Evaluation of seven assays detecting serum immunoglobulin classes and sub - classes and salivary and faecal secretory IgA against Fasciola excretory / secretory [ES] antigens in diagnosing fascioliasis

Seven assays detecting serum IgM, IgG, IgG1, IgG4, IgA and salivary and fecal excretory IgA against Fasciola excretory/secretory [ES] antigens were evaluated in diagnosing fascioliasis, for cross reactivity with Schistosoma mansoni sera and for the evaluation of the cure of Fasciola infection after treatment. Assays detecting sera IgM, IgGl, IgG4 and IgA against Fasciola ES antigens showed 100% specificity and sensitivity. Assays detecting IgM and IgG showed 98% and 96% sensitivity as well as 100% and 94.6%, specificity respectively. Assays detecting salivary and fecal IgA showed 92% and 96% sensitivity as well as 100% and 100% specificity, respectively. Assays detecting IgM and IgG4 were the best in the evaluation of cure and assays detecting IgG4 and IgA showed the lowest cross-reactivity with sera from S. Mansoni infected patients. So, assays detecting serum IgA, IgG1 and IgG4 against Fasciola ES antigens were highly sensitive and specific for the diagnosis of fascioliasis and assays detecting salivary and fecal IgA were promising and was found to be of a great help in the diagnosis of fascioliasis, especially in epidemiologic studies

role of endobrush in diagnosis of endometrial pathology in perimenopausal uterine bleeding

The value of the Endobrush endometrial cell sampling device in the cytological assessment of the endometrium was compared with dilatation and curettage. 100 women presenting with perimenopausal uterine bleeding were studied by both endometrial cytology and histopathology. The Endobrush sampling procedure caused no pain in 94% and slight pain in 6%. There was no incidence of failed insertion among the 100 cases examined. None of the women developed complications from the use of the instrument. The smears yielded specimens with abundant cells in 76% and moderate number of cells in 24%. None of the smears were acellular. In the 100 patients, 6 [6%] had a histopathological diagnosis of endometrial adenocarcinoma, and 78 [78%] had a histopathological diagnosis of endometrial hyperplasia. The cytology samples obtained with this device were diagnostic of endometrial adenocarcinoma in 5 [83.3%] of these 6 patients and 66 [84.6%] of the patients with endometrial hyperplasia. To lower these false negative results, the combination of office sampling with vaginal ultrasonography is proposed. A negative sampling in the presence of a thickened sonographic endometrial lining warrants additional investigation. The Endobrush cytology sampler is considered a safe and effective screening tool for endometrial adenocarcinoma and hyperplasia. The method is simple, quick and painless and therefore well acceptable to patients and suitable for clinical use

Cytogenetic study of spontaneous abortion

Eighty one abortuses were studied in this work the goal of which is to detect the whole frequency of chromosomal abnormalities among spontaneous abortion. Thirty one cases were successfully cultured and on karyotyping twenty seven cases were found to be of normal chromosomal constitution whereas the remaining four abortuses were of abnormal karyotype including triploid, monosomy X and translocation

Study of the relative frequency of the different types of chromosomal abnormalities among spontaneous abortion

Out of thirty one abortuses successfully cultured, four specimens revealed, under karyotyping, abnormal chromosomal constitution including triploidy, translocation to the short arm of one of the C-group chromosomes [12/2 translocation] X-O monosomy and a ring chromosome. The four abnormalities are represented by figures 2, 3, 4 and 5

Acupuncture analgesia in obstetric surgery in Alexandria. A preliminary report on 19 cases of lower segment caesarean sections and one case of abdominal hysterotomy and stcrilizatoin

Acupuncture analgcsisa was tried in 19 cases of lower segment Caesorean sections and one case of abdominal hysterotomy and sterilization. The technique Proved to be simple, safe and reliable especially when traditional anaesthsia proves to be risk