test,RTPCR and Western blot respectively.Results The escape latency period in the group C and group D was significantly longer than that in the group B,which in the group D was significantly longer than that in the group C.Compared with the group A,the expressions of brain-derived neurotrophic factor(BDNF)mRNA,TERT mRNA,SYP mRNA and protein in the group B,group C and group D were significantly decreased.The synaptic cleft arrange in group A was clear with more SYN positive ceils.The synaptic cleft in the group D was clearer,and the number of SYN positive cells was close to that in group A.Conclusion TERT transfected BMSC has obvious therapeutic effect on vascular dementia rats and its mechanism may be related to the promotion of BDNF,TrkB expression and the improvement of synaptic plasticity.
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Objective To evaluate the change of left ventricular diastolic function and investigate the relation between left ventricular diastolic function and disease activity in rheumatoid arthritis (RA) without clinical manifestations of heart diseases. Methods Seventy consecutive active RA in-patients without clinical manifestations of heart disease were enrolled, while the control group was recruited from outpatient health physical check-up center and consisted of 60 age- and sex-matched healthy subjects. Cardiac related parame-ters were determined by echocardiography and the correlation between left ventricular diastolic function and the disease activity indexes were evaluated. Chi-square test, t test, Pearson or Spearman′s correlation test and Stepwise backward linear regression analysis were used for statistical analysis. Results RA patients had lower mitral inflow E/A ratio (1.2±0.4, 1.5±0.4, P<0.01), higher E/Em ratio (9.6±3.7, 7.8±2.0, P<0.01), longer isovolumetric relaxation time(IVRT)[(64±16) ms,(58±16) ms, P<0.05] than control group. Whilst, RA patients had higher pulmonary venous inflow A wave velocity-time integral (ArVTI) and A wave duration (DAr)[3.2±0.7,(2.8±0.6) cm; 117±11,(102±9) ms, P<0.05]. Moreover, the E/Em was positively corre-lated with C-reactive protein(CRP)(r=0.581, P<0.01), DAS28(r=0.456, P<0.01). Anti-CCP level was also associated with Em and early diastolic pulmonary venous inflow peak velocity(PVD)(r=-0.359, P<0.05;r=-0.305, P<0.05). In addition, multivariate analysis also revealed that there was linear regression relation-ship between E/Em and CRP, DAS28(t=3.266, P=0.002; t=2.949, P=0.005). Conclusion The study has revealed that left ventricular diastolic function is impaired in RA patients and the left ventricular diastolic function parameters is associated with the disease activity indexes. These results suggest that the decline of left ventricular diastolic function is associated with the inflammation activity in RA patients without clinical manifestations of heart disease.
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Objective To study the effects of broad-spectrum antibiotics, including the third generation Cephalosporin and Carbapenems, on intestinal commensal bacilli in rats with sepsis. Methods Ninety-one SD rats were randomly divided into normal control group, scald group, scald group with 3 days of ceftriaxone treatment, scald group with 9 days of ceftriaxone treatment, scald group with 3 days of imipenem treatment, scald group with 9 days of imipenem treatment, and sepsis group, sepsis group with 3 days of ceftriaxone treatment, sepsis group with 9 days of ceftriaxone treatment, and sepsis group with 3 days of imipenem treatment, sepsis group with 9 days of imipenem treatment. All the animal were subjected to 30% Ⅲdegree scald injury on their back, followed by endotoxin challenge with a dose of 20mg/kg 24 hours after the burn injury. The animals were treated with intraperitoneal injection of ceftriaxone (60mg/kg, q12h) or imipenem (60mg/kg, q12h) 24h after the second hit with endotoxin. At the end of the treatment, bacilli in the colon were cultured. The number and variety of the bacteria were also determined. Results The antibiotics obviously reduced the number of colibacilli(P
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Objective To study the effects of broad-spectrum antibiotics, including the third generation Cephalosporin and Carbapenems, on intestinal enterococci in septic rats. Methods Ninety-one SD rats were randomly divided into normal control group, scald group, scald group with 3 days of ceftriaxone treatment, scald group with 9 days of ceftriaxone treatment, scald group with 3 days of imipenem treatment, scald group with 9 days of imipenem treatment, and sepsis group, sepsis group with 3 days of ceftriaxone treatment, sepsis group with 9 days of ceftriaxone treatment, and sepsis group with 3 days of imipenem treatment, sepsis group with 9 days of imipenem treatment. All the animal were subjected to 30% TBSA Ⅲ degree scald injury on their back, followed by endotoxin challenge with a dose of 20mg/kg 24 hours after the burn injury. The animals were treated with intraperitoneal injection of ceftriaxone (60mg/kg, q12h) or imipenem (60mg/kg, q12h) 24h after the second hit with endotoxin. At the end of the treatment, intestinal enterococci in colon were cultured. The number and variety of the bacteria were also determined. Results The number of enterococci in the gut of the septic rats was significantly increased after the second hit with endotoxin, exceeding that of the rats in normal control group and scald group(P