Changes in protein fractions, trypsin inhibitor and proteolytic activity in the cotyledons of germinating chickpea

The chickpea seed germination was carried out in 6 days. During the period it was observed a little variation on total nitrogen contents, however the non protein nitrogen was double. A decrease of 19.1 and 20.6 in relation to total nitrogen was observed to the total globulin and albumin fractions, respectively. The gel filtration chromatography on Sepharose CL-6B and SDS-PAGE demonstrated alterations on the distribution patterns of the albumin and total globulin fractions between the initial and the sixth day of germination suggesting the occurrence of protein degradation in the germination process. The assay for acid protease only appeared in the albumin fraction with casein and chickpea total globulin as substrates, whereas the former was more degraded than the latter, however the transformations detected in the protein fractions appear indicated that others enzymes could be acting during the process. The trypsin inhibitor activity had a little drop after six day of germination indicating a possible increase on the digestibility of the proteins.

Efeito do tempo de difusao na determinaçao de amonia urinaria pelo metodo de Conway / Effect of the diffusion time on the determination of urinary ammonia throug the Conway method

Estudou-se o efeito do tempo de difusao na determinaçao de amonia urinaria pelo metodo de Conway. Em uma mesma amostra de urina de 24 horas foi determinada a amonia urinaria utilizando-se os seguintes tempos de difusao: 3,6,16,24,30 e 48 horas. a maior quantidade de amonia urinaria foi obtida nos tempos de difusao de 30 e 48 horas. Deste modo e recomendado o controle rigoroso das condiçoes de coleta, acidificaçao e armazenamento da urina, o uso sistematico de curvas de calibraçao com tempo de difusao fixo e predeterminado, levando-se tambem em conta a temperatura ambiente e a diluiçao da urina, para que toda a amonia seja absorvida no menor tempo de difusao