ABSTRACT
Abstract Nematophagous fungi from the feces of water buffalo and soil from southeastern Mexico were isolated, and their in vitro predatory activity against Haemonchus contortus infective larvae (L3) (HcL3) was assessed. The fungi were isolated by sprinkling soil or feces on water agar plates. Six series of 10 Petri dishes containing a 7-day-old culture of each fungus and a series without fungi as the control were prepared. Five hundred HcL3 were added to each plate. The plates were incubated at room temperature. The average of recovered HcL3 was considered to estimate the larval reduction rate. Four nematophagous fungi isolates corresponding to Arthrobotrys oligospora, var microspora (strains 4-276, 269 and 50-80) and one identified as A. oligospora,var. oligospora (isolates 48-80) were obtained from water buffalo feces. From the soil, five isolates were isolated; three corresponded to A. musiformis (Bajío, Yumca and Macuspana isolates), and two isolates were identified as A. oligospora (Comalcalco and Jalapa de Méndez isolates). The predatory activity of isolates from water buffalo feces ranged between 85.9 and 100%. Meanwhile, the fungi from the soil ranged between 55.5 and 100% (p≤0.05). The nematophagous fungi obtained could have important implications in the control of parasites of importance in the livestock industry.
Resumo Fungos nematófagos das fezes de búfalo de água e do solo no sudeste do México foram isolados, e a atividade predatória in vitro contra larvas infectantes de Haemonchus contortus (L3) (HcL3) foi avaliada.Os fungos foram isolados por aspersão de solo e de fezes em placas de agar água. Foram preparadas seis séries de 10 placas de Petri contendo uma cultura de 7 dias de idade de cada fungo e uma série sem fungos como controle. Quinhentos HcL3 foram adicionadas a cada placa. As placas foram incubadas à temperatura ambiente. O número médio de HcL3 recuperadas foi considerado para estimar a taxa de redução larval. Quatro isolados de fungos nematófagos corresponderam a Arthrobotrys oligospora, var microspora (estirpes 4-276, 269 e 50-80) e um isolado identificado como A. oligospora, var. oligospora (isolados 48-80 de fezes de búfalo de água. Do solo, dos cinco isolados três corresponderam a A. musiformis (Bajío, Yumca e Macuspana isolados), e dois isolados foram identificados como A. oligospora (isolados de Comalcalco e Jalapa de Méndez). A atividade predatória de isolados de fezes de búfalo de água variou entre 85,9 e 100%. Enquanto isso, os fungos do solo variaram entre 55,5 e 100% (p≤0,05). Os fungos nematófagos obtidos podem ter importantes implicações nesse controle de parasitos de importância na indústria pecuária.
Subject(s)
Animals , Male , Female , Soil/parasitology , Buffaloes/parasitology , Feces/parasitology , Haemonchus/physiology , Haemonchus/isolation & purification , Haemonchus/classification , MexicoABSTRACT
The aim of this study was to evaluate the productivity and profitability of two commercial sources of progesterone therapy as part of a hormonal induction treatment in a heifer dual purpose herd. A second objective was to determine the productivity and profitability of a recombinant bovine somatotropin (ST) application during induced lactation. Forty four Holstein heifers x Zebu crosses were used. Four treatments and two control groups were evaluated. For economic analysis all components of total cost (TC) of the companies were considered, further there was a simulation which was designed for three natural lactations after the hormonal induction, based on two scenarios in which different body weights were considered as different periods. Artificial induction of lactation yielded a 100% success rate in the four groups of animals with no statistical differences between them (P > 0.05). The best cost-benefit ratio (CBR) was recorded by CIDR treatment without ST. Although the four treatments showed economic viability, those without ST had a better ratio (B / C) than those supplemented with ST. As for the net present value (NPV), the benefits updated showed that the profits were higher when ST was not applied and, mainly, in the CIDR treatment. The internal rate of return (IRR) reveals that most of the treatments have a yield above 20%.
Los objetivos del presente trabajo fueron: 1) evaluar la productividad y rentabilidad de dos fuentes comerciales de progesterona como integrantes de un tratamiento lactoinductor en vaquillas de reemplazo de un hato de doble propósito y 2) determinar la productividad y rentabilidad de la aplicación de somatotropina bovina recombinante (ST) durante la lactación inducida. Se usaron 44 novillonas producto de cruzas Holstein x Cebú. Se evaluaron cuatro tratamientos y se manejaron dos grupos testigo. Para el análisis económico se consideraron todos los componentes del costo total (CT) de las empresas, adicionalmente se realizó una simulación en la que se proyectó para tres lactaciones naturales posteriores a la lactoinducción, basadas en dos escenarios en los que se consideraron diferentes pesos corporales como diferentes periodos. En los cuatro grupos de animales lactoinducidos, el 100% de las vaquillas respondió al tratamiento sin diferencias estadísticas entre ellas (P > 0.05). La mejor relación beneficio-costos (RBC) la registró el tratamiento CIDR sin ST. Si bien los cuatro tratamientos presentaron viabilidad económica, los tratamientos sin ST presentaron mejor relación (beneficio/costo) que aquellos tratamientos que sí fueron complementados con ST. En cuanto al valor actual neto (VAN), los beneficios actualizados de todos los años muestran que las utilidades que generaron los tratamientos fueron superiores cuando no se aplicó ST y, principalmente, en el tratamiento con CIDR. La tasa interna de rentabilidad (TIR) revela que la mayoría de los tratamientos tienen una rentabilidad arriba del 20%.
ABSTRACT
Bovine leptospirosis causes high economic losses in cattle mainly due to reproductive failure, as well as representing public health risk. Since the last century, antibody titers against several Leptospira serovars have been detected by the microscopic agglutination test (MAT) in Mexico. With the exception of very few cases, the presence of serovars causing leptospirosis in cattle and other animal species has not been demonstrated by isolation in Mexico, and in such cases characterization had to be done abroad by complex and slow immunological approaches, by comparison with a number of reference strains. The present study was conducted to perform the molecular characterization of Leptospira isolates by multiple locus sequencing typing (MLST). A hundred and ninety seven sera and kidneys samples were collected immediately after slaughter, from grazing cattle coming from the south-eastern states of Mexico. Anti-Leptospira antibodies were detected by the MAT and kidneys were inoculated into EMJH and Fletcher's specific medium. A seropositivity of 60.4% (119 out of 197), with titers from 1:100 up to 1:3 200 was detected. Four isolates (2.03%), referred as CAL4, CAL6, CAL7 and MOCA45, were characterized by serology, ribotyping and MLST as L. kirschneri serovar Grippotyphosa; L. interrogans serovar Hardjo; L. santarosai serovar Mini and L. santarosai serovar Tarassovi, respectively. With the exception of serovar Hardjo, the three other isolates belong to serovars and species not previously isolated in Mexico. These findings make it necessary to evaluate the potential distribution of such serovars among cattle and their role on animal production and public health.
La leptospirosis bovina causa grandes pérdidas económicas a la ganadería por problemas reproductivos y también es un riesgo de salud pública. En México, desde el siglo pasado se ha registrado la presencia de anticuerpos contra serovariedades de Leptospira por la técnica de aglutinación microscópica (AM), en bovinos y otras especies animales. En muy pocos casos, la enfermedad fue demostrada por el aislamiento de Leptospira, y en tales casos, su caracterización se basó en métodos inmunológicos lentos que requirieron la comparación con cepas de referencia y que fueron realizados fuera de México. En el presente trabajo se realizó la caracterización molecular mediante la secuenciación de locus múltiples (MLST), de aislados de Leptospira obtenidos de riñones de bovinos recolectados en rastro, procedentes de las zonas Golfo y sur de México. Se obtuvieron muestras de suero y riñones de 197 bovinos para realizar la AM, y el cultivo en medios específicos EMJH y Fletcher. Se detectó una seropositividad del 60.4% (119 de 197), con títulos desde 1:100 hasta 1:3,200 y se obtuvieron cuatro aislados de Leptospira (2.03%), denominados CAL4, CAL6, CAL7 y MOCA45. Los aislados fueron caracterizados por serología, ribotipificación y MLST, como L. kirschneri serovariedad Grippotyphposa; L. interrogans serovariedad Hardjo; L. santarosai serovariedad Mini y L. santarosai serovariedad Tarassovi, respectivamente. A excepción de la serovariedad Hardjo, los aislados pertenecen a especies y serovariedades no aisladas anteriormente en la República Mexicana, esto sugiere la necesidad de evaluar su diseminación entre bovinos y su potencial efecto en la producción animal y en la salud pública.