ABSTRACT
Abstract Multidrug-resistant microorganisms are of great concern to public health. Genetic mobile elements, such as plasmids, are among the most relevant mechanisms by which bacteria achieve this resistance. We obtained an Escherichia coli strain CM6, isolated from cattle presenting severe diarrheic symptoms in the State of Querétaro, Mexico. It was found to contain a 70 kb plasmid (pMEX01) with a high similarity to the pHK01-like plasmids that were previously identified and described in Hong Kong. Analysis of the pMEX01 sequence revealed the presence of a blaCTX-M-14 gene, which is responsible for conferring resistance to multiple β-lactam antibiotics. Several genes putatively involved in the conjugative transfer were also identified on the plasmid. The strain CM6 is of high epidemiological concern because it not only displays resistance to multiple β-lactam antibiotics but also to other kinds of antibiotics.
Subject(s)
Animals , Cattle , Plasmids/genetics , Cattle Diseases/microbiology , Drug Resistance, Bacterial , beta-Lactams/pharmacology , Escherichia coli/drug effects , Escherichia coli/genetics , Escherichia coli Infections/veterinary , Anti-Bacterial Agents/pharmacology , Plasmids/metabolism , beta-Lactamases/genetics , beta-Lactamases/metabolism , Microbial Sensitivity Tests , Escherichia coli Proteins/genetics , Escherichia coli Proteins/metabolism , Escherichia coli/metabolism , Escherichia coli Infections/microbiology , MexicoABSTRACT
La litotripsia extracorporal por ondas de choque ha sido usada con éxito para fragmentar cálculos renales y ureterales en humanos desde 1980. En este artículo se informa de un tratamiento de litotripsia estracorporal en un perro con un cálculo no inducido implantado en la vejiga, usando un generador de ondas de choque experimental hecho en el país. Después de dos tratamientos el perro eliminó todos los fragmentos y las arenillas del cálculo. Este artículo puede representar la base para el desarrollo de la litrotipsia extracorporal para animales usando equipos relativamente baratos y fáciles de construir
Subject(s)
Dogs , Animals , Lithotripsy/veterinary , Dog Diseases/therapy , Ampicillin , Acetaminophen , Urinary Bladder Calculi/veterinary , Trimethoprim, Sulfamethoxazole Drug Combination/administration & dosageABSTRACT
El presente artículo describe los resultados de las primeras pruebas in vivo en roedores y perros para determinar el daño causado a diferentes órganos y tejidos expuestos al generador de ondas de choque MEXILIT I.