ABSTRACT
Objective To determine the encapsulation efficiency of glycyrrhetinic acid(GA) liposomes and to study the release of GA from the liposomes in vitro.Methods GA liposomes were prepared by ethanol injection.The unencapsulated glycyrrhetinic acid and liposomes were separated by sephadex gel G-50,and the encapsulation efficiency was detected by HPLC.The release behavior of the drug was studied by the third method for dissolution issued in Chinese pharmacopoeia(published in 2005).Results The encapsulation efficiency of liposomes was 91.61%,and the in-vitro drug-release curve was accorded with Higuchi equation.Conclusion The GA liposomes have high encapsulation efficiency and sustained-release effect.
ABSTRACT
Objective To prepare and characterize glycyrrhetinic acid cationic liposomes.Methods Liposomes were prepared by ethanol injection technique.An orthogonal test was utilized to optimize the formulation and preparation of glycyrrhetinic acid liposomes.The unencapsulated glycyrrhetinic acid and liposomes were separated by Sephadex gel G-50,the encapsulation efficiency was detected by HPLC.The morphological examination of glycyrrhetinic acid liposomes was performed using transmission electron microscopy.The particle size and Zeta potential of the liposomes were measured.The release rate of glycyrrhetinic acid from liposomes was tested.Results The liposomes with spherical or ellipsoidal shape and better stability featured the encapsulation efficiency of(91.61?1.16)%,the mean partical size of(141?10)nm,and Zeta potential of(35.9?5)mV.The in vitro release kinetics were consistent with Higuchi equation.The stability of glycyrrhetinic acid liposomes was better.Conclusion The selected formulation and preparation technic of glycyrrhetinic acid liposomes could be rational,stable,and with a sustained feature in vitro release.