ABSTRACT
Onosma dichroanthum Boiss. is one of the most important mountainous medicinal plants in Iran. This study was done to determine the biomechanical evaluation of root extract ointment of Onosma dichroanthum Boiss. on wound healing in rats. In this experimental study, 18 male adult Wistar rats were randomly allocated into control [I], vehicle [II] and treated group with ointment containing of extract 1% of root of Onosma dichroanthum Boiss, [III]. 20 mm vertical skin incision wound were made on rats back side. The assessment of the wound healing was carried out at day 14. At the end of study, rats were sacrificed, skin sample were extracted and evaluated by biomechanical method [maximum force, elastic stiffness, energy absorption]. There was no significant difference in biomechanical parameters among the treated, vehicle and control groups. Topical application of Onosma dichroanthum Boiss. root have no effect on healing of skin wound in animal model
Subject(s)
Wound Healing , Plant Extracts/pharmacology , Plant Roots , Plant Preparations , Plant Extracts , Models, Animal , Rats, Wistar , Plants, MedicinalABSTRACT
Low level laser therapy is suggested as a new therapeutic method in diabetic wound healing. This survey aimed to evaluate the effects of low level laser on human fibroblasts cultured in high glucose cultures. The human skin fibroblasts were cultured under standard condition. The cells were cultured in high glucose culture medium [15mM/L] for a week and two weeks before laser irradiation. The irradiation was carried out with Helium-Neon [He-Ne] laser unit on the case groups. Control cells did not receive laser. The cells were irradiated with three doses of 0.5, 1 and 2 J/cm2 on three consecutive days. The releasing rates of Interleukin 6 and Fibroblast growth factor were evaluated by ELISA [enzyme-linked immunoabsorbent assay]. Data were analyzed by student t test or Mann-Whitney-U test. All three doses of 0.5 [p=0.049], 1 J/cm2 [p=0.027] had a stimulatory effect on releasing rate of IL-6 of the fibroblasts cultured in culture medium with high glucose concentration in comparison with hyper glycemic non irradiated cells. Low level laser therapy [LLLT] with energy density of 2 J/cm2 induced the release of bFGF of the fibroblasts cultured in culture medium with high glucose concentration for one and two weeks [p=0.047 and p=0.04 respectively]. This study showed that LLLT has stimulatory effects on the release of Interleukin 6 and basic fibroblast growth factor from cultured human fibroblasts in high glucose medium in one week and two-week tests
ABSTRACT
The aim of the present investigation was to study the effect of pentoxifylline administration on wound healing process of an experimental pressure sore in rat by biomechanical evaluating method. In ten adult male rats under general anesthesia and sterile conditions one experimental pressure sore by no20Halsted mosquito forceps were made. A double layer folded skin of dorsal region were held under most pressure grade of the forceps for 2 hours. It was followed by 30 minutes[the skin] relaxation. This procedure was repeated for 12 times during three consecutive days. Seven days later pressure sore was made. Rats were divided into control and experimental groups. Pentoxifylline was injected intraperitonealy at a dose of 50mg/kg daily from beginning of pressure sore for twenty days. After these procedures rats were killed by chloroform, and samples were extracted from the wound and surrounding skin. Samples were biomechamcally examined by a material testing instrument and maximum stress [N/mm[2]] work up to maximum force [Nmm]. The young modulus of elasticity [N/mm] were them calculated. In experimental group maximum stress [2.05 +/- 0.15] and work up to maximum force [63.75 +/- 4.97] were significantly higher than those of control group[1.3 +/- 0.27] and[43.3 +/- 14.96] [p-0.002 and p=0.035 relatively]. Pentoxifylline administration in rats which had an experimental pressure sore significantly accelerated wound healing process compared to those of control group which was observed by biomechanical examination