ABSTRACT
Objective To evaluate the reporting item quality of randomized controlled trials of Chinese herbal medicine as an adjunctive therapy for advanced non-small cell lung cancer. Methods The terms including Chinese medicine, Chinese herbal medicine, randomized controlled trials, and non-small cell lung cancer were searched in Chinese and English databases by computer systems to collect relevant literatures. Based on the CONSORT 2010 (consolidated standards of reporting trials 2010), the reporting item quality of the abstracts and main text in eligible papers was evaluated. Results Ninety-five eligible studies were identified from 692 potential eligible articles. None reported all of abstract and main text items in CONSORT 2010. Only 4.12％could be identified as the randomized trial in the title. More than 40％of reports showed the scientific background or rationale in the abstract but not in the main text. Three (3.16％) eligible reports defined the primary or secondary outcome measures. None reported complete information of subjects throughout the clinical trial process. Results A total of 95 eligible papers were collected, of which 0 papers reported complete abstracts and text entries according to the CONSORT. The titles of 4.12％papers were identified as randomized trials. More than 40％of the papers only reported the study purpose or hypothesis in the abstract. 3.16％of the papers reported primary and secondary efficacy index entries, and 0 papers reported complete information of subjects from enrollment to included data analysis sets. Conclusion There is a serious problem of items shortage in the literatures of randomized controlled clinical trials on Chinese herbal medicine as an adjunctive therapy for advanced non-small cell lung cancer. The randomized controlled clinical trials of Chinese herbal medicine as an adjunctive therapy for advanced non-small cell lung cancer should be reported based on the CONSORT to improve the quality of trials.
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Objective:To investigate the protein expression level of lysosome-associated transmembrane protein 4β-35 (LAPTM4B-35) and matrix metalloproteinase-9 (MMP-9) in gastric cancer (GC) and to determine the relationship of LAPTM 4B-35 or MMP-9 expres-sion with clinicopathological characteristics and prognosis of patients with GC. Methods:The messenger RNA (mRNA) expression level of LAPTM4B-35 and MMP-9 was detected by real-time quantitative polymerase chain reaction, while the protein expression level of LAPTM 4B-35 and MMP-9 in GC tissues was analyzed by immunohistochemistry in formalin-fixed samples from 152 patients with GC, and data analysis was performed using statistical methods. Results:The mRNA expression level of both LAPTM4B-35 and MMP-9 in HGC-27, SGC 7901, and MGC803 GC cell lines was higher than that in the GES-1 normal gastric mucosa cell line. LAPTM 4B-35 and MMP-9 proteins mainly localized in the cytoplasm of GC cells. The positive LAPTM4B-35 expression ratio in GC tissues was 50.7％, while the MMP-9 positive ratio was 36.8％. LAPTM4B-35 and MMP-9 were positively related to gastric cancer. Lauren's classification and T stage were identified as the relative factors of LAPTM4B-35 expression, while tumor size and N stage were identified as the rela-tive factors of MMP-9 expression in GC tissues. Cox analysis indicated that the degree of differentiation, N stage, LAPTM 4B-35 expres-sion, and MMP-9 expression were independent prognostic indicators for patients with GC. Conclusion:LAPTM4B-35 and MMP-9 play important roles in the aggressiveness of GC, and LAPTM4B-35 and MMP-9 may be promising indicators for the prognosis of patients with GC.
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The standard therapy for relapsed/refractory Hodgkin lymphoma (RR-HL) consists of salvage chemotherapy followed by high-dose chemotherapy and autologous stem cell transplantation (ASCT). Salvage therapy is not obviously superior to commonly used regimens such as BEAM, CBV, and IGEV. Functional imaging with 18F-fluoro-2-deoxy-D-glucose positron emission tomogra-phy scanning is a critical predictor of the outcome after the completion of salvage chemotherapy and before ASCT. Meanwhile, re-duced-intensity conditioning allogeneic stem cell transplantation may induce a strong response in some patients with relapsing or pro-gressing HL after ASCT. In this study, we reviewed some problems in hematopoietic stem cell transplantation for treating RR-HL.
ABSTRACT
EpigeneticsreferstoheritablealterationsingeneexpressionthatdonotinvolvetheDNA coding sequence itself.Epigenetic regulation plays an important role in gene expression,DNA replication,rep-aration and reconstitution. Thus, the abnormity of epigenetic regulation may lead to different diseases, especially cancer.SNF5 ,which can suppress tumor significantly,is one of the core components of SWI/SNF chromosome remodeling complexes.EZH2,a histone methyltransferase,is closely and complicatedly related to cancer and has significant effect on tumorigenesis.There are many studies reporting the effects of epigenetic regulation factors-SNF5 and EZH2 on different tumors as well as the interrelation at present.In the present paper,the advances in the research on these two epigenetic regulation factors in the carcinogenesis are reviewed.