ABSTRACT
Objective:To investigate the effect and potential mechanism of serum exosome-derived cirC_0009362 on the osteogenic differentiation of human bone marrow mesenchymalstem cells (hBMSCs) .Methods:Serum samples from patients with osteoporosis (OP) were collected and exosomes were isolated. The expression level of circ_0009362 in exosomes was detected by qRT-PCR. hBMSCs osteogenesis was induced and the expression of circ_0009362 and miR-29b-3p was detected. The interaction between circ_0009362 and miR-29b-3p was detected by dual luciferase reporter assay. Alkaline phosphatase (ALP) kit was used to detect ALP activity and alizarin red (ARS) staining was used to detect calcium deposition.Results:Compared with healthy control group, the expression of circ_0009362 in serum exosomes of OP patients was increased, and the expression of circ_0009362 was decreased after inducing hBMSCs osteogenesis (all P<0.05) . The ALP activity and the percentage of calcium deposition in hBMSCs were decreased by exosomes, and this effect was achieved by secreting circ_0009362. The effect of exosomes was partially offset by circ_0009362 expression in knockdown exosomes (all P<0.05) . The expression of miR-29b-3p was increased after inducing hBMSCs osteogenesis ( P<0.05) . Circ_0009362 had a targeting relationship with miR-29b-3p, and exosomes inhibited the expression of miR-29b-3p by secreting circ_0009362. The ALP activity and the percentage of calcium deposition in hBMSCs were promoted by overexpression of miR-29b-3p, which was partially offset by exosomes (all P<0.05) . Conclusion:Serum exosomes of OP patients inhibit the osteogenic differentiation of hBMSCs by secreting circ_0009362 to down-regulate the expression of miR-29b-3p.
ABSTRACT
Objective To systematically review the prednisone-induced test results for the prognosis assessment of acute lymphoblastic leukemia(ALL) in children.Methods Based on the established inclusion and exclusion criteria,studies of prednisone-induced test in evaluating the prognosis of childhood ALL were electronically searched from January 1990 to November 2016 from PubMed,Embase,The Cochrane Library,WanFang,VIP and CNKI data-base.Two independent researchers browsed literature,extracted data and evaluated the literature quality,Meta-analysis was performed by using RevMen5.3 software.Results A total of 17 articles were included.Meta analysis showed that after completing prednisone induced test in children,the 5 year-event-free survival (5 y-EFS) and 8 y-EFS of good response to prednisone (PGR) was better than that of poor response(PPR) group to prednisone [mean deviation (MD) =22.03,95 ％ CI:18.87-25.18,P ＜ 0.000 01;MD =27.90,95 ％ CI:20.94-34.85,P ＜ 0.000 01].The continuous complete remission rate and recurrence rate in 2 groups were compared,and the continuous complete remission of PGR group was high [odds ratio (OR) =4.82,95 ％ CI:2.81-8.29,P ＜ 0.000 01] and the recurrence rate was low (OR =0.26,95 ％ CI:0.11-0.59,P =0.001),and all the differences were statistically significant.There were statistical significance in T cell immune typing,the initial white blood cell count and at day 19(D19) bone marrow(M1,M2) between 2 groups [relative risk (RR) =0.30,95 ％ CI:0.20-0.44,P ＜ 0.000 01;OR =0.11,95 ％ CI:0.08-0.16,P ＜0.000 01;OR =8.53,95％ CI:2.85-25.52,P =0.000 1;OR =0.12,95％ CI:0.03-0.39,P =0.000 5].There was no statistical significance in gender(male) and D19 bone marrow(M3) between 2 groups (OR =0.89,95％ CI:0.62-1.28,P=0.53;OR=0.24,95％CI:0.04-1.44,P=0.12).Conclusion The prednisone induction test is an important index to evaluate the prognosis of children with ALL,and the treatment of prednisone induced test based on selection can improve the prognosis of children.