ABSTRACT
Objective To explore the safety and efficacy of resistance training (RT) and aerobic exercise (AT) for low back pain during pregnancy. Methods From June to October, 2015, 34 women gestated 20-23 weeks with low back pain accepted resistance training and aero-bic gymnastics exercises twice a week for 12 weeks. They were measured with blood pressure and body mass index (BMI), and assessed with Visual Analogue Scale (VAS) of pain, Self-rating Anxiety Scale (SAS) and Oswestry Disability Index (ODI) before and after treatment. They were investigated the injury and symptoms with questionnaire. Results No musculoskeletal injuries and groin bleeding occurred. Symptoms, such as dizziness and pelvic pain were infrequent (1.5%). The BMI increased (t=-5.791, P<0.001) and the blood pressure did not obviously change (t=1.441, P=0.159). The muscle strength (t=-5.081, P<0.001) and endurance (t=-5.019, P<0.001) increased, and the scores of VAS (t=-5.179, P<0.001), ODI (t=-5.206, P<0.001) and SAS (t=-5.024, P<0.001) significantly improved after treatment. Con-clusion The resistance training combined with aerobic training program during pregnancy is safe and efficacious for pregnant women with low back pain.
ABSTRACT
Objective To construct the wt-p53's eukaryotic expression vector pE6/p53/GFP that was controlled by the radiation induced promoter and research its functions.Methods Radiation response element E6 was synthesized by gene synthesis.The wt-p53 cDNA sequence was prepared from pcDNA3.1(+)/p53 plasmid by PCR.IRES2-EGFP report gene segment was prepared from double cistron expression vector IRES2-EGFP by enzyme digestion.After sequenced and identified,the recombinant plasmid was transformed into H1299(p53-/-)cell with Lipofectamine 2000,and the cell lines in stable expression was screened by G418.In the H1299(p53-/-)cell transfected with the recombinant plasmid or without,wt-p53 mRNA expression was analyzed by RT-PCR,the p53 expression by Western blot when exposed to 4 Gy 8 MV X ray for 0,3,8,12,24,36 h or when exposed to 0,1,2,4,8 Gy 8 MV X ray for 12 h.The cell cycle of H1299(p53-/-)cell transfected stably with the recombinant vector was analyzed by flow cytometry after exposed to 4 Gy 8 MV X ray.Results The recombinant pE6-p53/GFP plasmid had been constructed correctly and the expression of p53 gene in the transfected H1299 cell lines had been determined.After 4 Gy X ray radiation,the expression of wt-p53 protein had a significant rise.The transfected H1299 cell lines stopped in G1 stage after radiation and their cloning efficiency decreased notably.Conclusion We had constructed successfully the recombinant pE6/p53/GFP plasmid that was regulated by radiation induced response element E6.This provides experimental data for radiation-gene therapy of non-small lung cancer.
ABSTRACT
Objective To study the effects of irradiation-induced wt-p53 positive feedback circuit on cell life cycle,apoptosis rate and irradiation sensitivity of adenocarcinoma of lung in vitro.Methods The therapeutic group pE6R4-p53-EGFP/H1299 cells and the other 3 control groups H1299(p53-/-) ,pE6-p53EGFP/H1299 and pR4-p53-EGFP/H1299 cells were irradiated with 8-Mev electron beam generated by a linear accelerator at a dose rate of 400 cGy/min and a source-skin distance(SSD) of 100 cm,12 h exposure to irradiation.The cell life cycle and the rate of apoptosis were analyzed by FCM.Mean lethal dose(Do) and sensitive enhancement ratio(SER) index were calculated from the irradiation dose-survival curve.Results In 12 h post 4-Gy 8MV-X-ray irradiation,FCM analysis showed that most cells in therapeutic group were arrested at G0/G1 stage(75.13?1.42) %,compared with the 3 control groups(38.47?0.87) %,(62.57? 0.76) %and(51.23?2.41) %,respectively.After irradiation,the cell apoptotic rate in each group was higher than that in the cells without irradiation.The apoptotic rate in the therapeutic group was(23.73? 0.21) %,which was 5.69,1.51 and 2.57 folds respectively higher in the 3 control groups(4.17?0.12) %,(15.67?0.32) %and(9.23?0.15) %.Do values were 0.91,1.073 and 1.413 Gy respectively in pE6R4-p53-EGFP/H1299,pE6-p53-EGFP/H1299 and H1299 cells.The SER,derived from Do values,was 2.63 and 1.34,respectively.Conclusion Irradiation up-regulates wt-p53 gene expression,regulates cell cycle and induces cell apoptosis.Thus this positive feedback circuit increases the sensitivity of lung adenocarcinoma to irradiation.