ABSTRACT
Objective To establish the method for quality control of ethyl-acetate parts of Ferula sinkiangesis. Methods HPLC was used to detect the contents of ferulic acid, farnesiferol A and farnesiferol C. Waters XTerra RPC18 column (250 mm×4.6 mm, 5 μm) was used; acetonitrile-0.1% phosphoric acid was as mobile phase with gradient elution; flow rate was 1.0 mL/min; detective wavelength was 324 nm; temperature was 30℃; sample volume was 10 μL ResultsFerulic acid, farnesiferol A, and farnesiferol C showed a good linear relationship range from 0.05– 1.0 mg/mL, 0.132–2.64 mg/mL, and 0.118–2.36 mg/mL, respectively. The average recovery rates were 99.34%, 98.96% and 99.24% respectively. The contents of ferulic acid, farnesiferol A and farnesiferol C were 33.4, 76.5, 72.3 mg/g respectively.Conclusion The method was simple, accurate and repeatable, with good repeatability and stability, which can be used for the quality control of ethyl-acetate parts ofFerula sinkiangesis.
ABSTRACT
Objective To establish a high-throughput detection of medicine on DPPH free radical scavenging activity;To analyze and preliminarily screen the anti-oxidant activity part of Ferula sinkiangensis. Methods Taking DPPH free radical scavenging rate of ascorbic acid as positive control, and IC50 as the evaluating criterion of DPPH free radical scavenging capability, oxidation resistance of different polar parts (petroleum ether part, ethyl acetate part, methyl alcohol part, and water part) of Ferula sinkiangensis was evaluated and screened. Results Different polarity parts of Ferula sinkiangensis DPPH radical scavenging of IC50 were:petroleum ether part 3252.22 μg/mL, ethyl acetate part 36.22 μg/mL, methyl alcohol part 32.22 μg/mL, water part 2643.38 μg/mL, and positive control group ascorbic acid 27.16 μg/mL. Conclusion The ethyl acetate and methyl alcohol parts of Ferula sinkiangensis were effective anti-oxidant activity site to eliminate DPPH free radicals. In this study, a simple, sensitive, and high-throughput detection method of DPPH radical scavenging assay was established to provide reference for the anti-oxidation medicine detection screening.