ABSTRACT
Objective:To research the best processing method for Strychni Semen with children's urine by orthogonal test. Methods:L9(34) orthogonal test was used with the types of children's urine, dosage of children's urine, soaking time and drying temperature as the influencing factors,HPLC was applied to determine the contents of brucine and strychnine as the indices to obtain the comprehensive weighted score. ANOVA was used to optimize the processing technology of Strychni Semen with children's urine. Results:The soaking time showed significant effect on the content of brucine and strychnine, while the other three factors had no significant effect. The best processing technology was A1B1C3D3. Conclusion:The optimum processing of Strychni Semen with children's urine is as follows:3-year-old children's urine with 3-fold amount is used to soak for 10 d,and the drying temperature is 90 ℃. The method is stable and reliable, which can be used for the new processing technology of Strychni Semen.