Polyhydroxyalkanoates degradation affects survival of Pseudomonas oleovorans in river water microcosms

Bacterial survival in natural environments involves the ability of scavenging nutrients and energy sources. Polyhydroxyalkanoates (PHAs) are intracellular polymers that endow bacteria with enhanced survival capabilities in adverse environmental conditions. In this paper we compared survival of Pseudomonas oleovorans wild type and PHA depolymerase mutant strains in natural river waters by using microcosms. Experiments were performed with water samples collected from the Rio de la Plata. The survival of the P. oleovorans strain capable of degrading PHA was higher in raw river water compared to the depolymerase negative mutant strain. Bacterial numbers decreased during the experiment. At the end of the experiment, the difference in the number of cells between wild type and mutant strains was of 3 orders of magnitude. Mutants deficient in PHA degradation are useful to study the importance of reserve polymers in the survival of bacterial species in natural environments. They could also provide an adequate system for the analysis of the role of PHA in the tolerance to physical or chemical stress agents.

Mutantes con alta producción de butanol de una cepa de Clostridium solventogénica aislada de alpechín / [Mutants with high butanol production from a strain of solventogenic Clostridium isolated from olive black-water]

Butanol high producing mutants of a solventogenic Clostridium sp. capable of degrading olive black water, were selected according to ethanol or butanol resistance after treatment with N-methyl-N’-nitro-N-nitrosoguanidine. Mutants were quickly screened from isolated colonies and then characterized in standard culture conditions.