Inhibitory effects of LDL-ACM complex on subcutaneous implanted tumors in nude mice / 中国病理生理杂志

AIM: In order to evaluate the applicable value of LDL as a targeted vehicle for chemotherapeutic agents, we investigated and compared the inhibitory effects of LDL-ACM complex and free ACM on nude mice's subcutaneous implanted tumors derived from gastric cancer cell lines, SGC-7901 and NKM-45. METHODS: LDL-ACM complex was prepared and the tumor model of nude mice was established by subcutaneous implantation of SGC-7901 and NKM-45. Then, the groups of nude mice developed subcutaneous implanted tumors were received either LDL-ACM complex or free ACM. Subsequently, the tumor size, weight and leukemia cell counts were measured and the rates of tumor-inhibition and the survival were compared among the groups. RESULTS: The inhibitory effects of LDL-ACM complex on the tumors, especially on SGC-7901 implanted tumors were much more obvious than that of free ACM. It was also indicated that the action of LDL-ACM complex was mediated by LDL receptor. CONCLUSION: These results showed that LDL-ACM complex had significant inhibitory effects on the implanted tumors and the effect might be mediated by LDL receptor.

Construction of human prostate-specific membrane antigen expression vector and identification of tissue specificity / 中国病理生理杂志

AIM:To construct a prostate-specific expression vector of promoter and enhancer of human prostate-specific membrane antigen(PSMA).METHODS:The promoter and enhancer of PSMA were amplified by PCR separately.The two segments were cloned into the expression vector pGL3-Basic,a prostate-specific expression vector pGL3-PSMP-PSME was constructed.The vector was transfected into prostatic carcinoma cell PC-3M and four kinds of non-prostatic carcinoma cells by lipofectamine.The activity of luciferase of transfected cells and tissue specificity of vector was examined at 48 hours after transfection.RESULTS:With DNA sequence of the vector pGL3-PSMP-PSME,the segment of clone was proved correct.The activity of luciferase of the pGL3-PSMP-PSME was expressed distinctly in PC-3M and was not expressed in other nonprostate cell lines.CONCLUSION:The prostate-specific expression vector was constructed successfully.It lays foundation for studying target gene therapy of the prostate cancer.