ABSTRACT
Saliva contamination is the main reason for the failure of sealant therapy. The aim of this study was to evaluate the effect of three types of adhesives [Single Bond, Adhes and I Bond] on sealant microleakage in saliva-contaminated enamel. In this experimental study, 54 intact premolars were chosen and divided into 5 groups. Group 1: teeth were etched and sealed with sealant. In groups 2, 3 and 4: teeth were etched and exposed to saliva for 10 seconds and air-dried. Subsequently, a bonding agent [Single Bond, Adhes and I Bond] was applied prior to the sealant application and curing. In group 5: after being etched and contaminated, the teeth were sealed without any bonding agent. After thermocycling, the teeth were immersed in fuchsin 0.5% as a leakage tracer for 24h.Then the teeth were sectioned in a longitudinal direction. Samples were then evaluated using stereomicroscope. The findings were statistically analyzed using Kruskal - Wallis and Dunn tests. The extent of microleakage in saliva contaminated sealant without bonding agent group had significant difference compared to the Single Bond, Adhes and I Bond groups [p<0.05]. Although the amount of microleakage in study groups was less than the non-contaminated fissure sealant group, the difference was not significant. Besides, there was no significant difference between Single Bond, Adhes and I Bond groups. Exposing enamel to saliva after etching would increase the microleakage. Using Single Bond, Adhes and 1 Bond the dentin adhesives, after salivary contamination would decrease the microleakage to the extent of uncontaminated sealant group