Toxoplasma gondii infection in neonates

To study toxoplasmosis in neonates using PCR and serological methods. Sera and CSF of 104 neonates, hospitalized in infants' ward of Taleghani Hospital, Tehran, Iran were examined. The sera were examined for anti Toxoplasma gondii lgM and lgG specific antibodies with ELISA and IFA techniques, respectively. Meanwhile, obtained CSFs of the cases were evaluated for the genome of this parasite by PCR technique. Results showed positivity in 7 neonates [6.73%] which suggested congenital toxoplasmosis. Results of PCR were positive in 6 neonates [5.77%]. The 1/100 titer of lgM specific antibodies was positive in 5[4.81%] of them by IFA technique and 6 neonates [5.77%] had positive results by ELISA technique for IgM specific. The rate of mortality was 0.96%. Forty one neonates had 1/200 titer of specific lgG antibodies by IFA technique and 38 neonates had positive results by ELISA technique for IgG antibodies. The prevalence of chronic toxoplasmosis in mother was 32.7% and 30% by IFA and ELISA techniques, respectively. Toxoplasmosis is still highly prevalent in neonates and should be considered due to the fact that suspected cases might be misdiagnosed and subsequently led to life- threatening or fatal condition

Seroepidemiology assay of toxoplasmosis in infants who were confined to bed in infants ward of Taleghani hospital during 1379-1380

As some of the mortality and morbidity of infants who are less than one year, is related to contamination with Toxoplasma gondii parasite during fetal time, the aim of this survey is to study the prevalence of Toxoplasmosis in infants visiting Taleghani medical center during 1379-1380. In this survey the sera of 106 and 104 newborn infants from 1 day to 1 month who were suspected to congenital Toxoplasmosis and were hospitalized in Infantile ward of Taleghani hospital were evaluated for IgM and IgG specific antibodies with ELISA and IFA techniques, respectively. According to the results of IFA technique, if the 1/100 is the minimum titer for specific Ig M antibody against Toxoplasmosis, 5 infants were positive and if 1/200 is the minimum titer for specific Ig G antibody against Toxoplasmosis, 41 infants were positive. 6 infants had specific Ig M antibody and 38 infants had specific IgG antibody against Toxoplasmosis by ELISA technique. As a whole, 6 infants [5.66%] were positive which were suggested congenital Toxoplasmosis. With deletion of 6 infected infants, the prevalence of chronic Toxoplasmosis between the mother whose infant was confined in Taleghani hospital was 34 percent by IFA and 30.2 percent by ELISA technique

[Seroepidemiology assay of toxoplasmosis in infants who was confined to bed in infants ward of Taleghani hospital during 1379-1380]

Background: As some of the mortality and morbidity of infants who are less than one y ear, is related to contamination with Toxoplasma gondii parasite during fetal time, the aim of this survey is to study the prevalence Toxoplasmosis in infants visited Taleghani medical center during 1379-1380

Materials and methods: In this survey the sera of 106 and 104 newborn infants from 1 day to 1 month who were suspected to congenital Toxoplasmosis and were hospitalized in Infantile ward of Taleghani hospital were evaluated for lgM and lgG specific antibodies with ELISA and IFA techniques, respectively

Results: According to the results of IFA technique, if the 1/100 is the minimum titer for specific lg M antibody against Toxoplasmosis, 5 infants were positive and if 1/200 is the minimum titer for specific lgG antibody against Toxoplasmosis, 41 infants were positive. 6 infants had specific lg M antibody and 38 infants had specific lgG antibody against Toxoplasmosis by ELISA technique

Conclusions: As a whole, 6 infants [5.66%] were positive which were suggested congenital Toxoplasmosis. With deletion of 6 infected infants, the prevalence of chronic Toxoplasmosis between the mother whose infants was confined in Taleghani hospital was 34 percent by IFA and 30.2 percent by ELISA technique