ABSTRACT
Background: SP-A and SP-D are hydrophilic proteins which regulate the inflammatory response of the lung. Pasteurella multocida is one of the most common bacteria isolated from calves suffering from shipping fever pneumonia, one of the majorproblems in dairy herds
Objectives: evaluation of surfactant content may provide a valuable diagnostic tool for detection of calf pneumonia due to Pasteurella multocida and also state of treatment
Methods: ten Holstein-Frisian bull calves aged 4 months with body weight of 120 +/- 5 kg were selected for study in two groups. The Pasteurella multocida [PMC66 Razi] was used in the present study for inducing pneumonia. The Bronchoalveolar lavage [BAL] process was done in selected calves. BAL fluid was collected and centrifuged and finally the sediment [crude surfactant] was reserved at -20degreeC. The cytological evaluation and surfactant content was assayed by ELISA, TPL kit assay and HPLC
Results: the serum levels of SP-A and SP-D in pneumonic group were significantly elevated. Although the increased Bronchoalveolar lavage fluid [BALF] level of SP-A in pneumonic cases was found as compared with the control animals, the statistical analysis did not show any significant differences between two groups. The level of SP-D in BALF of pneumonic group significantly elevated. The amount of Dipalmitoylphosphatidylcholine [DPPC] in pneumonic group decreased significantly in comparison with control group
Conclusions: pasteurella inducing pulmonary can change the major component of lung surfactant, evaluation of these markers can be helpful as an appropriate tool in diagnostic state of pneumonia and healing
ABSTRACT
Hydatidosis or cystic hydatid disease is one of the most important diseases in human and animals. Identification of strains is important for improvement of control and prevention of disease. The aim of this study was to determine the strains isolated from human and domestic animals in Ilam Province, Iran, using PCR-RFLP method. Respectively, 30 and 4 animal and human hydatid cysts were collected from different slaughterhouses and hospitals of the province. Protoscolices were separated and their DNA genome was extracted by extraction kit. rDNA-ITS1 of each isolated samples was duplicated by BD1 [Forward] and 4s [Reverse] Primers. PCR products were studied by electrophoresis and then were digested using TaqI, HpaII, Rsal and Alul restriction enzymes. RFLP products were studied using electrophoresis on 1% agar gel. A fragment of l000bp was produced from amplification of rDNA-ITS1 of protoscolices using PCR method. After digestion of PCR product by Alul enzyme, 200bp and 800bp, by Rsal, 655bp and 345bp and by HpaII 700bp and 300bp sizes were obtained. TaqI enzyme had no change in fragment size and it remained l000bp. Considering the method. Ham strains was specified as E. granulosus sensu stricto [G1-G3]. Although sheep strain [G1] is dominated in human and different animal in Iran and the world, but more efforts should be done to clarify the true genotype of Ilam strains specified as E. granulosus sensu stricto [G1-G3]