Mental health status before and after cosmetic rhinoplasty

Background and Objective: Patients with psychological disorders including depression, anxiety and patients with choronic psychological diseases are not suitable for cosmetic rhinoplasty.This study was done to evaluate the mental health status before and after cosmetic rhinoplasty

Methods: This quasi- experimental study was done on 100 women and men with mean age of 26.85 +/- 6.60 which candidated for cosmetic rhinoplasty. Subjects filled out psychological disorder symptom questianare [SCL-90-R] before and 3-6 months after cosmetic rhinoplasty

Results: Women had more symptom [P<0.05] of depression [7.24 versus 10.79], somatization [4.64 versus 7.35], obsessive-compulsive [5.67 versus 8.51], interpersonal sensitivity [5.48 versus 7.52], anxiety [5.26 versus 8.07], symptom distress index [0.58 versus 0.79] and totally positive symptoms [32.29 versus 41.06] in comparison with men. The main effect of time factor showed that there was not significant difference between symptoms of psychological disorder before and 3-6 months after cosmetic rhinoplasty. Interactional effect of gender and time was not significant for each psychological disorder symptom

Conclusion: The differences of deviation of psychological disorder symptoms, before cosmetic rhinoplasty and 3-6 months after cosmetic rhinoplasty were not significant. This finding can denotes existence of psychological symptoms before cosmetic surgery. Cosmetic surgery strengthens psychological symptoms in women the assessment and psychological intermediate with the aim of reducing psychological symptoms especially at time range of 3-6 months should be done

In vitro potential of human bone marrow and umbilical cord vein mesenchymal stem cells to differentiate into insulin producing cells

Mesenchymal stem cells [MSCs] derived from bone marrow are multi potent cells that have the capacity to trans-different!ate into a variety of cell types including insulin islet cells. However the efficiency is low. The aim of this study is to explore the potential of Marrow and Umbilical cord vein MSC to differentiate into functional islet like cells in vitro. BM-MSCs and UC-MSCs were obtained from healthy donors and were cultured. MSCs with high CD90, CD73, CD105, CD44 and very low CD34 and CD45 expression were differentiated into Islet-like cells, under defined conditions. Insulin and c-peptide positive cells were evaluated with immune-florescence and insulin release after glucose challenge was tested by ELISA. QRT-PCR was done to detect expression of insulin, Glut2, Nkx6.1 and Nkx2.2 at mRNA level. Our results showed that only BM-MSC can be differentiated to insulin secreting cells. About 15.8% +/- 2.6 and 13.5% +/- 5.5 of cells were positive for insulin and c-peptide, respectively. Our results revealed that expression of Insulin and Glut2 upregulated 20 fold changes at mRNA level. However they were not functional when treated by different concentration of glucose. Our results showed that only Human BM-MSCs, compared to umbilical cord vein MSCs, are able to differentiate into insulin producing cells in vitro

[Ultra structure of human uterine and oviduct epithelial cells cultivated under the same ulture condition]

Introduction: Extra cellular matrix [ECM] as an important component of cellular microenvironment has a key role in maintaining the differentiated state of cells. Effects of ECM on morphologic differentiation of epithelial cells including those from uterus and oviduct has been shown in past studies in which cellular and hormonal factors have been used in addition to ECM to maintain epithelial cell differentiation. Not much attention has been paid, in these studies; about the ultra structure of cultured cells specially those from oviduct

Objective: The purpose of present study is to cultivate the human uterine and oviduct epithelial cells under the same microenvironment [ECM Gel and DMEM/Ham's F12 medium] and to observe and compare ultra structural characteristics of the cultured cells by transmission electron microscopy [TEM]

Materials and Methods: For this purpose, uterine and oviduct tissue were obtained from patients undergoing total hysterectomy in Emam Khomeini Hospital. Epithelial cells, after being isolated, were cultured on plastic surfaces and the epithelial nature of the cells was confirmed using immunocytochemistry. Cells with epithelial nature were trypsinized and cultured on ECM gel. At the end ultra structure of cells in parallel with tissue were prepared for TEM

Results: Our results showed that the plastic cultured cells have no signs of differentiation and appeared as elongated spindle cell in sections, whereas those cultured on ECM gel had highly differentiated structure and observed as columnar in shape. In this term they were very similar to epithelial cells from tissue fragment. Epithelial cells of oviduct, cultured on ECM gel, were noticed ultra structurally very similar to that from uterus. The main structural difference existed in vivo state [the presence of abundance cilia on apical surface of oviduct epithelial cells] were not observed in vitro

Conclusion: As a conclusion, it seems that ECM gel by itself is enough to induce morphologic differentiation and structural polarization of epithelial cells. Ultra structurally different cells grows and acquires the same structure when being cultured under the same microenvironment

Retrograde ureteroscopic lithotripsy plus extracorporeal shock wave lithotripsy for treatment of relatively large renal pelvis stones

Renal stones are usually treated through ESWL or through skin nephrostomy. This study was conducted to compare between efficacy and safety of retrograde ureteroscopic lithotripsy with pneumatic lithotriptor plus extracorporeal shock wave lithotripsy versus extracorporeal shock wave lithotripsy alone for treatment of 2 to 3 cm renal pelvis stones. This randomized clinical trial was conducted on the population of patients with 2-3 cm renal pelvis stones admitted to the lmam Reza Hospital clinic for radiological patients from Shahrivar 1382 to Shahrivar 1384 [2004-2005]. Sampling procedure included patients with such a diagnosis who had indications for the suggested treatment procedure. The final sample amounted to 55 patients. We divided our patients into two groups. In group 1, [22 patients] first we attempted to break the nidus of the stone with retrograde ureteroscopic pneumatic lithotripsy, and then we inserted a double J stent and after 2 to 4 weeks, send them for extracorporeal shock wave lithotripsy. Group II patients [33 patients], after cystoscopic insertion of double J stent, underwent extracorporeal shock wave lithotripsy alone. Stone free rate and complication rate after three months were recorded. The patients' mean age in two groups were 28.7 and 29.4 years respectively. There was no statistical differences between the age and sex of the two groups. In group 1, in two patients we could not reach to stone ureteroscopically. Stone free rate in group 1, was 77% [17/22]. In group II, after two sessions of extracorporeal lithotripsy, stone free rate was just 45% [15/33]. There was no significant complication in two groups. The present study demonstrated that ureteroscopy and breaking the stone nidus with pneumatic Iithotripter before extracorporeal shock wave lithotripsy is an acceptable treatment modality for relatively large renal pelvis stone

relationship between random urinary protein-to-creatinine ratio and 24-hours urine protein in diagnosis of proteinuria in mild preeclampsia

The purpose of this study was to evaluate whether a random urinary protein / creatinine ratio is a clinically useful predictor of significant proteinuria [>/= 300 mg/24 hr] instead of 24- hours urine protein, among women with suspected preeclampsia. Women with suspected preeclampsia and gestational age of >/= 20 weeks were included in a prospective study. Patients with chronic hypertension, diabetes mellitus, or preexisting renal disease were excluded. Protein/ creatinine ratio was obtained before 24-hours urine collection. Positive and negative predictive values and sensitivity and specificity of the protein/creatinine ratio for significant proteinuria [>/= 300 mg] were calculated, based on 24- hours urine total protein. 100 women were evaluated totally. Mean maternal and gestational ages were 27.3 years and 33.26 weeks, respectively. 73% of cases had significant proteinuria based on 24-hours urine collection. Good correlations were found between the protein/creatinine ratio in random urine samples and both the 24-hours urine protein excretion and the 24- hours urine protein/creatinine ratio in patients with mild preeclampsia [r=0.484, P<0.0001, and r=0.345, P<0.0001, respectively] .Receiver operator characteristic [ROC] analysis revealed an area under the curve of 0.944. The best cutoff value was of >0.18 which yields a sensitivity of 86.3%, a specificity of 100%, with a positive predictive value of 100%, and a negative predictive value of 73%. The random urinary protein .to- creatinine [P: C] ratio is strongly associated with the 24-hours total protein excretion. A cutoff value of > 0.18 is a good predictor of significant proteinuria .P: C ratio could replace the 24- hours urine collection as a simpler, faster, and more accurate method for the diagnosis of significant proteinuria