ABSTRACT
<p><b>OBJECTIVE</b>To study and establish the fingerprints of Herba Senecionis Scandentis by RP-HPLC and LC-MS.</p><p><b>METHOD</b>C18 column was used in the HPLC separation. The mobile phase was composed of acetonitrile and 0.03% phosphoric acid with gradient elution. The flow rate was 1.0 mL m x min(-1) and detection wavelength was 254 nm.</p><p><b>RESULT</b>Under this selected chromatographic condition, good HPLC fingerprints of Senecio scandens were obtained.</p><p><b>CONCLUSION</b>HPLC fingerprint facilitate the evaluation of the quality of Herba Senecionis Scandentis.</p>
Subject(s)
Asteraceae , Chemistry , Chromatography, High Pressure Liquid , Methods , Drugs, Chinese Herbal , Chemistry , Reproducibility of Results , Spectrometry, Mass, Electrospray Ionization , MethodsABSTRACT
<p><b>OBJECTIVE</b>To isolate the constituents of Senecio scandens and determine their structures.</p><p><b>METHOD</b>Componds were isolated and purified by silica gel column chromatography and the structures were identified by spectroscopic methods.</p><p><b>RESULT</b>Nine compounds were isolated as lupenone (1) , oleanane (2) , beta-sitosterol (3) , daucosterol (4) , adonifoline (5) , phydroxy benzeneacetic acid (6) , 2-(1,4-dihydroxy-cyclohexanyl) -acetic acid (7), hyperoside (8), linarin (9).</p><p><b>CONCLUSION</b>These compounds were obtained from S. scandens for the first time except 4 and 6.</p>