Enfermedad de paget ósea: breve revisión de la literatura a propósito de un caso / Paget ́s bone disease: case report and brief literature review

La osteítis deformante o enfermedad de Paget es un trastorno óseo, crónico, poco frecuente en Chile, que puede afectar típicamente a pacientes después de los 55 años. Su etiología es probablemente multifactorial: actualmente se considera como posiblemente implicados factores ambientales y genéticos.La mayor parte de los pacientes son asintomáticos. Los diagnósticos habitualmente se realizan en base a hallazgos radiológicos. Cuando existen síntomas, suelen ser dolor y/o deformidad ósea. Pueden comprometerse uno o varios huesos.Ante su sospecha clínica, la exploración diagnóstica requiere de al menos el estudio con radiografías simples de las lesiones sospechosas, cintigrama óseo y parámetros de actividad metabólica ósea; recomendándose los niveles séricos de fosfatasas alca-linas, por su bajo costo y elevada disponibilidad.Las metástasis óseas condensantes son un importante diagnóstico diferencial a con-siderar.Sobre el tratamiento, se recomienda el uso de fármacos antirresortivos como terapia específica, y el uso de ortesis y terapia física.El seguimiento es clínico y con biomarcadores de recambio óseo.

Osteitis-deformans or Paget's disease is a chronic bone disorder uncommon in Chile, with a typical presentation after age 55. Its etiology is probably multifactorial: It is actualy considered that environmental and genetic factors are mainly involved.Most patients are asymptomatic. Diagnoses are usualy performed based on radio-logical findings. When symptoms exist, they are pain and/or bone deformity. One or several bones can be affects.If it ́s suspect, the diagnostic exploration requires at least the study with x-rays of the suspicious lesions, bone scintigraphy and parameters of bone metabolic activity, being recommended the serum levels of alkaline phosphatases, for its low cost and high availability.Condensing bone metatases have to be considered like an important differential di-agnosis.For the treatment, the use of antiresorptive drugs as specific therapy is recommend-ed, and the use of orthotics and physical therapy.Follow-up is made with clinical findings and with biomarkers of bone turnover.

Identificación y tipificación de Haemophilus influenzae mediante PCR múltiple / Use of multiplex PCR for detection and identification of Haemophilus influenzae

Se diseñó una estrategia metodológica para la detección simultánea entre Haemophilus influenzae serotipo b (Hib) y no tipificable (HiNT), así como para discriminar entre H. influenzae, Moraxella catarrhalis y Streptococcus pneumoniae. El estudio se realizó en una recolección de 64 cepas, las cuales se sometieron a amplificación por PCR utilizando ADN genómico o directamente el cultivo bacteriano como plantilla. La PCR se realizó en dos fases: 1) PCR triple para discriminar entre H. influenzae y M. catarrhalis empleando oligonucleótidos específicos para los genes del ARNr 16S y de la neumolisina (Ply) para identificar S. pneumoniae; 2) PCR doble para la identificación simultánea de Hib y HiNT, utilizando oligonucleótidos específicos de los genes cap y hmw. Se logró la amplificación del gen ARNr 16S en las 64 cepas de H. influenzae, sin obtenerse amplificación en las cepas control. La secuencia Bex A se amplificó en 29 de 32 cepas de Hib, y la secuencia HMWA se amplificó en 29 de 30 cepas de HiNT. La amplificación con los oligonucleótidos específicos para M. catarrhalis y S. pneumoniae no mostró productos con ninguna cepa de H. influenzae. Estos resultados permiten proponer la aplicación de esta herramienta metodológica para discriminar entre M. catarrhalis y S. pneumoniae, e identificar H. influenzae directamente a partir de muestras clínicas.

La Encuesta de Estudiantes de Nivel Medio y Medio Superior de la Ciudad de México: noviembre 2003. Prevalencias y evolución del consumo de drogas

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Abstract: Introduction: Research focused on drug consumption in schools has been developed in México since 1976 and has allowed for a constant monitoring of this behavior in the population. The National Institute of Psychiatry Ramón de la Fuente (INP) and the Public Education Administration (SEP) have been the pionering institutions in these efforts, where other interested institutions and states add their contributions to the assessment of the problem in this social sector. Due to the need of developing local preventive programs to reduce drug consumption, several regions of the country have carried out, in a first stage, situational diagnoses of drug consumption. These data, in addition to other community information sources, have helped to enhance efforts in terms of prevention. To date, the main investigations related to students are the Fourth National Survey on Addictions, the National Survey from the National System for the Integral Family Development, regional surveys with young students from 7th to 91 grades (Queretaro; Ríoverde, San Luis Potosí; Sinaloa; Tamaulipas and Ciudad Guzmán, Jalisco.), from 101 to 12th grade (same states, except for Ciudad Guzman and the study done in Guanajuato whose results have not been published yet) and college (Ríoverde). The main findings for adolescents and youths obtained from these studies show an increase in drug consumption, specially for alcohol, cannabis and metamphetamines, although the general index of consumption remains steady since 2001, specially in the case of cocaine. There have also been changes in men and female contributions to the consumption index, giving similar prevalences for alcohol and tobacco in both. Regional variations have been observed, where drug consumption is higher in more urbanized cities. However, new generations are more affected by this phenomenon regardless of the level of urbanization of the place where they live. Also, as it has been previously established, being in a scholastic environment is a protective factor against drug consumption, beacause consumption is higher among adolescents who do not study, and it is a differential factor that protects men more than women. As a consequence, these sources and different students' surveys point out that the probability of drug consumption increases when a minor is working. Additionally, studies report that an early consumption onset for tobacco and alcohol, mainly before 13 years old, increases the possibility of consuming other drugs. This fact is important because several reports on the literature show that age of onset for consuming these drugs is becoming earlier. It is relevant to consider that drug consumption is not an isolated factor. It is known that some precursors for drug consumption are the same for other behaviors, such as sexual intercourses without protection, antisocial, delinquent behaviors or suicide attempt. In behalf of this, prevention programs must be designed in an integral way considering the global environment of adolescents, and not just focused on drug consumption. From this point of view, results from the survey on drug use carried out among students in Mexico City in autumn, 2003, are presented. This survey is the most recent diagnosis about this problem in Mexican adolescents, and keeps the methodological standards of previous surveys. Objective: The aim of the present work is to give a recent and complete view about this problem and prevention opportunities for adolescents of Mexico City and the whole country. Materials and method: The study comprised a randomized sample of 10,659 students from Mexico City, with a two-stage design (school-group), and stratified (from 71 to 9 grades, and from 10 to 123 grades, technical and normal), where the last selection unit was the scholar group. Data is representative for delegation and educational level, and the design is similar to those previously applied in schools by the INP and SEP. The reviewed sample frame was obtained from the registrations to the 2001-2002 school period of SEP A randomized sample was obtained for each county and educational level. Regarding estimations precision, calculated non-response index was 15%, with an absolute error average of 0.004, and a design effect equaling 2. The confidence intervals obtained were generated by the STATA program, version 7.0. The mean-age of the subjects was 14.6 years (12-22 years), 50.5% of the sample were men and 49.5% were women. The indicators of drug consumption included in the questionnaire are the same used in similar studies and are the same used by the WHO. In addition to drug consumption and related problems, several behaviors were assessed among adolescents, such as suicide attempt, level of depression, eating risk behaviors and some features of their sexual behavior. Interviewers were trained to obtain the most reliable information from the adolescents and to keep confidentiality. A total of 23 interviewers and five supervisors participated in the study. Results: First of all, a slightly increase in drug consumption was found in the last three years, from 14.7% to 15.2%, which is not significative. Nevertheless, there are significant changes in the consumption of specific drugs. There is a significant increase in cannabis consumption, while inhalants and tranquilizers remain steady and cocaine use showed a slightly decrease. By sex, it was observed that drug consumption in women has increased, while in men it remains steady or has even decreased a little. In this context, drug preferences remain very similar to those reported three years ago, where cannabis occupied the first place, followed by inhalants, tranquilizers and cocaine (with similar levels of consumption between the last three ones). The drugs of preference among women are tranquilizers. With respect to legal drugs, alcohol consumption has increased, while tobacco consumption remains similar to the reported in the survey of 2000. A significant change in these drugs, as mentioned above, is that their consumption is almost the same among men and women. Talking about alcohol abuse, a 2% increase was observed, which indicates 23.8% of the adolescents had consumed at least five drinks per occasion during the month previous to the study. In regional terms, there were also changes. The most affected delegations are Azcapotzalco, Venustiano Carranza, Miguel Hidalgo and Cuauhtemoc. In the last survey, the most affected delegations were Gustavo A. Madero and Coyoacan. This is very important information, because the SEP implemented several prevention strategies in these two delegations in order to reduce drug consumption. Considering the results of the present study, it can be concluded these efforts were successful. Even though results will be presented in a specific publication, according to the adolescents' evaluation of the brochure given to each student at the end of the application, we can say they were very positive, as 15% of the adolescents indicated they had quitted smoking and a similar percentage reported a decrease in their tobacco consumption. Another 15% mentioned they had used help telephone lines, and more than 60% shared the content of the brochure with their families. Most of them have kept the brochure for future occasions and have also lent it to friends. Discussion: The data about drug consumption is similar to the data of other national studies reporting that consumption of medical and illegal drugs and tobacco, is steadier on the whole, although there are changes in specific drugs consumption and by sex. Meanwhile, the prevalence of alcohol consumption has been increasing, even when its abuse remains stable. For prevention, it is important to consider the new location of the problem (most affected delegations), and to use crime indicators and other delinquent behaviors, to share prevention efforts in the most affected areas of the city. Another interesting result is the low level of risk perception for tobacco and alcohol consumption, which are important precursors for the consumption of other drugs, especially if there is an early consumption onset for these substances. In the context of drug consumption associated with other adolescents' problems, suicide attempt is the most frequent situation reported by the participants (16%) and it is even more frequent than drug consumption. Although this situation seems to be quite problematic, adolescent population in scholastic environments is the least affected in comparison to those that quit or stop studying. On the other hand, the information obtained about the brochure "What's up with your life?" is encouraging for prevention practices, and the presence and growth of these problems makes it important to consider that the process of obtaining information as an integral part of the diagnosis may also be used as an opportunity to reach adolescents with information or materials created for them. Finally, it is important to point out that prevention must be applied during childhood and not only during adolescence. To reach this goal, it is important to include all possible human resources. The point is simple: the possibilities to obtain better results under this conception are greater, because the interaction with children and their complete integration at home facilitate this prevention task. During childhood, the human being is more receptive to this kind of interventions and it is simpler to work with the families. So, when children grow up, it will be easier to communicate with them as adolescents and to give them all the support they require in their identity formation. Even when the work is focused on the longer term, the results will be better and we will be able to offer better options to the new generations from the construction of an adequate prevention culture.