The role of complement C5a receptor in DPSC odontoblastic differentiation and in vivo reparative dentin formation / 国际口腔科学杂志·英文版

Therapeutic dentin regeneration remains difficult to achieve, and a majority of the attention has been given to anabolic strategies to promote dentinogenesis directly, whereas, the available literature is insufficient to understand the role of inflammation and inflammatory complement system on dentinogenesis. The aim of this study is to determine the role of complement C5a receptor (C5aR) in regulating dental pulp stem cells (DPSCs) differentiation and in vivo dentin regeneration. Human DPSCs were subjected to odontogenic differentiation in osteogenic media treated with the C5aR agonist and C5aR antagonist. In vivo dentin formation was evaluated using the dentin injury/pulp-capping model of the C5a-deficient and wild-type mice. In vitro results demonstrate that C5aR inhibition caused a substantial reduction in odontogenic DPSCs differentiation markers such as DMP-1 and DSPP, while the C5aR activation increased these key odontogenic genes compared to control. A reparative dentin formation using the C5a-deficient mice shows that dentin regeneration is significantly reduced in the C5a-deficient mice. These data suggest a positive role of C5aR in the odontogenic DPSCs differentiation and tertiary/reparative dentin formation. This study addresses a novel regulatory pathway and a therapeutic approach for improving the efficiency of dentin regeneration in affected teeth.

Immediate placement and immediate provisionalization of ITI implants in maxillary non-restorable single teeth. A prelimenary report

OBJECTIVE: This prospective study was conducted to examine the outcome of Sandblasted Large Grit Acid Etched [SLA] surface, solid screw implants placed immediately into extraction sockets and loaded within one hour of implant placement to achieve immediate replacement of non-restorable single maxillary teeth. PATIENTS and METHODS: Thirty-five ITI implants in thirty-one patients were placed and immediately restored with a non-occluding provisional acrylic crown. Porcelain fused to metal crowns were cemented six weeks later. All 35 implants achieved osseointegration as demonstrated by stability and function at the placement of the final un-splinted crown. The healing process was uneventful and without adverse events. There were no reported peri-implant mucosal complications. The prosthetic procedures occurred without intervening incident of abutment loosening or fracture. DISCUSSION: Considerable advantages exist to immediate placement and provisiolanization after tooth extraction, soft tissue profiles can remain stable and alveolar bone loss can be avoided. The rationale for replacing maxillary molars is debatable. CONCLUSIONS: Immediate placement of ITI dental implants following tooth extractions and immediately provisionalized is a viable treatment option