ABSTRACT
Objective To observe the urine analysis test paper evaluation method for the determination of the sensitivity of medical equipment cleaning effect,for cleaning quality evaluation method.Methods To perform standard manual processing equipment in the experiments,the first use of ATP monitoring method and the analysis of urine test paper test mid experiment data,calculate its whether there is relationship and ATP test method,and can instruct the cleaning quality results;Second use visual method,ATP bioluminescence method and the analysis of urine test paper test the end of the experiment data,the analysis to determine the urine test paper measurement indicator cleaning qualified evaluation threshold.Results The ATP test paper method and urine analysis had showed positive correlation,0.30 ≤r≤0.75,and to complete the standard cleaning process 300 pieces of department of gynaecology attractor visual analysis method,ATP bioluminescence method and the analysis of urine test paper measurement inspection group (the -),cleaning the percent of pass was 95.67％(287/300),86.33％(259/300) and 83.37％(251/300) respectively,therefore,urine analysis test paper assay to detect the - was the cleaning threshold quality qualification evaluation,urine analysis test paper test the percent of pass was 14.46％ lower than the visual observation,x2=63.35,P＜0.05.Conclusions Urine analysis test paper test results,test method is simple,low cost,and can be widely used in medical equipment cleaning quality evaluation method of semi-quantitative daily monitoring.
ABSTRACT
Objective To isolate enterovirus 71 from a death children,and analyze whether the neurovirulence was related to the variation of nucleotide and amino acid. Methods Enterovirus 71 was isolated from throat swabs which were colleted from Shandong Linyi People's Hospital. The full length genome was sequenced by amplification with RT-PCR and sequencing of 9 overlapped gene fragments covering full length of the genomes. The nucleotide and amino acid sequenced was aligned by BLAST, Bioedit and MEGA 4. Results A strain of enterovirus 71 was isolated and named as SDLY107. The full length was 7405 bp. The results of homology analysis of overall nucleotide sequence showed that strain Fuyang. Anhui. P. R. C/17.08/2 had highest homology (98.6%)with strain SDLY107, and the homology was 80.0% between strain SDLY107 with prototype strain BrCr/70,and 86. 5% between strain SDLY107 with nerve strain MS/87. Phylogenetic analysis showed that the phylogeny was close between SDLY107 with some isolated strains from Chinese Mainland, such as Beijing, Henan, Guangxi, Sbenzhen, Lanzhou, Fuyang, Chongqing and Zhejiang strains, which was clustered for C4 subtype. The results of amino acid sequence analysis showed that there were 2 mutations, E947D and K1873R, for strain SDLY107. Conclusion SDLY107 belonged to C4 subtype, amino acid mutations E947D and K1873R of which may be relevant to the pathogenicity of EV71.