ABSTRACT
The Rosemary [Rosmarinus officinalis L.], a well-known medicinal and culinary herb, was studied to compare the terpenic profile of different extracts obtained from dry and fresh herb. There were studied the volatile oil extracted by hydro distillation from dry plant, the hydroalcoholic extracts obtained from fresh respectively dry plant and the glycerol macerate obtained from fresh plant, by GC-MS using headspace injection. The separated compounds were identified using a MS spectra library. The quantitative determination was performed by normalization respectively by calibration curve method for 1,8-cineole, alpha-pinene and D-limonene. The main separated compounds were alpha-pinene, 1,8- cineol, camphene, camphor, D-limonene and cymene. A significant difference was observed between the 4 samples volatile profiles. 1,8-cineole was found major component of the essential oil [VO-21.39%] and glycerol macerate [GM- 35.60%], while and alpha-pinene was detected as the main constituent of the two tinctures [T-46.05%; MT-31.93%]. The highest 1,8-cineol content, determined by calibration curve method, was found in the volatile oil, while the fresh plant hydroalcoholic extract was richer in alpha-pinene and D-limonene
ABSTRACT
The purpose of our study was to identify the phenolic substances of two varieties of Chrysanthemum balsamita [balsamita and tanacetoides] and to measure the overall antioxidant activity. The phenolic compounds were determined by HPLC. The evaluation of the polyphenolic content was performed by colorimetric analysis. The antioxidant activity was measured by three in vitro assay models: the DPPH, the silver nanoparticles antioxidant capacity [SNPAC] and EPR radical detection. Using HPLC-MS analysis, phenolic acids, flavonoids and flavonoid aglycone were detected. The highest antioxidant activity was showed by Chrysanthemum balsamita var. balsamita, while the lowest for the Chrysanthemum balsamita var. tanacetoides extract, in accord with the polyphenolic content. The results show that Chrysanthemum balsamita var. balsamita might be a source of antioxidant flavonoids, especially rutin and isoquercitrin
ABSTRACT
The purpose of this study was to compare the polyphenol profile of different Rosemary [Rosmarinus officinalis] extracts obtained from dry and fresh herb and the evaluation of their antioxidant effect. There were studied the polyphenols from hydroalcoholic extracts [1:5- dry plant:solvent] obtained from fresh respectively dry plant and the gemmotherapic extract obtained from fresh plant [1:20- dry plant: solvent]. The polyphenol profile was evaluated by UV-Vis spectrophotometry, TLC and HPLC. The total polyphenol respectively flavonoids content were determined by UV-Vis spectrophotometry. The antioxidant effect was evaluated by DPPH, ABTS, FRAP, CUPRAC and silver nanoparticle [SNP] methods. The hydroalcoholic extract obtained from fresh plant contains the highest concentration of total polyphenols, [0.601mg/ml rosmarinic acid], total flavonoids, [0.270mg/ml luteoline] and rosmarinic acid [0.350 mg/ml]. The less concentrated is the gemmotherapic extract, but also the extraction ratio is higher than the hydroalcoholic extracts. The high content in polyphenols of the mentioned hydroalcoholic extract was confirmed by highest values of antioxidant activity: 39.1ml [DPPH], 7.7 ml [ABTS], 698mM ET/100 ml [FRAP], 1947 mM ET/100 ml [CUPRAC], 4570mM ET/100 ml [SNP]. These differences in the polyphenols profiles show the importance of use the fresh plants for obtaining the good quality extracts