Immunopotentiality of Ayurvedic polyherbal formulations “Saribadi” and “Anantamul Salsa” with augmentation of IgM production and lymphocytes proliferation: A preliminary study

Objective To assess the immunopotentiality of Ayurvedic polyherbal preparations, “Saribadi” and “Anantamul Salsa”. Methods Freshly prepared BALB/c mice splenocytes were cultured with “Saribadi” or “Anantamul Salsa” treatment [doses of 0.25%, 0.50%, 0.75%, 1.00%, 1.50%, 2.00%, 3.00% and 4.00% (v/v)] at 37 °C for 5 days. The immunoglobulin M (IgM) production and lymphocytes proliferation were determined by ELISA and MTT methods, respectively. Endotoxin contamination was assessed by treating the preparations with polymyxin B. Results The doses of “Saribadi” [0.25%, 0.50%, 0.75% and 1.00% (v/v)] significantly increased IgM productions (0.966, 0.728, 0.695 and 0.615 μg/mL vs. control 0.265 μg/mL) and lymphocytes proliferation [absorbance 0.311, 0.394, 0.372 and 0.334 optical density (OD) vs. control 0.162 OD]. Similarly, the doses of “Anantamul Salsa” [0.50%, 0.75%, 1.00% and 1.50% (v/v)] promoted IgM productions (0.933, 0.919, 0.917 and 0.892 μg/mL vs. control 0.502 μg/mL) and the doses of “Anantamul Salsa” [0.50%, 0.75%, 1.00%, 1.50%, 2.00%, and 3.00% (v/v)] stimulated lymphocytes proliferation (absorbance 0.395, 0.326, 0.440, 0.398, 0.452 and 0.355 OD vs. control 0.199 OD). The activity of “Saribadi” and “Anantamul Salsa” was not retarded by the treatment of preparations with polymyxin B. Conclusions Immunomodulatory activity of “Saribadi” and “Anantamul Salsa” was unveiled for the first time. “Saribadi” and “Anantamul Salsa” possess immunostimulating potential acting through the induction of lymphocyte proliferation and IgM production. These preparations may be useful in strengthening immune responses. However, further cellular and in vivo studies are required.

investigation on the pharmacological activity of an ayurvedic formulation mahadraksharista

In this study an Ayurvedic formulation Mahadraksharista was studied for its pharmacological properties using laboratory mice. There was a constant reduction in food and water intake in Mahadraksharista treated mice, consequently there was also a reduction in urine volume, stool and water content of stool. The Mahadraksharista in a significant extent reduced the intestinal motility. This reduced intestinal motility was further supported by the significant anti-diarrhoeal property of the preparation in castor oil induced diarrhoea test. The formulation markedly increased the latent period of diarrhoea and reduced the purging index value. The Mahadraksharista significantly reduced the onset of sleeping time, but had no effect on the duration of sleeping time

Effect of vasantakusumakar rasa: an ayurvedic antidiabetic drug on central nervous system and gastrointestinal tract

The present study was carried out to investigate some pharmacological effects of Vasantakusumakar Rasa [VKR] on the Central Nervous System [CNS] and the Gastrointestinal [GI] Tract utilizing laboratory mice. The CNS study comprised of Hole cross test, Open field test and Hole board test and the GI study comprised of GI-Motility, Castor oil induced and MgSO[4] induced diarrhoeal tests. VKR showed stimulatory effect in the Hole cross test, Open field test and the Hole board head dipping test. In case of GI-Motility test, VKR decreased the gut movement. In case of Castor oil induced diarrhoeal test the mean latent period increased. There was no prominent effect in MgSO[4] induced diarrhoeal tests. Conclusion from the above tests reveals that VKR has CNS stimulant activity and constipatory effect on the Gastrointestinal Tract