ABSTRACT
Released dopamine from dopaminergic neurons in the substantia nigra pars compacta affects dentate gyrus [DG] neurogenesis in the hippocampus [HPC]. Damage to dopaminergic neurons in Parkinson's disease [PD] causes decreased neurogenesis in DG which results in memory impairment. This was an experimental laboratory study. We assessed the effect of intravenous transplantation of adipose-derived stem cells [ADSCs] on hippocampal neurogenesis after inducing injury by 6-OHDA [memory disability model of PD].We performed bilateral injections of 6-OHDA into substantia nigra [SNc] of male Wistar rats. First group of the rats received bilateral injections of 6-OHDA [6 micro g] dissolved in 2 micro l saline. Second group received saline injections instead of neurotoxin [sham group]. In the third group we transplanted the 3[rd] passage of ADSC cells which had been assessed for CD90 immunostaining [1×10[6] in 500 microl medium], via tail vein. The 4[th] group included injured rats which received an injection of the fluid of the culture media [500 micro l] through tail vein. After treatment, rats were sacrificed. The brains of the rats were removed, fixed with 4% paraformaldehyde, dehydrated, embedded in paraffin and cut into 10 micro m thick slices. We stained the sections with cresyl violet and determined the density of neurons in DG, CA1, CA3. Statistical analysis was performed by one-way ANOVA and Tukey test. P
ABSTRACT
Epidermis is the outer layer of skin, regenerating continuously. Epidermal stem cells play important roles in tissue regeneration, scar regeneration and neoplasm formation.This study was displayed for the isolation and culture of interfollicular epidermal stem cells from newborn mouse skin without feeder layer. This experimental study was displayed on 0-3 old-day newborn NMRI mouse skin 60-70 gr weight. The epidermal keratinocytes were separated mechanically and enzymatically from 0-3 old day newborn mice skin [NMRI strain] and seeded on fibronectin-collagen culture substrates. Putative epidermal stem cells were selected by rapid adherence for 10 minutes on this composite matrix of type 1 collagen and fibronectin and the unattached cells were discarded and attached cells were cultured in essential minimal eagle medium [EMEM] [ca+2-free culture medium containing 0.05 mM Ca+2, 9% FBS, 50% conditioned medium, EGF [epidermal growth factor] and Cholera Toxin. The immunocytochemistry of beta1-integrin analysis used to indicate their stemness nature. The results indicated that rapid adherence yields 50% purity. By using this method, the stem cells have been subcultured continuously without any change in the cell properties. The isolated interfollicular epidermal stem cells, expressed epidermal stem cells special marker [beta1-integrin] in high levels, which indicates stem cell nature. This new method yields pure viable epidermal stem cells that can be used in regenerative medicine and cell therapy
ABSTRACT
Background: Adipose tissue represents an accessible source of mesenchymal stem cells [Adipose tissue derived mesenchymal stem cells-ADSCs]. ADSCs could be differentiated into various mesenchymal stem cells [e.g. chondrocytes, adipocytes, osteoblasts, and neuronal lineages]. Neurotrophic factors are diffusible polypeptides that have a critical role in survival, proliferation and differentiation of stem cells. This study aimed to evaluate the proliferative capacity and the expression of some neurotrophic factors such as brain-derived neurotrophic factor [BDNF], ciliary neurotrophic factor [CNTF], glial cell-derived neurotrophic factor [GDNF], neurotrophin-3 [NT-3], neurotrophin-4 [NT-4], nerve growth factor [NGF] and the expression of nestin in ADSCs
Materials and Methods: Rat ADSCs were isolated from the subcutaneous adipose tissue using mechanical and enzymatic digestion. These cells were cultured in alpha MEM supplemented with 10% fetal bovine serum. ADSCs of the third passage were evaluated by immunocytochemistry to detect the cell surface markers of CD90 and nestin. Reverse transcription-polymerase chain reaction [RT-PCR] was used to study the expression of the above-mentioned neurotrophic factors in the ADSCs. Moreover, adipogenic and chondrogenic differentiation of ADSCs were induced in-vitro
Results: The assessment of ADSCs identity and purity showed that 90% and 80% of the stem cells were positive for CD90 marker and nestin, respectively. According to RT-PCR results, the above-mentioned neurotrophic factors were expressed in these stem cells. Furthermore, a small number of cells were positive for cresyl violet staining
Conclusion: Adipose tissue contains a stem cell population that seems to be a good multipotential cell candidate for cell replacement therapy