Serological diagnosis of Neospora caninum infection in some domestic animals from Egypt

This study investigated the presence of antibodies to N. caninum in cattle, sheep, goats and dogs from Egypt. Sera from the tested animals were examined using a direct agglutination test incorporating mercaptoethanol and formalin-preserved whole N. caninum tachyzoites as antigen. Anti-N. caninum antibodies were found in 18 out of 111 aborted cattle. Abortion occurred during the third to ninth month of the gestation period. Three out of the 26 non-aborted cattle were seropositive. The statistical analysis indicated that anti-N. caninum antibodies occurred more in the aborted than in the non-aborted group [slightly significant]. Seventy-three of 202 sheep and 31 of 88 goats recorded antibodies for N. caninum. Eight out of 29 stray dogs had antibodies against N. caninum

Studies on Rhinoestrus sp [diptera: Oestridae] larvae infesting donkeys [Equus asinus] in Egypt 1 Incidence and seasonal variations

Examination of 144 donkey heads at the postmortem during the period from September 1989 up to the end of August 1990 revealed that 88 [61.11%] donkeys were infested with rhinoestrus purpureus. The larvae were present in the 88 heads examined throughout the year with highest mean larval number during June [57.86]. The larvae were absent during November and December. The monthly prevalence of each 1st, 2nd and 3rd instar larvae was determined. Each larval instar occurred in two peak of infestations; March and June for the 1st and 2nd 1arval stages and March and August for the 3rd larval stage. The size of infestation showed that the percentage of donkeys infested with 1-50, 51-100 and 101-150 1arvae were 50.69, 9.03 and 1.39% respectively. It was concluded that, two generations of R. purpureus occurred during the year and the adult flies were active during two seasons; from mid-January to mid-April and from early June to late September. Two annual treatment of donkeys during March and June were recommended to eliminate most of the 1arvae from infested donkeys

Epimastigote stage in the larvae of cephalopina titillator [Diptera: Oestridae] infesting camels [Camelus dromedarius] infected with trypanosoma evansi

The present study revealed that all the 3 larval stages of C. titillator collected from T. Evansi infected camels were heavily infected with epimastigote stage. This epimastigote stage was found always in a dividing stage. Experimental infection of mice and guinea pigs with this epimastigote revealed negative results. Further studies are needed to clarify the role of C. titillator in the epidemiology of T. evansi

Detection of encephalitozoonosis and toxoplasmosis among rabbits by carbon immunoassay

Examination of 100 rabbits by carbon immunoassay using mixed antigen of Encephalitozoom cuniculi and toxoplasma gondii revealed that 68% and 20% of rabbits were positive to these parasites respectively. Also, examination of 50 rabbits for E. cuniculi using E. cuniculi antigen suspension showed that 33 [66%] of them were seropositive to this parasite

Cryptosporidiosis among fresh water fish in Egypt

A total number of 41 Clarias lazera and 20 Tilapia nilotica were caught from small branches of the River Nile at Toukh-Qualiobia Governorate Egypt, during the period from 1st September 1989 to 1st march 1990. Mucosal smears from the small and large intestine of each fish were prepared and stained with modified Ziel Neelsen stain. For confirmation of positive cases another smears from each case was stained by Safranin methylene blue stain. Cryptosporidium oocysts were recorded in clarias lazera [21.95%] while tilapia nilotica were free. Experimental inoculation of each of 10 mice, one day old with 5000 oocysts obtained from positive cases revealed negative results

Histopathological studies on donkeys infested with larvae Gasterophilus species

The histopathological alterations of infested parts with different larval stages of Gasterophilus spp. were studied in details. The 1st-instar larvae of G. intestinalis and G. pecorum were located in the tongue. The 2nd-instars larvae of G. pecorum were found only in the pharynx, while those of the 3rd instars were observed in both pharynx and stomach. The 2nd and 3rd instar larvae of G. intestinalis and G. nasalis were found in the stomach and duodenum respectively. It has been concluded that gasterophilus spp. Larvae cause eosinophilic reaction with reactive fibroplasia and necrosis in the tongue, pharynx, stomach and duodenum. Ulcer formation in the mucosa of stomach and duodenum were observed