ABSTRACT
ABSTRACT Introduction: The Pilates is a training method that aims to improve body awareness for movement re-education, bringing muscular and mental balance. It is believed that these benefits can effectively improve the flexibility of female university aerobics students. Methods: 90 female college students in the optional aerobics course were selected and divided into two groups. Data from the experimental and control groups were evaluated for comparison before and after the experiment. The aerobic exercises of the two groups of female university aerobics students were scored, aiming to compare and explore which training method was more effective. Results: The baseline time and pace score in the Pilates intervention was changed from 2.35±0.71 to 2.55±0.76, a movement set changed from 3.86±0.98 to 4.01±1.07, movement performance from 1.67±0.61 to 1.72±0.57, and foot skills from 1.40±0.48 to 1.41±0.50. The mean aerobic movement performance score changed from 62.45 to 80.53, an increase of 18.08 points. Conclusion: The Pilates training can effectively improve the aerobic performance of female university students. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.
RESUMO Introdução: O Pilates é um método de treinamento que visa trabalhar a consciência corporal para reeducação do movimento, trazendo equilíbrio muscular e mental. Acredita-se que esses benefícios possam efetivamente melhorar a flexibilidade das estudantes universitárias de aeróbica. Objetivo: Avaliar os efeitos do treinamento baseado em pilates sobre o desempenho esportivo de estudantes universitárias. Métodos: 90 estudantes universitárias femininas do curso opcional de aeróbica foram selecionadas e divididas em dois grupos. Os dados do grupo experimental e do grupo de controle foram avaliados para comparação antes e depois do experimento. Os exercícios aeróbicos dos dois grupos de estudantes universitárias femininas de aeróbica foram pontuados, visando a comparação e explorar qual o método de treinamento mais eficaz. Resultados: O valor de tempo e ritmo basal na intervenção com pilates foi alterado de 2,35±0,71 para 2,55±0,76, o conjunto de movimentos mudou de 3,86±0,98 para 4,01±1,07, o desempenho do movimento de 1,67±0,61 para 1,72±0,57, e as habilidades com os pés de 1,40±0,48 para 1,41±0,50. A pontuação média do desempenho do movimento aeróbico mudou de 62,45 para 80,53, um aumento de 18,08 pontos. Conclusão: O treinamento de Pilates pode efetivamente melhorar o desempenho de estudantes universitárias femininas de aeróbica. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.
RESUMEN Introducción: Pilates es un método de entrenamiento que pretende trabajar la conciencia corporal para la reeducación del movimiento, aportando equilibrio muscular y mental. Se cree que estos beneficios pueden mejorar eficazmente la flexibilidad de las estudiantes universitarias de aeróbic. Objetivo: Evaluar los efectos del entrenamiento basado en pilates en el rendimiento deportivo de estudiantes universitarias. Métodos: Se seleccionaron 90 estudiantes universitarias del curso optativo de aeróbic y se dividieron en dos grupos. Se evaluaron los datos del grupo experimental y del grupo de control para compararlos antes y después del experimento. Se puntuaron los ejercicios aeróbicos de los dos grupos de estudiantes universitarias de aeróbic, con el objetivo de comparar y explorar qué método de entrenamiento era más eficaz. Resultados: La puntuación inicial de tiempo y ritmo en el experimento de pilates pasó de 2,35±0,71 a 2,55±0,76, la del conjunto de movimientos pasó de 3,86±0,98 a 4,01±1,07, la del rendimiento de los movimientos pasó de 1,67±0,61 a 1,72±0,57, y la de las habilidades con los pies pasó de 1,40±0,48 a 1,41±0,50. La puntuación media del rendimiento de los movimientos aeróbicos pasó de 1,67±0,61 a 1,72±0,57, y la de las habilidades con los pies de 1,40±0,48 a 1,41±0,50. La puntuación media del rendimiento del movimiento aeróbico pasó de 62,45 a 80,53, lo que supone un aumento de 18,08 puntos. Conclusión: El entrenamiento con Pilates puede mejorar eficazmente el rendimiento aeróbico de las estudiantes universitarias. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.
ABSTRACT
Hepatocellular carcinoma is one of the most prevalent malignancies and a leading cause of cancer-related mortality worldwide. However, the therapies to prevent hepatocellular carcinoma are still limited and the emergence of drug resistance leads to the development of new anti-cancer drugs and combinational chemotherapy regimens. Our study was aimed to explore the anticancer effects of the essential oil extract (EEEO) from Euphorbia esula which has been widely used in traditional Chinese folk medicine and possessed potential cytotoxic effects in several human tumor cells. However, the mechanisms of EEEO-induced anti-proliferation and apoptosis have not been completely elucidated. In this study, EEEO was prepared by hydro-distillation and the main chemical component of EEEO was identified by GC-MS. HepG2 cells were treated with EEEO in vitro and then evaluated with respect to proliferation, apoptosis, and levels of reactive oxygen species (ROS) and apoptotic proteins. Our studies showed that EEEO decreased cell viability, elevated ROS levels, and induced apoptosis of HepG2 cells in a concentration- and time-dependent manner. Furthermore, Bcl-2 was down-regulated, while Bax was up-regulated in HepG2 after EEEO treatment. These results suggest that EEEO induced apoptosis of HepG2 cells and indicate that this apoptosis might be mediated by the mitochondrial pathway.
ABSTRACT
Siegesbeckia pubescens (SP) has been used as a traditional medicine for the treatment of and inflammatory diseases.However,the activities of SP against hepatocellular carcinoma and the related mechanisms remain unclear.The present study aimed to examine the effects of the essential oil of SP (SPEO) on the proliferation of hepatocellular carcinoma cells and the possible mechanisms.The growth inhibition of HepG2 cells was analyzed by MTT assay.Hoechst 33258 and fluorescence microscopy were utilized to observe the nuclear morphological changes of apoptotic cells.Flow cytometry was used to detect cell apoptosis and cell cycle.The expressions of the target proteins were detected by Western blotting.The results showed that SPEO obviously inhibited the proliferation of HepG2 cells in a dose-dependent manner.SPEO activated a series of apoptotic proteins in HepG2 cells,increasing expression levels of Bax,caspase-3 and caspase-9,and decreasing the bcl-2 expression level.SPEO displayed promising anti-hepatocellular carcinoma activities in vitro,partly by inducing apoptosis in HepG2 cells through activating the mitochondrial pathway.