Comparing multiplex and multiplex real-time polymerase chain reaction with traditional blood culture in bacterial detection among patients with septicemia

Aims@#This study was aimed to test the specificity of primers and probes with target genes by using multiplex PCR and multiplex real-time PCR methods. These methods were compared with traditional blood culture methods in detecting five bacteria causing sepsis, including Acinetorbacter baumannii, Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus.@*Methodology and results@#A total of 587 blood samples from patients diagnosed with sepsis and septic shock were collected at Thanh Nhan Hospital, Hanoi, Vietnam. Each sample was divided into three parts for bacterial culture, multiplex PCR and multiplex real-time PCR to detect the similarity of the two PCR methods with the bacterial culture method. Conditions in multiplex PCR and multiplex real-time PCR were optimized to ensure the successful amplification of target genes. Results showed that the primers and probes were tested completely specific to the target genes and using multiplex PCR and multiplex real-time PCR techniques could detect five pathogens causing sepsis, including A. baumannii, K. pneumoniae, P. aeruginosa, E. coli and S. aureus.@*Conclusion, significance and impact of study@#Both multiplex PCR and multiplex real-time PCR methods have high similarities with the culture method, showing potential in the application of bacteria detection in sepsis.

The quality and use of water for living in several ecological regions of Viet Nam \r\n', u'\r\n', u'

Background: A research on the quality and the use of water for living in order to discover solutions to ensure the supply of fresh water is urgent and necessary. It is also a basis to help policy makers to implement the right plan in supplying water for each region. Objectives: This research aims to evaluate the quality and the usage of water for living in several ecological regions of Viet Nam. Subjects and method: A descriptive, cross sectional study conducted in 7 chemical and physical indices and 2 microbiological indices in 1479 samples of waters that are used in 4 ecological regions of Vietnam. Results:The whole of the country has a high coverage of clean water (88.4%), but only 22.8% of sample meets the requirements. Especially, most of the samples do not meet the microbiological requirements. Conclusion: In general, the pollution level of the sources of water is at a medium level. The related factor that affects the clean water standard is the polluted sources under 10m nearby the polluting site.

Electrocardiogram in 86 patients with the arterial hypertension

A study was carried out on 86 hypertensive inpatients with ages of 30-80 year old in internal department of Th¸i Nguyªn Central General Hospital during 4-12/1998, who received the electrocardiogram. The results has shown that the Sokolow-lyon indicator changed frequently (73.25%), the pathological T wave changed in 61.63% of patients, the reduction of ST wave accounted for 40.69%. There were some electrocardiogram indicators insignificantly changed in hypertension including alphas angle, eletrocardiac axis and cardiac position.

Preliminary investigation on diseases of the elderly patients in the internal diseases department of Thai nguyen Hospital

This study included 448 patients older than 60 years during 1992 with the following characteristics: sex: 55.58% male; 44.42% female. Geographic repartition: 57.14% from country side: 42.86% from town. Ethnic repatition: 94.64% Kinh; 3.35% Tay; 1.12% Nung and 0.89% (3 other ethnic minorities). Diseases of: gastro- intestinal tract: 27.23%. Respiratory tract: 26.56%. Cardiovascular system: 21.65%. Nervous system: 10.26%. Urinary- genital tract: 6.47%. Blood system: 2.9%. Others: 3.34%.