ABSTRACT
Objective To investigate the molecular characteristics of genome sequence of Thelazia callipaeda(T.cp).Meth-ods The obtained T.cp genome assembling data were annotated by using a combination of ab initio gene by softwares,Gene-Mark and GeneID,and the homology of the experimentally confirmed genes was predicted by software GeMoMa.The results were integrated by software EVM to predict all genes of genome.The obtained genes were annotated in the common public data-base and three dedicated databases(CAZyme,TCDB and PHI),respectively.Results The Scaffolds and Contigs gene struc-ture of T.cp genome(79.34 Mb)was analyzed,and a total of 6 333 genes were obtained.The sequence search was conducted in the public databases using BLASTx,of which 97.85％of the genes could be annotated.The genes annotated in the NR database were the most(98.69％),and those enriched in the KEGG pathway were the least(50.50％).The functional genes were blasted by KOG database and totally 4 517 genes were found.The three special databases(CAZyme,TCDB and PHI)were used to an-notate all the genes,and 136,139 and 1 498 genes were assigned respectively,and the number of genes in the PHI database was the largest.In the cytochrome proprietary database,238 cytochrome P450 genes were predicted.Conclusion We have pre-liminarily revealed the T.cp genome structure characteristics and annotation information,and totally 6 333 genes are obtained.
ABSTRACT
We conducted prediction and analysis for secretory proteins from Thelazia callipaeda at Genome Scale based on the previous full genome annotation.The software SignalP,TMHMM,big-PI Predictor,MEME,Protcomp and SecretomeP were combined to process the prediction of the secretome of Thelazia callipaeda.The analyses of secretory proteins by GO function enrichment,KEGG pathway,and statistics of domains were performed.Results showed that totally 259 secretory proteins were found in Thelazia callipaeda genome and the amino acid lengths of secretory proteins were mainly concentrated between 100 to 700 aa exclusively.GO function analysis of secretory proteins indicated that they were enriched in the secreting pathways and in the interactions with host.The results of KEGG metabolism secretory proteins suggested that some of them contributed to drug metabolism and glutathione metabolism.And domain analysis suggested that most of them were glycoside hydrolase,contributing to sugar metabolism.Around 126 secretory proteins had antigenicity of B-cell epitope.In summary,we found that secretory proteins in Thelazia callipaeda were most small proteins,which were involved in sugar metabolism and antioxidative activity,facilitating Thelazia callipaeda to invade the hosts and play a key role in the parasitic course.