Massa de forragem, composição morfológica e valor nutritivo de capim-braquiária submetido a níveis de sombreamento e fertilização / Herbage mass, morphological composition and nutritive value of signalgrass, submitted to shading and fertilization levels

Características produtivas e nutricionais de Brachiaria decumbens, submetida a condições de sombreamento (sol pleno, 20% e 70% de sombreamento), sem e com uso de fertilização (80 kg.ha-1 de N e K2O e 60 kg.ha-1 de P2O5), foram avaliadas no delineamento de blocos casualizados, com quatro repetições. As parcelas sombreadas foram alocadas em um sistema silvipastoril, enquanto aquelas a pleno sol foram avaliadas em um monocultivo de B. decumbens. O sombreamento severo promoveu reduções de 54% na massa seca verde, 59% na massa seca total e 58% na densidade volumétrica de forragem. Aumentos da massa de forragem e da densidade volumétrica em resposta ao fertilizante foram observados somente nas condições de sol pleno e sombreamento moderado. A adubação não influenciou nas características nutricionais da forragem, mas o sombreamento severo promoveu aumentos de 20 e 51% nos teores de clorofila e de proteína bruta, respectivamente. Reduções dos teores de fibra em detergente neutro e fibra em detergente ácido foram observadas com o sombreamento. Os teores de lignina e a digestibilidade in vitro da matéria seca não variaram com nenhum fator estudado (médias de 6,0 e 60,9%, respectivamente). A adubação de B. decumbens com dose moderada de NPK é recomendada para dosséis cultivados no sol pleno e sombreamento moderado, mas deve ser evitada em pastos severamente sombreados, pois não reflete aumento de massa de forragem nem melhorias de seu valor nutricional.(AU)

Productive and nutritional traits of Brachiaria decumbens, submitted to shading conditions (full sun, 20% and 70% shading) and fertilization levels (without or with 80 kg.ha-1 of N and K 2 O and 60 kg.ha-1 of P 2O5), were evaluated in a randomized complete block design, with four replications. The shading were allocated in a silvopastoral system, while full sun was obtained in an exclusive B. decumbens pasture. The severe shading reduced the green dry mass in 54%, total dry mass in 59% and the forage bulk density in 58%. Increases in the forage mass and forage bulk density in response to fertilization were observed only in full sun and moderate shade. The nutritional traits did not vary with fertilization, but the chlorophyll and crude protein contents increased 20 and 51% with severe shading, respectively. The neutral detergent fiber and acid detergent fiber reduced with shade. The lignin content and in vitro dry matter digestibility did not vary with factors (average values of 6.0 and 60.9%, respectively). Fertilization with moderate dose of NPK is recommended to B. decumbens in full sun and moderate shading, but should be avoided in severely shaded pasture, because not reflected in improvement of forage mass and its nutritional traits.(AU)

The conservation status of the tuco-tucos, genus Ctenomys (Rodentia: Ctenomyidae), in southern Brazil

The goal of conservation biology should be related to the preservation of species and also to the evolutionary and ecological processes that were responsible to form them and that are still acting. We review the conservation status of the species of tuco-tuco (Ctenomys torquatus, C. lami, C. minutus, and C. flamarioni) from southern Brazil, and relate these data to the geological history of a particular area in that region, the Coastal Plain of the States of Rio Grande do Sul and Santa Catarina. The implications of the data on these species from the Southeastern Brazil are also discussed in relation to the evolution and risk of extinction of these subterranean rodents.

O objetivo da biologia da conservação deve estar relacionado com a preservação das espécies e também com os processos evolutivos e ecológicos que foram responsáveis por sua formação e que continuam ocorrendo. Este trabalho revisa o status de conservação das espécies de tuco-tuco (Ctenomys torquatus, C. lami, C. minutus e C. flamarioni) do sul do Brasil, assim como a relação entre estas informações e a história geológica de uma região de especial importância, a planície costeira dos Estados do Rio Grande do Sul e Santa Catarina. São também discutidas as implicações do conhecimento acumulado sobre as espécies de tuco-tuco no sul do Brasil em relação aos aspectos evolutivos e a ameaça de extinção que estas espécies de roedores subterrâneos sofrem.

Risk of hepatitis B virus transmission by diagnostic hysteroscopy

Few data are available in the literature concerning the efficacy of standard hysteroscope disinfection procedures to prevent hepatitis B transmission. The aim of the present study was to determine the risk of hepatitis B virus (HBV) transmission during hysteroscopy among anti-HBc-seropositive women. Serum and hysteroscopic samples were collected from 62 women after diagnostic hysteroscopy. All samples were tested for serologic HBV markers. Polymerase chain reactions (PCR) were carried out to amplify regions C and S of the viral genome and only samples amplified by both pairs of primers were considered to be positive. Anti-HBc was repeatedly reactive in 48 (77 percent) of 62 serum samples, and HBsAg was detected in 8 (13 percent). At least one HBV serologic marker was found in 49 (79 percent) samples. Only one sample was HBsAg positive and anti-HBc negative. HBV-DNA was detected by PCR in 7 serum samples but in only 3 hysteroscopic samples obtained just after hysteroscopy. It is noteworthy that high levels of anti-HBc IgM were detected in one HBsAg-negative patient who showed an HBV-DNA-positive hysteroscopic sample. An elevated sample/cut-off ratio for anti-HBc IgM suggests recent infection and reinforces the need for testing for HBsAg and anti-HBc before hysteroscopy, since acute hepatitis B can be clinically asymptomatic. Viral DNA was not detected in any hysteroscopic samples collected after washing and disinfecting procedures with glutaraldehyde. We conclude that HBV-DNA can be found in the hysteroscope soon after hysteroscopy, but standard disinfecting procedures are effective in viral removal.

Fertility of unilaterally vasectomized rats and ultrastructure of sperm granuloma.

Os testiculos, epididimos e ductos deferentes de ratos albinos, com vasectomia unilateral apresentaram histologia normal. Os granulomas de esperma aumentaram progressivamente de tamanho durante os 280 dias de experiencia e as eletromicrografias de sua parede mostram celulas gigantes de corpo estranho enfileiradas fagocitando fragmentos de espermatozoides.Setenta e cinco por cento dos ratos unilateralmente vasectomizados mantiveram a sua capacidade reprodutora